Clathrin Antibody - #DF13215
Product: | Clathrin Antibody |
Catalog: | DF13215 |
Description: | Rabbit polyclonal antibody to Clathrin |
Application: | WB IHC IF/ICC |
Reactivity: | Human, Mouse, Rat |
Prediction: | Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
Mol.Wt.: | 191kDa; 192kD(Calculated). |
Uniprot: | Q00610 |
RRID: | AB_2846234 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13215, RRID:AB_2846234.
Fold/Unfold
Adapter related protein complex 1 beta 1 subunit; Adaptor Protein Complex AP1 Beta 1 Subunit; ADTB1; AP 1 complex subunit beta 1; AP105A; AP1B1; BAM22; Beta Adaptin 1; Beta Prime Adaptin; CLAPB2; Clathrin Assembly Protein Complex 1 Beta Large Chain; Clathrin heavy chain 1; CLH 17; CLH17; CLTC; CLTCL2; Golgi Adaptor HA1/AP1 Adaptin Beta Subunit; KIAA0034; Plasma Membrane Adaptor HA2/AP2 Adaptor Beta Subunit;
Immunogens
- Q00610 CLH1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICIREKVGEQAQVVIIDMNDPSNPIRRPISADSAIMNPASKVIALKAGKTLQIFNIEMKSKMKAHTMTDDVTFWKWISLNTVALVTDNAVYHWSMEGESQPVKMFDRHSSLAGCQIINYRTDAKQKWLLLTGISAQQNRVVGAMQLYSVDRKVSQPIEGHAASFAQFKMEGNAEESTLFCFAVRGQAGGKLHIIEVGTPPTGNQPFPKKAVDVFFPPEAQNDFPVAMQISEKHDVVFLITKYGYIHLYDLETGTCIYMNRISGETIFVTAPHEATAGIIGVNRKGQVLSVCVEEENIIPYITNVLQNPDLALRMAVRNNLAGAEELFARKFNALFAQGNYSEAAKVAANAPKGILRTPDTIRRFQSVPAQPGQTSPLLQYFGILLDQGQLNKYESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKSVDPTLALSVYLRANVPNKVIQCFAETGQVQKIVLYAKKVGYTPDWIFLLRNVMRISPDQGQQFAQMLVQDEEPLADITQIVDVFMEYNLIQQCTAFLLDALKNNRPSEGPLQTRLLEMNLMHAPQVADAILGNQMFTHYDRAHIAQLCEKAGLLQRALEHFTDLYDIKRAVVHTHLLNPEWLVNYFGSLSVEDSLECLRAMLSANIRQNLQICVQVASKYHEQLSTQSLIELFESFKSFEGLFYFLGSIVNFSQDPDVHFKYIQAACKTGQIKEVERICRESNCYDPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLILVVRGQFSTDELVAEVEKRNRLKLLLPWLEARIHEGCEEPATHNALAKIYIDSNNNPERFLRENPYYDSRVVGKYCEKRDPHLACVAYERGQCDLELINVCNENSLFKSLSRYLVRRKDPELWGSVLLESNPYRRPLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISNELFEEAFAIFRKFDVNTSAVQVLIEHIGNLDRAYEFAERCNEPAVWSQLAKAQLQKGMVKEAIDSYIKADDPSSYMEVVQAANTSGNWEELVKYLQMARKKARESYVETELIFALAKTNRLAELEEFINGPNNAHIQQVGDRCYDEKMYDAAKLLYNNVSNFGRLASTLVHLGEYQAAVDGARKANSTRTWKEVCFACVDGKEFRLAQMCGLHIVVHADELEELINYYQDRGYFEELITMLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEYDNAIITMMNHPTDAWKEGQFKDIITKVANVELYYRAIQFYLEFKPLLLNDLLMVLSPRLDHTRAVNYFSKVKQLPLVKPYLRSVQNHNNKSVNESLNNLFITEEDYQALRTSIDAYDNFDNISLAQRLEKHELIEFRRIAAYLFKGNNRWKQSVELCKKDSLYKDAMQYASESKDTELAEELLQWFLQEEKRECFGACLFTCYDLLRPDVVLETAWRHNIMDFAMPYFIQVMKEYLTKVDKLDASESLRKEEEQATETQPIVYGQPQLMLTAGPSVAVPPQAPFGYGYTAPPYGQPQPGFGYSM
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q00610 As Substrate
Site | PTM Type | Enzyme | Source |
---|---|---|---|
A2 | Acetylation | Uniprot | |
T31 | Phosphorylation | Uniprot | |
S67 | Phosphorylation | Uniprot | |
S70 | Phosphorylation | Uniprot | |
K78 | Ubiquitination | Uniprot | |
K83 | Ubiquitination | Uniprot | |
K86 | Ubiquitination | Uniprot | |
K96 | Ubiquitination | Uniprot | |
T105 | Phosphorylation | Uniprot | |
R144 | Methylation | Uniprot | |
S146 | Phosphorylation | Uniprot | |
C151 | S-Nitrosylation | Uniprot | |
T158 | Phosphorylation | Uniprot | |
K163 | Ubiquitination | Uniprot | |
S171 | Phosphorylation | Uniprot | |
K189 | Ubiquitination | Uniprot | |
S191 | Phosphorylation | Uniprot | |
K227 | Acetylation | Uniprot | |
K227 | Ubiquitination | Uniprot | |
T235 | Phosphorylation | Uniprot | |
T238 | Phosphorylation | Uniprot | |
K245 | Acetylation | Uniprot | |
