Product: NNMT Antibody
Catalog: DF13190
Description: Rabbit polyclonal antibody to NNMT
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Dog
Mol.Wt.: 30 kDa; 30kD(Calculated).
Uniprot: P40261
RRID: AB_2846150

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(83%), Dog(92%)
Clonality:
Polyclonal
Specificity:
NNMT Antibody detects endogenous levels of total NNMT.
RRID:
AB_2846150
Cite Format: Affinity Biosciences Cat# DF13190, RRID:AB_2846150.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EC 2.1.1.1; Nicotinamide N methyltransferase; Nicotinamide N-methyltransferase; NNMT; NNMT_HUMAN;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P40261 NNMT_HUMAN:

Predominantly expressed in the liver. A lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. Not detected in the brain or pancreas.

Sequence:
MESGFTSKDTYLSHFNPRDYLEKYYKFGSRHSAESQILKHLLKNLFKIFCLDGVKGDLLIDIGSGPTIYQLLSACESFKEIVVTDYSDQNLQELEKWLKKEPEAFDWSPVVTYVCDLEGNRVKGPEKEEKLRQAVKQVLKCDVTQSQPLGAVPLPPADCVLSTLCLDAACPDLPTYCRALRNLGSLLKPGGFLVIMDALKSSYYMIGEQKFSSLPLGREAVEAAVKEAGYTIEWFEVISQSYSSTMANNEGLFSLVARKLSRPL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
92
Pig
83
Xenopus
45
Horse
0
Bovine
0
Sheep
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P40261 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S3 Phosphorylation
K8 Acetylation
K8 Methylation
K8 Ubiquitination
Y11 Phosphorylation
K23 Ubiquitination
Y24 Phosphorylation
Y25 Phosphorylation
K26 Ubiquitination
S35 Phosphorylation
K39 Acetylation
K39 Ubiquitination
K43 Acetylation
K43 Ubiquitination
K47 Ubiquitination
K96 Ubiquitination
K100 Ubiquitination
S108 Phosphorylation
Y113 Phosphorylation
K123 Ubiquitination
K136 Ubiquitination
Y203 Phosphorylation
Y204 Phosphorylation
K210 Ubiquitination

Research Backgrounds

Function:

Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Predominantly expressed in the liver. A lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. Not detected in the brain or pancreas.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the class I-like SAM-binding methyltransferase superfamily. NNMT/PNMT/TEMT family.

Research Fields

· Metabolism > Metabolism of cofactors and vitamins > Nicotinate and nicotinamide metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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