NIT2 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Mouse kidney tissue, using NIT2 Antibody.

Product:	NIT2 Antibody
Catalog:	DF13186
Description:	Rabbit polyclonal antibody to NIT2
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	30 kDa; 31kD(Calculated).
Uniprot:	Q9NQR4
RRID:	AB_2846146

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(91%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(91%)

Clonality:

Polyclonal

Specificity:

NIT2 Antibody detects endogenous levels of total NIT2.

RRID:

AB_2846146
Cite Format: Affinity Biosciences Cat# DF13186, RRID:AB_2846146.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CUA002; MGC111199; NIT 2; Nit protein 2; NIT2; NIT2_HUMAN; Nitrilase family member 2; Nitrilase homolog 2; Omega amidase NIT2; Omega-amidase NIT2;

Immunogens

Immunogen:

Uniprot:

Q9NQR4(NIT2_HUMAN) >>Visit HPA database.

Gene(ID):

NIT2(56954)

Expression:

Q9NQR4 NIT2_HUMAN:

Detected in fetal brain (at protein level). Ubiquitous. Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, prostate, spleen, thymus, prostate, testis, ovary, small intestine and colon.

Sequence:

Q9NQR4 NIT2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTSFRLALIQLQISSIKSDNVTRACSFIREAATQGAKIVSLPECFNSPYGAKYFPEYAEKIPGESTQKLSEVAKECSIYLIGGSIPEEDAGKLYNTCAVFGPDGTLLAKYRKIHLFDIDVPGKITFQESKTLSPGDSFSTFDTPYCRVGLGICYDMRFAELAQIYAQRGCQLLVYPGAFNLTTGPAHWELLQRSRAVDNQVYVATASPARDDKASYVAWGHSTVVNPWGEVLAKAGTEEAIVYSDIDLKKLAEIRQQIPVFRQKRSDLYAVEMKKP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Sheep

100

Rabbit

100

Bovine

Dog

Zebrafish

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NQR4 As Substrate

Q9NQR4

Site	PTM Type	Source
S26	Phosphorylation	Uniprot
K37	Ubiquitination	Uniprot
Y49	Phosphorylation	Uniprot
K52	Ubiquitination	Uniprot
K60	Ubiquitination	Uniprot
K68	Acetylation	Uniprot
K68	Ubiquitination	Uniprot
K92	Ubiquitination	Uniprot
K109	Ubiquitination	Uniprot
K112	Ubiquitination	Uniprot
K130	Ubiquitination	Uniprot
S133	Phosphorylation	Uniprot
Y145	Phosphorylation	Uniprot
C146	S-Nitrosylation	Uniprot
Y165	Phosphorylation	Uniprot
Y202	Phosphorylation	Uniprot
S207	Phosphorylation	Uniprot
T237	Phosphorylation	Uniprot
S244	Phosphorylation	Uniprot
K249	Acetylation	Uniprot
K249	Ubiquitination	Uniprot
K250	Acetylation	Uniprot
R265	Methylation	Uniprot
S266	Phosphorylation	Uniprot
Y269	Phosphorylation	Uniprot

Research Backgrounds

Q9NQR4_NIT2_HUMAN

Function:

Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.

Subcellular Location:

Cytoplasm.

Tissue Specificity:

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the carbon-nitrogen hydrolase superfamily. NIT1/NIT2 family.

Research Fields

· Metabolism > Amino acid metabolism > Alanine, aspartate and glutamate metabolism.

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NIT2 Antibody - #DF13186