NUS1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Mouse brain tissue, using NUS1 Antibody.

Product:	NUS1 Antibody
Catalog:	DF13200
Description:	Rabbit polyclonal antibody to NUS1
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	31 kDa; 33kD(Calculated).
Uniprot:	Q96E22
RRID:	AB_2846160

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(91%), Horse(100%), Sheep(100%), Rabbit(91%), Dog(100%)

Clonality:

Polyclonal

Specificity:

NUS1 Antibody detects endogenous levels of total NUS1.

RRID:

AB_2846160
Cite Format: Affinity Biosciences Cat# DF13200, RRID:AB_2846160.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:

Uniprot:

Q96E22(NGBR_HUMAN) >>Visit HPA database.

Gene(ID):

NUS1(116150)

Sequence:

Q96E22 NGBR_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTGLYELVWRVLHALLCLHRTLTSWLRVRFGTWNWIWRRCCRAASAAVLAPLGFTLRKPPAVGRNRRHHRHPRGGSCLAAAHHRMRWRADGRSLEKLPVHMGLVITEVEQEPSFSDIASLVVWCMAVGISYISVYDHQGIFKRNNSRLMDEILKQQQELLGLDCSKYSPEFANSNDKDDQVLNCHLAVKVLSPEDGKADIVRAAQDFCQLVAQKQKRPTDLDVDTLASLLSSNGCPDPDLVLKFGPVDSTLGFLPWHIRLTEIVSLPSHLNISYEDFFSALRQYAACEQRLGK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Sheep

100

Dog

100

Pig

Rabbit

Chicken

Bovine

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96E22 As Substrate

Q96E22

Site	PTM Type	Source
S45	Phosphorylation	Uniprot
S76	Phosphorylation	Uniprot
N144	N-Glycosylation	Uniprot
K154	Ubiquitination	Uniprot
K166	Ubiquitination	Uniprot
S168	Phosphorylation	Uniprot
K177	Ubiquitination	Uniprot
K189	Ubiquitination	Uniprot
K197	Ubiquitination	Uniprot
K214	Ubiquitination	Uniprot
N271	N-Glycosylation	Uniprot

Research Backgrounds

Q96E22_NGBR_HUMAN

Function:

With DHDDS, forms the dehydrodolichyl diphosphate synthase (DDS) complex, an essential component of the dolichol monophosphate (Dol-P) biosynthetic machinery. Both subunits contribute to enzymatic activity, i.e. condensation of multiple copies of isopentenyl pyrophosphate (IPP) to farnesyl pyrophosphate (FPP) to produce dehydrodolichyl diphosphate (Dedol-PP), a precursor of dolichol phosphate which is utilized as a sugar carrier in protein glycosylation in the endoplasmic reticulum (ER). Regulates the glycosylation and stability of nascent NPC2, thereby promoting trafficking of LDL-derived cholesterol. Acts as a specific receptor for the N-terminus of Nogo-B, a neural and cardiovascular regulator.

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein.
Note: Colocalizes with Nogo-B during VEGF and wound healing angiogenesis.

Subunit Structure:

Forms an active dehydrodolichyl diphosphate synthase complex with DHDDS. Interacts with NPC2.

Family&Domains:

Belongs to the UPP synthase family.

Research Fields

· Metabolism > Metabolism of terpenoids and polyketides > Terpenoid backbone biosynthesis.

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NUS1 Antibody - #DF13200