Carbonic Anhydrase IX Antibody - #DF13218

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Product: Carbonic Anhydrase IX Antibody
Catalog: DF13218
Description: Rabbit polyclonal antibody to Carbonic Anhydrase IX
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 49kDa; 50kD(Calculated).
Uniprot: Q16790
RRID: AB_2846237

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Carbonic Anhydrase IX Antibody detects endogenous levels of total Carbonic Anhydrase IX.
RRID:
AB_2846237
Cite Format: Affinity Biosciences Cat# DF13218, RRID:AB_2846237.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CA-IX; CA9; CAH9_HUMAN; CAIX; Carbonate dehydratase IX; Carbonic anhydrase 9; Carbonic anhydrase IX; Carbonic dehydratase; G250; Membrane antigen MN; MN; P54/58N; pMW1; RCC associated protein G250; RCC-associated antigen G250; Renal cell carcinoma-associated antigen G250;

Immunogens

Immunogen:
Uniprot:

Q16790(CAH9_HUMAN) >>Visit HPA database.

Gene(ID):
CA9(768)
Expression:
Q16790 CAH9_HUMAN:

Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.

Sequence:
MAPLCPSPWLPLLIPAPAPGLTVQLLLSLLLLVPVHPQRLPRMQEDSPLGGGSSGEDDPLGEEDLPSEEDSPREEDPPGEEDLPGEEDLPGEEDLPEVKPKSEEEGSLKLEDLPTVEAPGDPQEPQNNAHRDKEGDDQSHWRYGGDPPWPRVSPACAGRFQSPVDIRPQLAAFCPALRPLELLGFQLPPLPELRLRNNGHSVQLTLPPGLEMALGPGREYRALQLHLHWGAAGRPGSEHTVEGHRFPAEIHVVHLSTAFARVDEALGRPGGLAVLAAFLEEGPEENSAYEQLLSRLEEIAEEGSETQVPGLDISALLPSDFSRYFQYEGSLTTPPCAQGVIWTVFNQTVMLSAKQLHTLSDTLWGPGDSRLQLNFRATQPLNGRVIEASFPAGVDSSPRAAEPVQLNSCLAAGDILALVFGLLFAVTSVAFLVQMRRQHRRGTKGGVSYRPAEVAETGA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
88
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16790 As Substrate

Site PTM Type Enzyme
T115 O-Glycosylation
Y289 Phosphorylation
T333 Phosphorylation
N346 N-Glycosylation
T443 Phosphorylation P17612 (PRKACA)
S448 Phosphorylation
Y449 Phosphorylation

Research Backgrounds

Function:

Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.

PTMs:

Asn-346 bears high-mannose type glycan structures.

Subcellular Location:

Nucleus. Nucleus>Nucleolus. Cell membrane>Single-pass type I membrane protein. Cell projection>Microvillus membrane>Single-pass type I membrane protein.
Note: Found on the surface microvilli and in the nucleus, particularly in nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.

Subunit Structure:

Forms oligomers linked by disulfide bonds.

Family&Domains:

Belongs to the alpha-carbonic anhydrase family.

Research Fields

· Metabolism > Energy metabolism > Nitrogen metabolism.

References

1). Platelets are recruited to hepatocellular carcinoma tissues in a CX3CL1‐CX3CR1 dependent manner and induce tumour cell apoptosis. Molecular Oncology (PubMed: 32799418) [IF=6.6]

Application: IHC    Species: human    Sample: liver

Fig. 4. |Hypoxia promotes platelet recruitment by upregulating CX3CL1 expression.(C) Expression of CX3CL1 and CA9 in human HCC and NT liver tissues. Representative images of two consecutive slices of the same sample embedded in paraffin were taken, and each was stained with CX3CL1 and CA9 (n = 3). Bar, 200 lm.

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