NXF2 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Mouse testis tissue, using NXF2 Antibody.

Product:	NXF2 Antibody
Catalog:	DF13201
Description:	Rabbit polyclonal antibody to NXF2
Application:	WB
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	72 kDa; 72kD(Calculated).
Uniprot:	Q9GZY0
RRID:	AB_2846161

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

NXF2 Antibody detects endogenous levels of total NXF2.

RRID:

AB_2846161
Cite Format: Affinity Biosciences Cat# DF13201, RRID:AB_2846161.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

cancer/testis antigen 39; CT39; FLJ20416; Nuclear RNA export factor 2; NXF2B; T complex protein 11 homolog; TAP like protein 2; TAPL-2; TAPL2; TCP11X2;

Immunogens

Immunogen:

Uniprot:

Q9GZY0(NXF2_HUMAN) >>Visit HPA database.

Gene(ID):

NXF2; NXF2B(56001)

Expression:

Q9GZY0 NXF2_HUMAN:

Expressed almost exclusively in testis. Also expressed in several cancers.

Sequence:

Q9GZY0 NXF2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MCSTLKKCGTYRTEVAECHDHGSTFQGRKKGGSSFRDNFDKRSCHYEHGGYERPPSHCQENDGSVEMRDVHKDQQLRHTPYSIRCERRMKWHSEDEIRITTWRNRKPPERKMSQNTQDGYTRNWFKVTIPYGIKYDKAWLMNSIQSHCSDRFTPVDFHYVRNRACFFVQDASAASALKDVSYKIYDDENQKICIFVNHSTAPYSVKNKLKPGQMEMLKLTMNKRYNVSQQALDLQNLRFDPDLMGRDIDIILNRRNCMAATLKIIERNFPELLSLNLCNNKLYQLDGLSDITEKAPKVKTLNLSKNKLESAWELGKVKGLKLEELWLEGNPLCSTFSDQSAYVSAIRDCFPKLLRLDGRELSAPVIVDIDSSETMKPCKENFTGSETLKHLVLQFLQQYYSIYDSGDRQGLLGAYHDEACFSLAIPFDPKDSAPSSLCKYFEDSRNMKTLKDPYLKGELLRRTKRDIVDSLSALPKTQHDLSSILVDVWCQTERMLCFSVNGVFKEVEGQSQGSVLAFTRTFIATPGSSSSLCIVNDELFVRDASPQETQSAFSIPVSTLSSSSEPSLSQEQQEMVQAFSAQSGMKLEWSQKCLQDNEWNYTRAGQAFTMLQTEGKIPAEAFKQIS

PTMs - Q9GZY0 As Substrate

Q9GZY0

Site	PTM Type	Source
T13	Phosphorylation	Uniprot
S34	Phosphorylation	Uniprot
S82	Phosphorylation	Uniprot
K90	Methylation	Uniprot
Y120	Phosphorylation	Uniprot
Y185	Phosphorylation	Uniprot
Y203	Phosphorylation	Uniprot
T383	Phosphorylation	Uniprot

Research Backgrounds

Q9GZY0_NXF2_HUMAN

Function:

Involved in the export of mRNA from the nucleus to the cytoplasm.

Subcellular Location:

Nucleus>Nucleoplasm. Cytoplasm.
Note: Localized in the nucleoplasm and at the nuclear envelope. Shuttles between the nucleus and the cytoplasm.

Tissue Specificity:

Expressed almost exclusively in testis. Also expressed in several cancers.

Subunit Structure:

Interacts with NXT1, NXT2, E1B-AP5, the REF proteins and with nucleoporins, Nup62, Nup153 and Nup214. Interacts with LUZP4.

Family&Domains:

The NTF2 domain heterodimerizes with NXT1 and NXT2. The formation of NXF1/NXT1 heterodimers is required for NXF2-mediated nuclear mRNA export.

The leucine-rich repeats and the NTF2-domain are essential for the export of mRNA from the nucleus.

The C-terminal fragment, containing the TAP domain (also called UBA-like domain) and part of the NTF2-like domain, named the NPC-binding domain, mediates direct interactions with nucleoporin-FG-repeats and is necessary and sufficient for localization of NXF2 to the nuclear rim.

The RNA-binding domain is a non-canonical RNP-type domain.

Belongs to the NXF family.

Research Fields

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

· Genetic Information Processing > Translation > RNA transport.

· Genetic Information Processing > Translation > mRNA surveillance pathway.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

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NXF2 Antibody - #DF13201