NPLOC4 Antibody - #DF13194
| Product: | NPLOC4 Antibody |
| Catalog: | DF13194 |
| Description: | Rabbit polyclonal antibody to NPLOC4 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 68 kDa; 68kD(Calculated). |
| Uniprot: | Q8TAT6 |
| RRID: | AB_2846154 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13194, RRID:AB_2846154.
Fold/Unfold
KIAA1499; NPL4; NPL4, S. cerevisiae, homolog of; NPL4_HUMAN; nploc4; nuclear protein localization 4 homolog (S. cerevisiae); Nuclear protein localization 4 homolog; Nuclear protein localization protein 4 homolog; Nuclear protein localization protein 4, S. cerevisiae, homolog of; Protein NPL4;
Immunogens
Expressed at highest levels in brain, heart, skeletal muscle, kidney and fetal liver.
- Q8TAT6 NPL4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAESIIIRVQSPDGVKRITATKRETAATFLKKVAKEFGFQNNGFSVYINRNKTGEITASSNKSLNLLKIKHGDLLFLFPSSLAGPSSEMETSVPPGFKVFGAPNVVEDEIDQYLSKQDGKIYRSRDPQLCRHGPLGKCVHCVPLEPFDEDYLNHLEPPVKHMSFHAYIRKLTGGADKGKFVALENISCKIKSGCEGHLPWPNGICTKCQPSAITLNRQKYRHVDNIMFENHTVADRFLDFWRKTGNQHFGYLYGRYTEHKDIPLGIRAEVAAIYEPPQIGTQNSLELLEDPKAEVVDEIAAKLGLRKVGWIFTDLVSEDTRKGTVRYSRNKDTYFLSSEECITAGDFQNKHPNMCRLSPDGHFGSKFVTAVATGGPDNQVHFEGYQVSNQCMALVRDECLLPCKDAPELGYAKESSSEQYVPDVFYKDVDKFGNEITQLARPLPVEYLIIDITTTFPKDPVYTFSISQNPFPIENRDVLGETQDFHSLATYLSQNTSSVFLDTISDFHLLLFLVTNEVMPLQDSISLLLEAVRTRNEELAQTWKRSEQWATIEQLCSTVGGQLPGLHEYGAVGGSTHTATAAMWACQHCTFMNQPGTGHCEMCSLPRT
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8TAT6 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S4 | Phosphorylation | Uniprot | |
| S11 | Phosphorylation | Uniprot | |
| K16 | Ubiquitination | Uniprot | |
| T21 | Phosphorylation | Uniprot | |
| T28 | Phosphorylation | Uniprot | |
| K31 | Ubiquitination | Uniprot | |
| K35 | Ubiquitination | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| S60 | Phosphorylation | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| S63 | Phosphorylation | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| Y113 | Phosphorylation | Uniprot | |
| K116 | Ubiquitination | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| K137 | Ubiquitination | Uniprot | |
| Y151 | Phosphorylation | Uniprot | |
| T172 | Phosphorylation | Uniprot | |
| K177 | Ubiquitination | Uniprot | |
| C188 | S-Nitrosylation | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| K243 | Ubiquitination | Uniprot | |
| Y251 | Phosphorylation | Uniprot | |
| K260 | Ubiquitination | Uniprot | |
| Y274 | Phosphorylation | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| K302 | Ubiquitination | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| K350 | Ubiquitination | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| K404 | Ubiquitination | Uniprot | |
| K413 | Ubiquitination | Uniprot | |
| K427 | Ubiquitination | Uniprot | |
| K431 | Ubiquitination | Uniprot | |
| K544 | Ubiquitination | Uniprot |
Research Backgrounds
The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). Acts as a negative regulator of type I interferon production via the complex formed with VCP and UFD1, which binds to DDX58/RIG-I and recruits RNF125 to promote ubiquitination and degradation of DDX58/RIG-I.
Cytoplasm>Cytosol. Endoplasmic reticulum. Nucleus.
Note: Associated with the endoplasmic reticulum and nuclear.
Expressed at highest levels in brain, heart, skeletal muscle, kidney and fetal liver.
Heterodimer with UFD1. The heterodimer binds ubiquitinated proteins (By similarity). The heterodimer binds to VCP and inhibits Golgi membrane fusion. Interacts with ZFAND2B; probably through VCP.
Binds ubiquitinated proteins via its RanBP2-type zinc finger.
Belongs to the NPL4 family.
Research Fields
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.