Stra8 Antibody | Affinity Biosciences

WB
IHC
IF/ICC
Cites

1/6

Western blot analysis on Mouse testis tissue using STRA8 antibody.

DF13234 at 1/100 staining Rat testis tissue by IHC-P.

DF13234 staining A549 cells by IF/ICC.

FIGURE 13 Validation of regulated signalling pathways, spermatogenic cell apoptosis and proliferation, and meiotic spermatocytes by Western blot.

FIGURE 13| Validation of regulated signalling pathways, spermatogenic cell apoptosis and proliferation, and meiotic spermatocytes by Western blot.

Figure 4 The effect of a high-fat diet on miR-34c-5p and spermatogenesis-related proteins in testicular tissues among DIO-R, DIO, and control mice.

Product:	Stra8 Antibody
Catalog:	DF13234
Description:	Rabbit polyclonal antibody to Stra8
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.:	36 kd; 37kD(Calculated).
Uniprot:	Q7Z7C7
RRID:	AB_2846253

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

Stra8 Antibody detects endogenous levels of total Stra8.

RRID:

AB_2846253
Cite Format: Affinity Biosciences Cat# DF13234, RRID:AB_2846253.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Stimulated by retinoic acid 8; stimulated by retinoic acid 8 homolog; Stimulated by retinoic acid Gene 8; stimulated by retinoic acid gene 8 homolog; Stimulated by retinoic acid gene 8 protein homolog; STRA 8; STRA8; STRA8_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q7Z7C7(STRA8_HUMAN) >>Visit HPA database.

Gene(ID):

STRA8(346673)

Expression:

Q7Z7C7 STRA8_HUMAN:

Expressed specifically in testis and fetal ovaries.

Sequence:

Q7Z7C7 STRA8_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGKIDVDKILFFNQEIRLWQLIMATPEENSNPHDRATPQLPAQLQELEHRVARRRLSQARHRATLAALFNNLRKTVYSQSDLIASKWQVLNKAKSHIPELEQTLDNLLKLKASFNLEDGHASSLEEVKKEYASMYSGNDSFPQNGSSPWYLNFYKQTMDLLTGSGIITPQEAALPIVSAAISHLWQNLSEERKASLRQAWAQKHRGPATLAEACREPACAEGSVKDSGVDSQGASCSLVSTPEEILFEDAFDVASFLDKSEVPSTSSSSSVLASCNPENPEEKFQLYMQIINFFKGLSCANTQVKQEASFPVDEEMIMLQCTETFDDEDL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7Z7C7 As Substrate

Q7Z7C7

Site	PTM Type	Source
T75	Phosphorylation	Uniprot
Y77	Phosphorylation	Uniprot
S85	Phosphorylation	Uniprot

Research Backgrounds

Q7Z7C7_STRA8_HUMAN

Function:

Meiosis-inducer required for the transition into meiosis for both female and male germ cells. In female germ cells, required for premeiotic DNA replication and subsequent events in meiotic prophase. During spermatogenesis, next to its role in meiotic initiation, promotes (but is not required for) spermatogonial differentiation. Can associate with DNA (possibly in an indirect manner), and in vitro can activate DNA transcription (By similarity).

PTMs:

Phosphorylated.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Shuttles between nucleus and cytoplasm. Nuclear export is XPO1-dependent.

Tissue Specificity:

Expressed specifically in testis and fetal ovaries.

Subunit Structure:

Interacts with XPO1.

References

1). Long noncoding RNAs regulated spermatogenesis in varicocele‐induced spermatogenic dysfunction. CELL PROLIFERATION, 2022 (PubMed: 35297519) [IF=8.5]

Application: WB Species: Rat Sample: spermatogenic cell

FIGURE 13 Validation of regulated signalling pathways, spermatogenic cell apoptosis and proliferation, and meiotic spermatocytes by Western blot. Representative Western blot images of PI3K, Akt, p‐Akt, caspase‐9, Bcl‐2, Bax, PCNA, PLZF, REC8, STRA8, and SYCP3. (B) Statistical analysis of band intensity by Student's t‐test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. NS, not significant; Sham, sham group; Surgical treatment, surgical treatment group; VC, varicocele group

Application: WB Species: rat Sample: Testicular

FIGURE 13| Validation of regulated signalling pathways, spermatogenic cell apoptosis and proliferation, and meiotic spermatocytes by Western blot. Representative Western blot images of PI3K, Akt, p-Akt, caspase-9, Bcl-2, Bax, PCNA, PLZF, REC8, STRA8, and SYCP3.

2). Differential Impairment Mechanism of Sperm Production via Induction of miR-34c-Activated Apoptosis and Spermatogenesis Pathway in Diet-Induced Obesity and Resistant Mice and GC-1 Spg Cells. International journal of molecular sciences, 2024 (PubMed: 39000558) [IF=5.6]

Application: WB Species: Mouse Sample: testicular tissues

Figure 4 The effect of a high-fat diet on miR-34c-5p and spermatogenesis-related proteins in testicular tissues among DIO-R, DIO, and control mice. (a) miR-34c-5p levels in testicular tissue (n = 6); (b) representative Western blots using antibodies against RARg, Stra8, and PLZF in testicular tissue (n = 6). (c–e) The bar graphs show the results of the semi-quantitative measurement of RARg, Stra8, and PLZF expression, respectively. All data are expressed as mean ± SD; * p < 0.05, ** p < 0.01, versus control group; # p < 0.05, ## p < 0.01, versus DIO group.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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Stra8 Antibody - #DF13234