NNMT Antibody | Affinity Biosciences

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Western blot analysis of extracts from HepG2 cells, using NNMT Antibody.

Product:	NNMT Antibody
Catalog:	DF13190
Description:	Rabbit polyclonal antibody to NNMT
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Dog
Mol.Wt.:	30 kDa; 30kD(Calculated).
Uniprot:	P40261
RRID:	AB_2846150

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(83%), Dog(92%)

Clonality:

Polyclonal

Specificity:

NNMT Antibody detects endogenous levels of total NNMT.

RRID:

AB_2846150
Cite Format: Affinity Biosciences Cat# DF13190, RRID:AB_2846150.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

EC 2.1.1.1; Nicotinamide N methyltransferase; Nicotinamide N-methyltransferase; NNMT; NNMT_HUMAN;

Immunogens

Immunogen:

Uniprot:

P40261(NNMT_HUMAN) >>Visit HPA database.

Gene(ID):

NNMT(4837)

Expression:

P40261 NNMT_HUMAN:

Predominantly expressed in the liver. A lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. Not detected in the brain or pancreas.

Sequence:

P40261 NNMT_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MESGFTSKDTYLSHFNPRDYLEKYYKFGSRHSAESQILKHLLKNLFKIFCLDGVKGDLLIDIGSGPTIYQLLSACESFKEIVVTDYSDQNLQELEKWLKKEPEAFDWSPVVTYVCDLEGNRVKGPEKEEKLRQAVKQVLKCDVTQSQPLGAVPLPPADCVLSTLCLDAACPDLPTYCRALRNLGSLLKPGGFLVIMDALKSSYYMIGEQKFSSLPLGREAVEAAVKEAGYTIEWFEVISQSYSSTMANNEGLFSLVARKLSRPL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

Pig

Xenopus

Horse

Bovine

Sheep

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P40261 As Substrate

P40261

Site	PTM Type	Source
M1	Acetylation	Uniprot
S3	Phosphorylation	Uniprot
K8	Acetylation	Uniprot
K8	Methylation	Uniprot
K8	Ubiquitination	Uniprot
Y11	Phosphorylation	Uniprot
K23	Ubiquitination	Uniprot
Y24	Phosphorylation	Uniprot
Y25	Phosphorylation	Uniprot
K26	Ubiquitination	Uniprot
S35	Phosphorylation	Uniprot
K39	Acetylation	Uniprot
K39	Ubiquitination	Uniprot
K43	Acetylation	Uniprot
K43	Ubiquitination	Uniprot
K47	Ubiquitination	Uniprot
K96	Ubiquitination	Uniprot
K100	Ubiquitination	Uniprot
S108	Phosphorylation	Uniprot
Y113	Phosphorylation	Uniprot
K123	Ubiquitination	Uniprot
K136	Ubiquitination	Uniprot
Y203	Phosphorylation	Uniprot
Y204	Phosphorylation	Uniprot
K210	Ubiquitination	Uniprot

Research Backgrounds

P40261_NNMT_HUMAN

Function:

Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.

Subcellular Location:

Cytoplasm.

Tissue Specificity:

Predominantly expressed in the liver. A lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. Not detected in the brain or pancreas.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the class I-like SAM-binding methyltransferase superfamily. NNMT/PNMT/TEMT family.

Research Fields

· Metabolism > Metabolism of cofactors and vitamins > Nicotinate and nicotinamide metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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NNMT Antibody - #DF13190