Clathrin heavy chain Antibody - #DF13223

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Product: Clathrin heavy chain Antibody
Catalog: DF13223
Description: Rabbit polyclonal antibody to Clathrin heavy chain
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Rabbit, Dog, Chicken
Mol.Wt.: 187kDa; 187kD(Calculated).
Uniprot: P53675
RRID: AB_2846242

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(88%), Rabbit(88%), Dog(100%), Chicken(88%)
Clonality:
Polyclonal
Specificity:
Clathrin heavy chain Antibody detects endogenous levels of total Clathrin heavy chain.
RRID:
AB_2846242
Cite Format: Affinity Biosciences Cat# DF13223, RRID:AB_2846242.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:
Uniprot:

P53675(CLH2_HUMAN) >>Visit HPA database.

Gene(ID):
CLTCL1(8218)
Expression:
P53675 CLH2_HUMAN:

Maximal levels in skeletal muscle. High levels in heart and testis. Low expression detected in all other tissues.

Sequence:
MAQILPVRFQEHFQLQNLGINPANIGFSTLTMESDKFICIREKVGEQAQVTIIDMSDPMAPIRRPISAESAIMNPASKVIALKAGKTLQIFNIEMKSKMKAHTMAEEVIFWKWVSVNTVALVTETAVYHWSMEGDSQPMKMFDRHTSLVGCQVIHYRTDEYQKWLLLVGISAQQNRVVGAMQLYSVDRKVSQPIEGHAAAFAEFKMEGNAKPATLFCFAVRNPTGGKLHIIEVGQPAAGNQPFVKKAVDVFFPPEAQNDFPVAMQIGAKHGVIYLITKYGYLHLYDLESGVCICMNRISADTIFVTAPHKPTSGIIGVNKKGQVLSVCVEEDNIVNYATNVLQNPDLGLRLAVRSNLAGAEKLFVRKFNTLFAQGSYAEAAKVAASAPKGILRTRETVQKFQSIPAQSGQASPLLQYFGILLDQGQLNKLESLELCHLVLQQGRKQLLEKWLKEDKLECSEELGDLVKTTDPMLALSVYLRANVPSKVIQCFAETGQFQKIVLYAKKVGYTPDWIFLLRGVMKISPEQGLQFSRMLVQDEEPLANISQIVDIFMENSLIQQCTSFLLDALKNNRPAEGLLQTWLLEMNLVHAPQVADAILGNKMFTHYDRAHIAQLCEKAGLLQQALEHYTDLYDIKRAVVHTHLLNPEWLVNFFGSLSVEDSVECLHAMLSANIRQNLQLCVQVASKYHEQLGTQALVELFESFKSYKGLFYFLGSIVNFSQDPDVHLKYIQAACKTGQIKEVERICRESSCYNPERVKNFLKEAKLTDQLPLIIVCDRFGFVHDLVLYLYRNNLQRYIEIYVQKVNPSRTPAVIGGLLDVDCSEEVIKHLIMAVRGQFSTDELVAEVEKRNRLKLLLPWLESQIQEGCEEPATHNALAKIYIDSNNSPECFLRENAYYDSSVVGRYCEKRDPHLACVAYERGQCDLELIKVCNENSLFKSEARYLVCRKDPELWAHVLEETNPSRRQLIDQVVQTALSETRDPEEISVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYISRLDNYDALDIASIAVSSALYEEAFTVFHKFDMNASAIQVLIEHIGNLDRAYEFAERCNEPAVWSQLAQAQLQKDLVKEAINSYIRGDDPSSYLEVVQSASRSNNWEDLVKFLQMARKKGRESYIETELIFALAKTSRVSELEDFINGPNNAHIQQVGDRCYEEGMYEAAKLLYSNVSNFARLASTLVHLGEYQAAVDNSRKASSTRTWKEVCFACMDGQEFRFAQLCGLHIVIHADELEELMCYYQDRGYFEELILLLEAALGLERAHMGMFTELAILYSKFKPQKMLEHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEYDNAVLTMMSHPTEAWKEGQFKDIITKVANVELCYRALQFYLDYKPLLINDLLLVLSPRLDHTWTVSFFSKAGQLPLVKPYLRSVQSHNNKSVNEALNHLLTEEEDYQGLRASIDAYDNFDNISLAQQLEKHQLMEFRCIAAYLYKGNNWWAQSVELCKKDHLYKDAMQHAAESRDAELAQKLLQWFLEEGKRECFAACLFTCYDLLRPDMVLELAWRHNLVDLAMPYFIQVMREYLSKVDKLDALESLRKQEEHVTEPAPLVFDFDGHE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Zebrafish
88
Chicken
88
Rabbit
88
Pig
0
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P53675 As Substrate

