ODAM Antibody - #DF13204

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Product: ODAM Antibody
Catalog: DF13204
Description: Rabbit polyclonal antibody to ODAM
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Horse, Rabbit, Dog
Mol.Wt.: 17 kDa; 31kD(Calculated).
Uniprot: A1E959
RRID: AB_2846164

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Horse(90%), Rabbit(80%), Dog(90%)
Clonality:
Polyclonal
Specificity:
ODAM Antibody detects endogenous levels of total ODAM.
RRID:
AB_2846164
Cite Format: Affinity Biosciences Cat# DF13204, RRID:AB_2846164.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:
Uniprot:

A1E959(ODAM_HUMAN) >>Visit HPA database.

Gene(ID):
ODAM(54959)
Expression:
A1E959 ODAM_HUMAN:

Expressed in the junctional epithelium of healthy teeth. In periodontitis, absent in the pocket epithelium of the diseased periodontium but is detected in the gingival crevicular fluid.

Sequence:
MKIIILLGFLGATLSAPLIPQRLMSASNSNELLLNLNNGQLLPLQLQGPLNSWIPPFSGILQQQQQAQIPGLSQFSLSALDQFAGLLPNQIPLTGEASFAQGAQAGQVDPLQLQTPPQTQPGPSHVMPYVFSFKMPQEQGQMFQYYPVYMVLPWEQPQQTVPRSPQQTRQQQYEEQIPFYAQFGYIPQLAEPAISGGQQQLAFDPQLGTAPEIAVMSTGEEIPYLQKEAINFRHDSAGVFMPSTSPKPSTTNVFTSAVDQTITPELPEEKDKTDSLREP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
90
Dog
90
Rabbit
80
Bovine
78
Sheep
67
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - A1E959 As Substrate

Site PTM Type Enzyme
T255 Phosphorylation
S256 Phosphorylation

Research Backgrounds

Function:

Tooth-associated epithelia protein that probably plays a role in odontogenesis, the complex process that results in the initiation and generation of the tooth. May be incorporated in the enamel matrix at the end of mineralization process. Involved in the induction of RHOA activity via interaction with ARHGEF and expression of downstream factors such as ROCK. Plays a role in attachment of the junctional epithelium to the tooth surface.

PTMs:

O-glycosylated.

Subcellular Location:

Secreted. Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the junctional epithelium of healthy teeth. In periodontitis, absent in the pocket epithelium of the diseased periodontium but is detected in the gingival crevicular fluid.

Subunit Structure:

Interacts (via C-terminus) with ARHGEF5.

Family&Domains:

Belongs to the ODAM family.

References

1). Single-cell transcriptomics reveals cell atlas and identifies cycling tumor cells responsible for recurrence in ameloblastoma. International journal of oral science, 2024 (PubMed: 38424060) [IF=14.9]

Application: IF/ICC    Species: Human    Sample:

Single-cell transcriptomic profiling of the multicellular ecosystem of ameloblastoma. a Workflow showing the collection and processing of fresh patient samples from 11 tumors for scRNA-seq. (b) UMAP plot of 92,622 single cells from scRNA-seq labeled by cell type with different colors. c UMAP plot showing the expression of selected marker genes in the 9 clusters. (d) Representative images of multiplex immunohistochemistry showing the expression of PCK, CD3, CD34, CD68, ODAM, FAP in ameloblastoma; scale bar, 100 μm, and 20 μm
2). A novel junctional epithelial cell line, mHAT-JE01, derived from incisor epithelial cells. Journal of Oral Biosciences, 2023 (PubMed: 36693475) [IF=2.4]

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