Neurofilament heavy polypeptide Antibody - #DF13211

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Product: Neurofilament heavy polypeptide Antibody
Catalog: DF13211
Description: Rabbit polyclonal antibody to Neurofilament heavy polypeptide
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 112kDa, 150 kDa; 112kD(Calculated).
Uniprot: P12036
RRID: AB_2846230

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Sheep(100%), Rabbit(88%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Neurofilament heavy polypeptide Antibody detects endogenous levels of total Neurofilament heavy polypeptide.
RRID:
AB_2846230
Cite Format: Affinity Biosciences Cat# DF13211, RRID:AB_2846230.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

200 kDa neurofilament protein; CMT2CC; Nefh; Neurofilament heavy polypeptide 200kDa; Neurofilament heavy polypeptide; Neurofilament triplet H protein; NF H; NF-H; NFH; NFH_HUMAN;

Immunogens

Immunogen:
Uniprot:

P12036(NFH_HUMAN) >>Visit HPA database.

Gene(ID):
NEFH(4744)
Sequence:
MMSFGGADALLGAPFAPLHGGGSLHYALARKGGAGGTRSAAGSSSGFHSWTRTSVSSVSASPSRFRGAGAASSTDSLDTLSNGPEGCMVAVATSRSEKEQLQALNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRSAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAAARALARFAQEAEAARVDLQKKAQALQEECGYLRRHHQEEVGELLGQIQGSGAAQAQMQAETRDALKCDVTSALREIRAQLEGHAVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEITEYRRQLQARTTELEALKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPIPFSLPEGLPKIPSVSTHIKVKSEEKIKVVEKSEKETVIVEEQTEETQVTEEVTEEEEKEAKEEEGKEEEGGEEEEAEGGEEETKSPPAEEAASPEKEAKSPVKEEAKSPAEAKSPEKEEAKSPAEVKSPEKAKSPAKEEAKSPPEAKSPEKEEAKSPAEVKSPEKAKSPAKEEAKSPAEAKSPEKAKSPVKEEAKSPAEAKSPVKEEAKSPAEVKSPEKAKSPTKEEAKSPEKAKSPEKAKSPEKEEAKSPEKAKSPVKAEAKSPEKAKSPVKAEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKTPEKAKSPVKEEAKSPEKAKSPEKAKTLDVKSPEAKTPAKEEARSPADKFPEKAKSPVKEEVKSPEKAKSPLKEDAKAPEKEIPKKEEVKSPVKEEEKPQEVKVKEPPKKAEEEKAPATPKTEEKKDSKKEEAPKKEAPKPKVEEKKEPAVEKPKESKVEAKKEEAEDKKKVPTPEKEAPAKVEVKEDAKPKEKTEVAKKEPDDAKAKEPSKPAEKKEAAPEKKDTKEEKAKKPEEKPKTEAKAKEDDKTLSKEPSKPKAEKAEKSSSTDQKDSKPPEKATEDKAAKGK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Rabbit
88
Pig
0
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P12036 As Substrate

Site PTM Type Enzyme
K31 Ubiquitination
S54 Phosphorylation
S59 Phosphorylation
S61 Phosphorylation
S63 Phosphorylation
Y111 Phosphorylation
K336 Ubiquitination
S337 Phosphorylation
Y385 Phosphorylation
R412 Methylation
S431 Phosphorylation
T501 Phosphorylation
S503 Phosphorylation P49841 (GSK3B)
S511 Phosphorylation
S518 Phosphorylation P28482 (MAPK1)
S526 Phosphorylation P28482 (MAPK1)
K531 Ubiquitination
S532 Phosphorylation P28482 (MAPK1)
S540 Phosphorylation
S546 Phosphorylation
S552 Phosphorylation
S560 Phosphorylation
K565 Ubiquitination
S566 Phosphorylation
S574 Phosphorylation
S580 Phosphorylation
S594 Phosphorylation
S600 Phosphorylation
S606 Phosphorylation
S614 Phosphorylation
S620 Phosphorylation
S628 Phosphorylation
S634 Phosphorylation
S640 Phosphorylation
S648 Phosphorylation
S654 Phosphorylation
K657 Acetylation
K659 Acetylation
K659 Ubiquitination
S660 Phosphorylation
K663 Acetylation
S668 Phosphorylation
S674 Phosphorylation
S682 Phosphorylation
S688 Phosphorylation
S696 Phosphorylation
S702 Phosphorylation
S710 Phosphorylation
S716 Phosphorylation
S724 Phosphorylation
S730 Phosphorylation
T738 Phosphorylation
S744 Phosphorylation
S752 Phosphorylation
S758 Phosphorylation
S769 Phosphorylation
S782 Phosphorylation
S793 Phosphorylation
S801 Phosphorylation
S807 Phosphorylation
S828 Phosphorylation
K895 Ubiquitination
K907 Acetylation
K908 Acetylation
T911 Phosphorylation
S948 Phosphorylation
S993 Phosphorylation

Research Backgrounds

Function:

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.

PTMs:

There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.

Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.

Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the intermediate filament family.

Research Fields

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

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