Site PTM Type Enzyme
K78 Ubiquitination
K83 Ubiquitination
K86 Ubiquitination
K96 Ubiquitination
T103 Phosphorylation
S171 Phosphorylation
T214 Phosphorylation
T224 Phosphorylation
K227 Ubiquitination
K245 Ubiquitination
K246 Ubiquitination
K362 Ubiquitination
K367 Ubiquitination
S386 Phosphorylation
K389 Ubiquitination
K450 Acetylation
K450 Ubiquitination
K453 Ubiquitination
K456 Acetylation
K456 Ubiquitination
S477 Phosphorylation
K506 Acetylation
K507 Ubiquitination
Y510 Phosphorylation
K619 Ubiquitination
S672 Phosphorylation
K737 Ubiquitination
K742 Acetylation
K742 Ubiquitination
Y754 Phosphorylation
K764 Acetylation
K764 Ubiquitination
Y799 Phosphorylation
Y803 Phosphorylation
K806 Ubiquitination
S841 Phosphorylation
T842 Phosphorylation
K851 Ubiquitination
K856 Ubiquitination
S889 Phosphorylation
K911 Ubiquitination
K932 Ubiquitination
K941 Ubiquitination
K951 Ubiquitination
T963 Phosphorylation
S966 Phosphorylation
S989 Phosphorylation
S1016 Phosphorylation
S1019 Phosphorylation
K1034 Ubiquitination
T1038 Phosphorylation
Y1043 Phosphorylation
S1045 Phosphorylation
Y1096 Phosphorylation
K1122 Ubiquitination
Y1168 Phosphorylation
T1171 Phosphorylation
S1181 Phosphorylation
T1318 Phosphorylation
S1341 Phosphorylation
K1347 Ubiquitination
K1387 Ubiquitination
K1392 Ubiquitination
T1396 Phosphorylation
K1449 Acetylation
K1449 Ubiquitination
K1461 Ubiquitination
S1462 Phosphorylation
K1529 Ubiquitination
K1535 Ubiquitination
K1612 Ubiquitination
K1621 Ubiquitination

Research Backgrounds

Function:

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network (By similarity).

Subcellular Location:

Cytoplasmic vesicle membrane>Peripheral membrane protein>Cytoplasmic side. Membrane>Coated pit>Peripheral membrane protein>Cytoplasmic side.
Note: Cytoplasmic face of coated pits and vesicles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Maximal levels in skeletal muscle. High levels in heart and testis. Low expression detected in all other tissues.

Subunit Structure:

Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat. In the presence of light chains, hub assembly is influenced by both the pH and the concentration of calcium (By similarity). May interact with OCRL (By similarity).

Family&Domains:

The C-terminal third of the heavy chains forms the hub of the triskelion. This region contains the trimerization domain and the light-chain binding domain involved in the assembly of the clathrin lattice.

The N-terminal seven-bladed beta-propeller is formed by WD40-like repeats, and projects inward from the polyhedral outer clathrin coat. It constitutes a major protein-protein interaction node (By similarity).

Belongs to the clathrin heavy chain family.

Research Fields

· Cellular Processes > Transport and catabolism > Lysosome.   (View pathway)

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Organismal Systems > Nervous system > Synaptic vesicle cycle.

· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.

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