Cyclophilin 40 Antibody | Affinity Biosciences

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Western blot analysis of extracts from K562, using Cyclophilin 40 Antibody.

Product:	Cyclophilin 40 Antibody
Catalog:	DF13246
Description:	Rabbit polyclonal antibody to Cyclophilin 40
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	40kDa; 41kD(Calculated).
Uniprot:	Q08752
RRID:	AB_2846265

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(88%), Xenopus(88%)

Clonality:

Polyclonal

Specificity:

Cyclophilin 40 Antibody detects endogenous levels of total Cyclophilin 40.

RRID:

AB_2846265
Cite Format: Affinity Biosciences Cat# DF13246, RRID:AB_2846265.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

40 kDa peptidyl prolyl cis trans isomerase; 40 kDa peptidyl prolyl cis trans isomerase D; 40 kDa peptidyl-prolyl cis-trans isomerase; Cyclophilin D; Cyclophilin related protein; Cyclophilin-40; Cyclophilin-related protein; Cyclophilin40; CyclophilinD; CYP 40; CYP-40; CYP40; CYPD; MGC33096; Peptidyl Prolyl Isomerase D; Peptidyl-prolyl cis-trans isomerase D; Peptidylprolyl isomerase D; PPIase; PPIase D; Ppid; PPID_HUMAN; Rotamase; Rotamase D;

Immunogens

Immunogen:

Uniprot:

Q08752(PPID_HUMAN) >>Visit HPA database.

Gene(ID):

PPID(5481)

Expression:

Q08752 PPID_HUMAN:

Widely expressed.

Sequence:

Q08752 PPID_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSHPSPQAKPSNPSNPRVFFDVDIGGERVGRIVLELFADIVPKTAENFRALCTGEKGIGHTTGKPLHFKGCPFHRIIKKFMIQGGDFSNQNGTGGESIYGEKFEDENFHYKHDREGLLSMANAGRNTNGSQFFITTVPTPHLDGKHVVFGQVIKGIGVARILENVEVKGEKPAKLCVIAECGELKEGDDGGIFPKDGSGDSHPDFPEDADIDLKDVDKILLITEDLKNIGNTFFKSQNWEMAIKKYAEVLRYVDSSKAVIETADRAKLQPIALSCVLNIGACKLKMSNWQGAIDSCLEALELDPSNTKALYRRAQGWQGLKEYDQALADLKKAQGIAPEDKAIQAELLKVKQKIKAQKDKEKAVYAKMFA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Rabbit

100

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q08752 As Substrate

Q08752

Site	PTM Type	Source
S5	Phosphorylation	Uniprot
K9	Ubiquitination	Uniprot
K43	Sumoylation	Uniprot
K43	Ubiquitination	Uniprot
K56	Ubiquitination	Uniprot
K64	Ubiquitination	Uniprot
K69	Ubiquitination	Uniprot
K79	Ubiquitination	Uniprot
K102	Sumoylation	Uniprot
K111	Ubiquitination	Uniprot
K154	Ubiquitination	Uniprot
K168	Acetylation	Uniprot
K168	Sumoylation	Uniprot
K168	Ubiquitination	Uniprot
K195	Ubiquitination	Uniprot
S198	Phosphorylation	Uniprot
S201	Phosphorylation	Uniprot
K214	Ubiquitination	Uniprot
K218	Ubiquitination	Uniprot
T223	Phosphorylation	Uniprot
K227	Ubiquitination	Uniprot
K235	Methylation	Uniprot
K235	Ubiquitination	Uniprot
Y246	Phosphorylation	Uniprot
R251	Methylation	Uniprot
Y252	Phosphorylation	Uniprot
K257	Ubiquitination	Uniprot
K267	Ubiquitination	Uniprot
S274	Phosphorylation	Uniprot
C275	S-Nitrosylation	Uniprot
C282	S-Nitrosylation	Uniprot
K283	Ubiquitination	Uniprot
K285	Ubiquitination	Uniprot
S287	Phosphorylation	Uniprot
K308	Ubiquitination	Uniprot
K321	Ubiquitination	Uniprot
Y323	Phosphorylation	Uniprot
K331	Ubiquitination	Uniprot
K341	Sumoylation	Uniprot
K341	Ubiquitination	Uniprot
K349	Ubiquitination	Uniprot
Y365	Phosphorylation	Uniprot
K367	Ubiquitination	Uniprot

Research Backgrounds

Q08752_PPID_HUMAN

Function:

PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding. Proposed to act as a co-chaperone in HSP90 complexes such as in unligated steroid receptors heterocomplexes. Different co-chaperones seem to compete for association with HSP90 thus establishing distinct HSP90-co-chaperone-receptor complexes with the potential to exert tissue-specific receptor activity control. May have a preference for estrogen receptor complexes and is not found in glucocorticoid receptor complexes. May be involved in cytoplasmic dynein-dependent movement of the receptor from the cytoplasm to the nucleus. May regulate MYB by inhibiting its DNA-binding activity. Involved in regulation of AHR signaling by promoting the formation of the AHR:ARNT dimer; the function is independent of HSP90 but requires the chaperone activity. Involved in regulation of UV radiation-induced apoptosis. Promotes cell viability in anaplastic lymphoma kinase-positive anaplastic large-cell lymphoma (ALK+ ALCL) cell lines.

(Microbial infection) May be involved in hepatitis C virus (HCV) replication and release.

Subcellular Location:

Cytoplasm. Nucleus>Nucleolus. Nucleus>Nucleoplasm.

Tissue Specificity:

Widely expressed.

Subunit Structure:

Identified in ESR1 or NR3C1/GCR steroid receptor-chaperone complexes. Found in HSP90 chaperone complexes with kinase clients LCK or EIF2AK1. Two monomers associate with one HSP90 homodimer. Interacts with HSP90AA1. Interacts with HSP90AB1; PPID and FKBP4 compete for binding to HSP90AB1 and the interaction is mutually exclusive with the PPID:HSPA8 interaction. Interacts with HSPA8; PPID and STIP1 but not FKBP4 compete for binding to HSPA8 and the interaction is mutually exclusive with the PPID:HSP90AB1 interaction. Interacts with S100A1 and S100A2; the interactions dissociate the PPID:HSP90AA1 interaction. Interacts with S100A6. Interacts with MYB, ILF2, XRCC6, RACK1 and RPS3. Interacts with cytoplasmic dynein 1 intermediate chain (DYNC1I1 or DYNC1I2).

Family&Domains:

Belongs to the cyclophilin-type PPIase family. PPIase D subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)

Restrictive clause

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Cyclophilin 40 Antibody - #DF13246