Kv4.3 Antibody | Affinity Biosciences

WB
IF/ICC
Cites

1/4

Western blot analysis of extracts from IMR32, using Kv4.

Western blot analysis of extracts from various samples, using Kv4.

DF13239 staining Hela cells by IF/ICC.

Fig.

Product:	Kv4.3 Antibody
Catalog:	DF13239
Description:	Rabbit polyclonal antibody to Kv4.3
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.:	73kDa; 73kD(Calculated).
Uniprot:	Q9UK17
RRID:	AB_2846258

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(88%), Horse(88%), Sheep(88%), Rabbit(100%), Chicken(100%), Xenopus(88%)

Clonality:

Polyclonal

Specificity:

Kv4.3 Antibody detects endogenous levels of total Kv4.3.

RRID:

AB_2846258
Cite Format: Affinity Biosciences Cat# DF13239, RRID:AB_2846258.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

KCND 3; KCND 3L; KCND 3S; Kcnd3; KCND3_HUMAN; KCND3L; KCND3S; KSHIVB; Kv 4.3; MGC14203; MGC142037; OTTHUMP00000063948; Potassium ioinic channel Kv4.3; Potassium voltage gated channel Shal related subfamily member 3; Potassium voltage gated channel subfamily D member 3; Potassium voltage-gated channel subfamily D member 3; Sha1 related potassium channel Kv4.3; Voltage gated K+ channel; Voltage gated potassium channel Kv4.3; Voltage gated potassium channel subunit Kv4.3; Voltage-gated potassium channel subunit Kv4.3;

Immunogens

Immunogen:

Uniprot:

Q9UK17(KCND3_HUMAN) >>Visit HPA database.

Gene(ID):

KCND3(3752)

Expression:

Q9UK17 KCND3_HUMAN:

Highly expressed in heart and brain, in particular in cortex, cerebellum, amygdala and caudate nucleus. Detected at lower levels in liver, skeletal muscle, kidney and pancreas. Isoform 1 predominates in most tissues. Isoform 1 and isoform 2 are detected at similar levels in brain, skeletal muscle and pancreas.

Sequence:

Q9UK17 KCND3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAGVAAWLPFARAAAIGWMPVANCPMPLAPADKNKRQDELIVLNVSGRRFQTWRTTLERYPDTLLGSTEKEFFFNEDTKEYFFDRDPEVFRCVLNFYRTGKLHYPRYECISAYDDELAFYGILPEIIGDCCYEEYKDRKRENAERLMDDNDSENNQESMPSLSFRQTMWRAFENPHTSTLALVFYYVTGFFIAVSVITNVVETVPCGTVPGSKELPCGERYSVAFFCLDTACVMIFTVEYLLRLFAAPSRYRFIRSVMSIIDVVAIMPYYIGLVMTNNEDVSGAFVTLRVFRVFRIFKFSRHSQGLRILGYTLKSCASELGFLLFSLTMAIIIFATVMFYAEKGSSASKFTSIPASFWYTIVTMTTLGYGDMVPKTIAGKIFGSICSLSGVLVIALPVPVIVSNFSRIYHQNQRADKRRAQKKARLARIRVAKTGSSNAYLHSKRNGLLNEALELTGTPEEEHMGKTTSLIESQHHHLLHCLEKTTGLSYLVDDPLLSVRTSTIKNHEFIDEQMFEQNCMESSMQNYPSTRSPSLSSHPGLTTTCCSRRSKKTTHLPNSNLPATRLRSMQELSTIHIQGSEQPSLTTSRSSLNLKADDGLRPNCKTSQITTAIISIPTPPALTPEGESRPPPASPGPNTNIPSIASNVVKVSAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Chicken

100

Horse

Bovine

Sheep

Xenopus

Pig

Dog

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UK17 As Substrate

Q9UK17

Site	PTM Type	Enzyme	Source
Y108	Phosphorylation	P12931 (SRC)	Uniprot
Y136	Phosphorylation	P00533 (EGFR)	Uniprot
S153	Phosphorylation		Uniprot
T288	Phosphorylation		Uniprot
T435	Phosphorylation		Uniprot
Y441	Phosphorylation		Uniprot
T486	Phosphorylation		Uniprot
S499	Phosphorylation		Uniprot
T504	Phosphorylation		Uniprot
T555	Phosphorylation		Uniprot
T565	Phosphorylation		Uniprot
S591	Phosphorylation		Uniprot

Research Backgrounds

Q9UK17_KCND3_HUMAN

Function:

Pore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits.

PTMs:

Regulated through phosphorylation at Ser-569 by CaMK2D.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Cell membrane>Sarcolemma>Multi-pass membrane protein. Cell projection>Dendrite.
Note: Interaction with palmitoylated KCNIP2 and KCNIP3 enhances cell surface expression.

Tissue Specificity:

Subunit Structure:

Homotetramer or heterotetramer with KCND1 and/or KCND2. Associates with the regulatory subunits KCNIP1, KCNIP2, KCNIP3 and KCNIP4 (By similarity). Interacts with KCNE1, KCNE2, SCN1B and KCNAB1 and DLG1.

Family&Domains:

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Belongs to the potassium channel family. D (Shal) (TC 1.A.1.2) subfamily. Kv4.3/KCND3 sub-subfamily.

References

1). Role of Ion Channels in Chronic Intermittent Hypoxia-Induced Atrial Remodeling in Rats. LIFE SCIENCES, 2020 (PubMed: 32417371) [IF=6.1]

Application: WB Species: rat Sample: atrial

Fig. 6.| Protein levels of Nav1.5, Cav1.2, Kv4.2, Kv4.3, Kv1.5, NCX1, RyR2, p-RyR2, CaMKII, p-CaMKII confirmed by Western Blotting in two groups (n = 10). And p-RyR2/RyR2, p-CaMKII/CaMKII were also calculated and compared between two groups. LA -left atrial, RA -right atrial.

2). Rare KCND3 Loss-of-Function Mutation Associated With the SCA19/22. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022 (PubMed: 34067185) [IF=5.6]

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Kv4.3 Antibody - #DF13239