OBFC2B Antibody - #DF13202
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13202, RRID:AB_2846162.
Fold/Unfold
2610036N15Rik; hSSB1; LP3587; MGC125161; MGC2731; MGC30353; Nabp2; Nucleic acid binding protein 2; OBFC2B; Oligonucleotide/oligosaccharide binding fold containing 2B; Oligonucleotide/oligosaccharide binding fold containing protein 2B; RGD1308158; Sensor of single strand DNA complex subunit B1; Sensor of ssDNA subunit B1; Single stranded DNA binding protein 1; SOSS complex subunit B1; SSB1;
Immunogens
- Q9BQ15 SOSB1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTTETFVKDIKPGLKNLNLIFIVLETGRVTKTKDGHEVRTCKVADKTGSINISVWDDVGNLIQPGDIIRLTKGYASVFKGCLTLYTGRGGDLQKIGEFCMVYSEVPNFSEPNPEYSTQQAPNKAVQNDSNPSASQPTTGPSAASPASENQNGNGLSAPPGPGGGPHPPHTPSHPPSTRITRSQPNHTPAGPPGPSSNPVSNGKETRRSSKR
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9BQ15 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K8 | Ubiquitination | Uniprot | |
| K15 | Acetylation | Uniprot | |
| Y74 | Phosphorylation | Uniprot | |
| S116 | Phosphorylation | Uniprot | |
| T117 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S134 | Phosphorylation | Uniprot | |
| T187 | Phosphorylation | Uniprot | |
| S195 | Phosphorylation | Uniprot | |
| S200 | Phosphorylation | Uniprot | |
| K203 | Acetylation | Uniprot |
Research Backgrounds
Component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. In the SOSS complex, acts as a sensor of single-stranded DNA that binds to single-stranded DNA, in particular to polypyrimidines. The SOSS complex associates with DNA lesions and influences diverse endpoints in the cellular DNA damage response including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. Required for efficient homologous recombination-dependent repair of double-strand breaks (DSBs) and ATM-dependent signaling pathways.
Phosphorylated by ATM in response to DNA damage. Phosphorylation prevents degradation by the proteasome, hence stabilization of the protein and accumulation within cells.
Nucleus.
Note: Localizes to nuclear foci following DNA damage. Foci formation is not cell-cycle dependent. Partial colocalization with RAD51 after ionizing radiation treatment.
Component of the SOSS complex, composed of SOSS-B (SOSS-B1/NABP2 or SOSS-B2/NABP1), SOSS-A/INTS3 and SOSS-C/INIP. SOSS complexes containing SOSS-B1/NABP2 are more abundant than complexes containing SOSS-B2/NABP1. Directly interacts with ATM, SOSS-A/INTS3 and RAD51. Interacts with INTS7.
Belongs to the SOSS-B family. SOSS-B1 subfamily.
References
Application: WB Species: Human Sample: LM3 and Huh7 cell
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