NOXA Antibody - #DF13193

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Product: NOXA Antibody
Catalog: DF13193
Description: Rabbit polyclonal antibody to NOXA
Application: WB IF/ICC
Reactivity: Human
Mol.Wt.: 11 kDa; 6kD(Calculated).
Uniprot: Q13794
RRID: AB_2846153

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
NOXA Antibody detects endogenous levels of total NOXA.
RRID:
AB_2846153
Cite Format: Affinity Biosciences Cat# DF13193, RRID:AB_2846153.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Adult T cell leukemia derived PMA responsive; APR; APR_HUMAN; ATL-derived; Immediate early response protein APR; Immediate-early-response protein APR; NOXA; Phorbol 12 myristate 13 acetate induced protein 1; Phorbol-12-myristate-13-acetate-induced protein 1; PMA induced protein 1; PMA-induced protein 1; PMA-responsive gene; Pmaip1; Protein Noxa;

Immunogens

Immunogen:
Uniprot:

Q13794(APR_HUMAN) >>Visit HPA database.

Gene(ID):
PMAIP1(5366)
Expression:
Q13794 APR_HUMAN:

Highly expressed in adult T-cell leukemia cell line.

Sequence:
MPGKKARKNAQPSPARAPAELEVECATQLRRFGDKLNFRQKLLNLISKLFCSGT

PTMs - Q13794 As Substrate

Site PTM Type Enzyme
S13 Phosphorylation Q00535 (CDK5)
K35 Ubiquitination
K41 Ubiquitination
K48 Ubiquitination

Research Backgrounds

Function:

Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1 (By similarity). Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1.

Subcellular Location:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in adult T-cell leukemia cell line.

Subunit Structure:

Interacts with MCL1, BCL2A1 and BAX.

Family&Domains:

The BH3 motif is essential for pro-apoptotic activity.

Belongs to the PMAIP1 family.

Research Fields

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis - multiple species.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

Restrictive clause

 

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For Research Use Only.
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