K245 | Ubiquitination | Uniprot | |
K246 | Ubiquitination | Uniprot | |
K367 | Acetylation | Uniprot | |
K367 | Ubiquitination | Uniprot | |
Y377 | Phosphorylation | Uniprot | |
K389 | Ubiquitination | Uniprot | |
T394 | Phosphorylation | Uniprot | |
T397 | Phosphorylation | Uniprot | |
S403 | Phosphorylation | Uniprot | |
T411 | Phosphorylation | Uniprot | |
S412 | Phosphorylation | Uniprot | |
Y430 | Phosphorylation | Uniprot | |
K450 | Acetylation | Uniprot | |
K450 | Ubiquitination | Uniprot | |
K453 | Ubiquitination | Uniprot | |
K456 | Acetylation | Uniprot | |
K456 | Ubiquitination | Uniprot | |
T473 | Phosphorylation | Uniprot | |
C491 | S-Nitrosylation | Uniprot | |
K500 | Ubiquitination | Uniprot | |
K506 | Acetylation | Uniprot | |
K507 | Ubiquitination | Uniprot | |
Y510 | Phosphorylation | Uniprot | |
Y556 | Phosphorylation | Uniprot | |
K619 | Methylation | Uniprot | |
K619 | Ubiquitination | Uniprot | |
Y634 | Phosphorylation | Uniprot | |
K637 | Acetylation | Uniprot | |
K637 | Ubiquitination | Uniprot | |
S672 | Phosphorylation | Uniprot | |
C736 | S-Nitrosylation | Uniprot | |
K737 | Ubiquitination | Uniprot | |
K742 | Acetylation | Uniprot | |
K742 | Ubiquitination | Uniprot | |
S751 | Phosphorylation | Uniprot | |
K760 | Ubiquitination | Uniprot | |
K764 | Acetylation | Uniprot | |
K764 | Ubiquitination | Uniprot | |
K798 | Ubiquitination | Uniprot | |
Y799 | Phosphorylation | Uniprot | |
Y803 | Phosphorylation | Uniprot | |
K806 | Ubiquitination | Uniprot | |
S825 | Phosphorylation | Uniprot | |
S841 | Phosphorylation | Uniprot | |
T842 | Phosphorylation | Uniprot | |
K851 | Ubiquitination | Uniprot | |
K856 | Acetylation | Uniprot | |
K856 | Ubiquitination | Uniprot | |
K881 | Ubiquitination | Uniprot | |
Y883 | Phosphorylation | Uniprot | |
Y899 | Phosphorylation | Uniprot | |
Y900 | Phosphorylation | Uniprot | |
S902 | Phosphorylation | Uniprot | |
R903 | Methylation | Uniprot | |
K907 | Acetylation | Uniprot | |
K907 | Ubiquitination | Uniprot | |
K911 | Ubiquitination | Uniprot | |
C918 | S-Nitrosylation | Uniprot | |
C926 | S-Nitrosylation | Uniprot | |
C934 | S-Nitrosylation | Uniprot | |
S938 | Phosphorylation | Uniprot | |
K941 | Ubiquitination | Uniprot | |
S1016 | Phosphorylation | Uniprot | |
S1019 | Phosphorylation | Uniprot | |
K1034 | Ubiquitination | Uniprot | |
K1074 | Ubiquitination | Uniprot | |
Y1096 | Phosphorylation | Uniprot | |
C1102 | S-Nitrosylation | Uniprot | |
S1109 | Phosphorylation | Uniprot | |
K1118 | Ubiquitination | Uniprot | |
K1122 | Ubiquitination | Uniprot | |
K1130 | Ubiquitination | Uniprot | |
Y1137 | Phosphorylation | Uniprot | |
K1155 | Acetylation | Uniprot | |
S1167 | Phosphorylation | Uniprot | |
K1179 | Ubiquitination | Uniprot | |
T1180 | Phosphorylation | Uniprot | |
Y1206 | Phosphorylation | Uniprot | |
K1209 | Acetylation | Uniprot | |
K1209 | Ubiquitination | Uniprot | |
Y1211 | Phosphorylation | Uniprot | |
K1215 | Ubiquitination | Uniprot | |
Y1218 | Phosphorylation | Uniprot | |
S1222 | Phosphorylation | Uniprot | |
R1226 | Methylation | Uniprot | |
S1229 | Phosphorylation | Uniprot | |
T1230 | Phosphorylation | Uniprot | |
Y1237 | Phosphorylation | Uniprot | |
K1254 | Ubiquitination | Uniprot | |
K1264 | Acetylation | Uniprot | |
K1264 | Ubiquitination | Uniprot | |
T1318 | Phosphorylation | Uniprot | |
S1341 | Phosphorylation | Uniprot | |
K1347 | Ubiquitination | Uniprot | |
