NUDT9 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from Mouse brain tissue, using NUDT9 Antibody.

Product:	NUDT9 Antibody
Catalog:	DF13199
Description:	Rabbit polyclonal antibody to NUDT9
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	39 kDa; 39kD(Calculated).
Uniprot:	Q9BW91
RRID:	AB_2846159

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(92%), Dog(100%)

Clonality:

Polyclonal

Specificity:

NUDT9 Antibody detects endogenous levels of total NUDT9.

RRID:

AB_2846159
Cite Format: Affinity Biosciences Cat# DF13199, RRID:AB_2846159.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Adenosine diphosphoribose pyrophosphatase; ADP ribose diphosphatase; ADP ribose phosphohydrolase; ADP ribose pyrophosphatase, mitochondrial precursor; ADP ribose pyrosphosphatase NUDT9; ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ADP-ribose pyrophosphatase, mitochondrial; ADPR PPase; ADPR-PPase; EC 3.6.1.13; Nucleoside diphosphate linked moiety X motif 9; Nucleoside diphosphate linked moiety X type motif 9; Nucleoside diphosphate-linked moiety X motif 9; Nudix (nucleoside diphosphate linked moiety X) type motif 9; Nudix hydrolase 9; Nudix motif 9; NUDT10; NUDT9; NUDT9_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q9BW91(NUDT9_HUMAN) >>Visit HPA database.

Gene(ID):

NUDT9(53343)

Expression:

Q9BW91 NUDT9_HUMAN:

Ubiquitously expressed but isoform 1 is the most predominant isoform.

Sequence:

Q9BW91 NUDT9_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAGRLLGKALAAVSLSLALASVTIRSSRCRGIQAFRNSFSSSWFHLNTNVMSGSNGSKENSHNKARTSPYPGSKVERSQVPNEKVGWLVEWQDYKPVEYTAVSVLAGPRWADPQISESNFSPKFNEKDGHVERKSKNGLYEIENGRPRNPAGRTGLVGRGLLGRWGPNHAADPIITRWKRDSSGNKIMHPVSGKHILQFVAIKRKDCGEWAIPGGMVDPGEKISATLKREFGEEALNSLQKTSAEKREIEEKLHKLFSQDHLVIYKGYVDDPRNTDNAWMETEAVNYHDETGEIMDNLMLEAGDDAGKVKWVDINDKLKLYASHSQFIKLVAEKRDAHWSEDSEADCHAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Dog

100

Horse

Bovine

Sheep

Rabbit

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BW91 As Substrate

Q9BW91

Site	PTM Type	Source
S68	Phosphorylation	Uniprot
Y70	Phosphorylation	Uniprot
S73	Phosphorylation	Uniprot
K74	Acetylation	Uniprot
S121	Phosphorylation	Uniprot
Y140	Phosphorylation	Uniprot
S183	Phosphorylation	Uniprot
S258	Phosphorylation	Uniprot
K319	Acetylation	Uniprot
Y321	Phosphorylation	Uniprot
S323	Phosphorylation	Uniprot

Research Backgrounds

Q9BW91_NUDT9_HUMAN

Function:

Hydrolyzes ADP-ribose (ADPR) to AMP and ribose 5'-phosphate.

Subcellular Location:

Mitochondrion.

Tissue Specificity:

Ubiquitously expressed but isoform 1 is the most predominant isoform.

Subunit Structure:

Monomer. Interacts with GLOD4.

Family&Domains:

Belongs to the Nudix hydrolase family. NudF subfamily.

Research Fields

· Metabolism > Nucleotide metabolism > Purine metabolism.

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For Research Use Only.
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NUDT9 Antibody - #DF13199