Product: P2X2 Antibody
Catalog: DF13236
Description: Rabbit polyclonal antibody to P2X2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Mol.Wt.: 51kDa; 52kD(Calculated).
Uniprot: Q9UBL9
RRID: AB_2846255

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
P2X2 Antibody detects endogenous levels of total P2X2.
RRID:
AB_2846255
Cite Format: Affinity Biosciences Cat# DF13236, RRID:AB_2846255.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATP receptor; MGC129601; P2RX 2; P2RX2; P2RX2_HUMAN; P2X purinoceptor 2; P2X Receptor subunit 2; P2X2; P2X2a; Purinergic receptor; Purinergic receptor P2X ligand gated ion channel 2; Purinergic receptor P2X2 isoform A; Purinergic receptor P2X2 isoform B; Purinergic receptor P2X2 isoform C; Purinergic receptor P2X2 isoform D; Purinergic receptor P2X2 isoform H; Purinergic receptor P2X2 isoform I;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MAAAQPKYPAGATARRLARGCWSALWDYETPKVIVVRNRRLGVLYRAVQLLILLYFVWYVFIVQKSYQESETGPESSIITKVKGITTSEHKVWDVEEYVKPPEGGSVFSIITRVEATHSQTQGTCPESIRVHNATCLSDADCVAGELDMLGNGLRTGRCVPYYQGPSKTCEVFGWCPVEDGASVSQFLGTMAPNFTILIKNSIHYPKFHFSKGNIADRTDGYLKRCTFHEASDLYCPIFKLGFIVEKAGESFTELAHKGGVIGVIINWDCDLDLPASECNPKYSFRRLDPKHVPASSGYNFRFAKYYKINGTTTRTLIKAYGIRIDVIVHGQAGKFSLIPTIINLATALTSVGVGSFLCDWILLTFMNKNKVYSHKKFDKVCTPSHPSGSWPVTLARVLGQAPPEPGHRSEDQHPSPPSGQEGQQGAECGPAFPPLRPCPISAPSEQMVDTPASEPAQASTPTDPKGLAQL

PTMs - Q9UBL9 As Substrate

Site PTM Type Enzyme
K83 Ubiquitination
Y222 Phosphorylation
Y307 Phosphorylation
T312 Phosphorylation

Research Backgrounds

Function:

Ion channel gated by extracellular ATP involved in a variety of cellular responses, such as excitatory postsynaptic responses in sensory neurons, neuromuscular junctions (NMJ) formation, hearing, perception of taste and peristalsis. In the inner ear, regulates sound transduction and auditory neurotransmission, outer hair cell electromotility, inner ear gap junctions, and K(+) recycling. Mediates synaptic transmission between neurons and from neurons to smooth muscle.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.
Note: Localizes to the apical membranes of hair cells in the organ of Corti.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homotrimer and heterotrimer; functional P2XRs are organized as homomeric and heteromeric trimers.

Family&Domains:

Belongs to the P2X receptor family.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Sensory system > Taste transduction.

References

1). Identification of Calcium Channel-Related Gene P2RX2 for Prognosis and Immune Infiltration in Prostate Cancer. Disease Markers, 2022 (PubMed: 36246559)

Application: WB    Species: Human    Sample: C4-2b and CWR22Rv1 cells

Figure 9 P2RX2 acts as a tumor suppressor in PCa: (a) The expression of P2RX2 in C4-2b and CWR22Rv1 cells after transfection. (b) Cell counting kit-8 for C4-2b and CWR22Rv1 cells after 24 h. (c–d) Scratch assay in the C4-2b and CWR22Rv1 cells. (f–g) Colony formation in C4-2b and CWR22Rv1 cells.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.