Citrate synthetase Antibody - #DF13222

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Product: Citrate synthetase Antibody
Catalog: DF13222
Description: Rabbit polyclonal antibody to Citrate synthetase
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 51kDa; 52kD(Calculated).
Uniprot: O75390
RRID: AB_2846241

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Citrate synthetase Antibody detects endogenous levels of total Citrate synthetase.
RRID:
AB_2846241
Cite Format: Affinity Biosciences Cat# DF13222, RRID:AB_2846241.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CISY_HUMAN; Citrate synthase; Citrate synthase, mitochondrial; citrate synthetase; Cs; EC 2.3.3; EC 2.3.3.1;

Immunogens

Immunogen:
Uniprot:

O75390(CISY_HUMAN) >>Visit HPA database.

Gene(ID):
CS(1431)
Sequence:
MALLTAAARLLGTKNASCLVLAARHASASSTNLKDILADLIPKEQARIKTFRQQHGKTVVGQITVDMMYGGMRGMKGLVYETSVLDPDEGIRFRGFSIPECQKLLPKAKGGEEPLPEGLFWLLVTGHIPTEEQVSWLSKEWAKRAALPSHVVTMLDNFPTNLHPMSQLSAAVTALNSESNFARAYAQGISRTKYWELIYEDSMDLIAKLPCVAAKIYRNLYREGSGIGAIDSNLDWSHNFTNMLGYTDHQFTELTRLYLTIHSDHEGGNVSAHTSHLVGSALSDPYLSFAAAMNGLAGPLHGLANQEVLVWLTQLQKEVGKDVSDEKLRDYIWNTLNSGRVVPGYGHAVLRKTDPRYTCQREFALKHLPNDPMFKLVAQLYKIVPNVLLEQGKAKNPWPNVDAHSGVLLQYYGMTEMNYYTVLFGVSRALGVLAQLIWSRALGFPLERPKSMSTEGLMKFVDSKSG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75390 As Substrate

Site PTM Type Enzyme
T5 Phosphorylation
T13 Phosphorylation
K43 Acetylation
K43 Ubiquitination
K76 Acetylation
K76 Ubiquitination
Y80 Phosphorylation
T82 Phosphorylation
S83 Phosphorylation
S97 Phosphorylation
C101 S-Nitrosylation
K103 Acetylation
K103 Ubiquitination
S190 Phosphorylation
K193 Acetylation
C211 S-Nitrosylation
K215 Acetylation
K215 Ubiquitination
K321 Acetylation
K327 Acetylation
K327 Ubiquitination
Y331 Phosphorylation
S338 Phosphorylation
K366 Acetylation
K366 Ubiquitination
K375 Acetylation
Y381 Phosphorylation
K382 Acetylation
K382 Ubiquitination
K393 Acetylation
K393 Ubiquitination
K395 Methylation
K450 Acetylation
S451 Phosphorylation
K459 Acetylation
K459 Ubiquitination
S463 Phosphorylation

Research Backgrounds

PTMs:

Methylated. Trimethylation at Lys-395 by CSKMT decreases citrate synthase activity.

Subcellular Location:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the citrate synthase family.

Research Fields

· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).

· Metabolism > Carbohydrate metabolism > Glyoxylate and dicarboxylate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > 2-Oxocarboxylic acid metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

References

1). A novel HSF1 activator ameliorates non‐alcoholic steatohepatitis by stimulating mitochondrial adaptive oxidation. BRITISH JOURNAL OF PHARMACOLOGY, 2022 (PubMed: 34783017) [IF=7.3]

Application: WB    Species: Human    Sample: LX-2 cells

FIGURE 4 SYSU-3d promotes lipid oxidation by activating the heat shock factor 1 (HSF1)/ PPARγ coactivator-1α (PGC-1ɑ) metabolic pathway (a–e) HepG-2 cells were exposed to palmitic acid (PA; 0.75 mM) in the presence or absence of SYSU-3d (1 μM) for 24 h and then analysed. (a) mtDNA/nDNA. (b) Determination of the extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) from metabolic flux analyses. (c) Free fatty acid (FFA) uptake assay using the probe TF2-C12. (d) ROS level determination by flow cytometry. (e) Expression of citrate synthase (CS), malate dehydrogenase 2 (MDH2) and mitochondrial complexes; protein levels were quantified and normalized to Actin. (f–h) Determination of CS, mitochondrial complex I and II activity by normalizing to protein level. (i–k) Determination of oxygen consumption rate (OCR) ability, mitochondrial copy numbers (indicated by Mito-tracker probe) and ROS level (indicated by DCFH-DA probe) in HSF1−/− cells. (l and m) Determination of triglyceride (TG) levels, cell survival, mitochondrial copy numbers and ROS levels in PGC-1ɑ−/− cells. (n) Determination of OCR in PGC-1ɑ−/− cells. (o) Expression of metabolic regulators and inflammation- and apoptosis-related proteins in PGC-1ɑ−/− cells; protein levels were quantified and normalized to Actin; n = 5 independent experiments. *P

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