K1387 | Ubiquitination | Uniprot | |
K1392 | Ubiquitination | Uniprot | |
T1396 | Phosphorylation | Uniprot | |
K1397 | Ubiquitination | Uniprot | |
Y1404 | Phosphorylation | Uniprot | |
Y1405 | Phosphorylation | Uniprot | |
Y1438 | Phosphorylation | Uniprot | |
S1440 | Phosphorylation | Uniprot | |
K1441 | Acetylation | Uniprot | |
K1441 | Methylation | Uniprot | |
K1441 | Ubiquitination | Uniprot | |
K1443 | Ubiquitination | Uniprot | |
K1449 | Acetylation | Uniprot | |
K1449 | Ubiquitination | Uniprot | |
K1461 | Ubiquitination | Uniprot | |
S1462 | Phosphorylation | Uniprot | |
S1466 | Phosphorylation | Uniprot | |
Y1477 | Phosphorylation | P12931 (SRC) | Uniprot |
T1482 | Phosphorylation | Uniprot | |
Y1487 | Phosphorylation | Uniprot | |
S1494 | Phosphorylation | Uniprot | |
K1501 | Acetylation | Uniprot | |
K1501 | Ubiquitination | Uniprot | |
Y1513 | Phosphorylation | Uniprot | |
K1516 | Acetylation | Uniprot | |
K1522 | Ubiquitination | Uniprot | |
S1524 | Phosphorylation | Uniprot | |
K1529 | Ubiquitination | Uniprot | |
K1535 | Acetylation | Uniprot | |
K1535 | Ubiquitination | Uniprot | |
S1544 | Phosphorylation | Uniprot | |
T1608 | Phosphorylation | Uniprot | |
K1609 | Ubiquitination | Uniprot | |
K1612 | Ubiquitination | Uniprot | |
S1618 | Phosphorylation | Uniprot | |
K1621 | Acetylation | Uniprot | |
Y1634 | Phosphorylation | Uniprot | |
Y1657 | Phosphorylation | Uniprot | |
Y1659 | Phosphorylation | Uniprot | |
T1660 | Phosphorylation | Uniprot | |
Y1664 | Phosphorylation | Uniprot | |
Y1673 | Phosphorylation | Uniprot | |
S1674 | Phosphorylation | Uniprot |
Research Backgrounds
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. Plays a role in early autophagosome formation.
Cytoplasmic vesicle membrane>Peripheral membrane protein>Cytoplasmic side. Membrane>Coated pit>Peripheral membrane protein>Cytoplasmic side. Melanosome. Cytoplasm>Cytoskeleton>Spindle.
Note: Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In complex with TACC3 and CKAP5 (forming the TACC3/ch-TOG/clathrin complex) localized to inter-microtubule bridges in mitotic spindles.
Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat. In the presence of light chains, hub assembly is influenced by both the pH and the concentration of calcium. Interacts with HIP1. Interacts with DENND1A, DENND1B and DENND1C (By similarity). May interact with OCRL (By similarity). Interacts with ERBB2. Interacts with FKBP6. Interacts with CKAP5 and TACC3 forming the TACC3/ch-TOG/clathrin complex located at spindle inter-microtubules bridges; the complex implicates clathrin triskelions; TACC3 and CLTC are proposed to form a composite microtubule interaction surface. Interacts with ATG16L1 (via N-terminus). Interacts with RFTN1; the interaction occurs in response to pathogens. Interacts with USP2 isoform 4.
The N-terminal seven-bladed beta-propeller is formed by WD40-like repeats, and projects inward from the polyhedral outer clathrin coat. It constitutes a major protein-protein interaction node.
Belongs to the clathrin heavy chain family.
Research Fields
· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Human Diseases > Neurodegenerative diseases > Huntington's disease.
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
· Organismal Systems > Nervous system > Synaptic vesicle cycle.
· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.
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