MYH10 Antibody - #DF13164

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Product: MYH10 Antibody
Catalog: DF13164
Description: Rabbit polyclonal antibody to MYH10
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 229 kDa; 229kD(Calculated).
Uniprot: P35580
RRID: AB_2846124

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
MYH10 Antibody detects endogenous levels of total MYH10.
RRID:
AB_2846124
Cite Format: Affinity Biosciences Cat# DF13164, RRID:AB_2846124.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cellular myosin heavy chain; Cellular myosin heavy chain type B; Cellular myosin heavy chain type B type B; MGC134913; MGC134914; MYH 10; Myh10; MYH10_HUMAN; Myosin 10; Myosin heavy chain 10; Myosin heavy chain 10 non muscle; Myosin heavy chain; Myosin heavy chain non muscle 11b; Myosin heavy chain nonmuscle IIb; Myosin heavy chain nonmuscle type B; Myosin heavy polypeptide 10 non muscle; Myosin-10; Myosin10; NMMHC B; NMMHC II b; NMMHC II-b; NMMHC IIB; NMMHC-B; NMMHC-IIB; NMMHCB; Non muscle myosin heavy chain B; Non muscle myosin heavy chain IIB; Non muscle myosin II heavy chain B; non-muscle IIb; Non-muscle myosin heavy chain B; Non-muscle myosin heavy chain IIb; Nonmuscle myosin heavy chain B; Nonmuscle myosin heavy chain IIB; Nonmuscle myosin II heavy chain B; Nonmuscle myosin IIB; type B;

Immunogens

Immunogen:
Uniprot:

P35580(MYH10_HUMAN) >>Visit HPA database.

Gene(ID):
MYH10(4628)
Expression:
P35580 MYH10_HUMAN:

Isoform 1 is expressed in cerebellum and spinal chord. Isoform 2 is expressed in cerebrum and retina. Isoform 3 is expressed in the cerebrum and to a much lower extent in cerebellum.

Sequence:
MAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHGFEAASIKEERGDEVMVELAENGKKAMVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIEMYRGKKRHEMPPHIYAISESAYRCMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKDHNIPGELERQLLQANPILESFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAVRQAKDERTFHIFYQLLSGAGEHLKSDLLLEGFNNYRFLSNGYIPIPGQQDKDNFQETMEAMHIMGFSHEEILSMLKVVSSVLQFGNISFKKERNTDQASMPENTVAQKLCHLLGMNVMEFTRAILTPRIKVGRDYVQKAQTKEQADFAVEALAKATYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIDLIERPANPPGVLALLDEECWFPKATDKTFVEKLVQEQGSHSKFQKPRQLKDKADFCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSSDRFVAELWKDVDRIVGLDQVTGMTETAFGSAYKTKKGMFRTVGQLYKESLTKLMATLRNTNPNFVRCIIPNHEKRAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACERMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIIFFQAVCRGYLARKAFAKKQQQLSALKVLQRNCAAYLKLRHWQWWRVFTKVKPLLQVTRQEEELQAKDEELLKVKEKQTKVEGELEEMERKHQQLLEEKNILAEQLQAETELFAEAEEMRARLAAKKQELEEILHDLESRVEEEEERNQILQNEKKKMQAHIQDLEEQLDEEEGARQKLQLEKVTAEAKIKKMEEEILLLEDQNSKFIKEKKLMEDRIAECSSQLAEEEEKAKNLAKIRNKQEVMISDLEERLKKEEKTRQELEKAKRKLDGETTDLQDQIAELQAQIDELKLQLAKKEEELQGALARGDDETLHKNNALKVVRELQAQIAELQEDFESEKASRNKAEKQKRDLSEELEALKTELEDTLDTTAAQQELRTKREQEVAELKKALEEETKNHEAQIQDMRQRHATALEELSEQLEQAKRFKANLEKNKQGLETDNKELACEVKVLQQVKAESEHKRKKLDAQVQELHAKVSEGDRLRVELAEKASKLQNELDNVSTLLEEAEKKGIKFAKDAASLESQLQDTQELLQEETRQKLNLSSRIRQLEEEKNSLQEQQEEEEEARKNLEKQVLALQSQLADTKKKVDDDLGTIESLEEAKKKLLKDAEALSQRLEEKALAYDKLEKTKNRLQQELDDLTVDLDHQRQVASNLEKKQKKFDQLLAEEKSISARYAEERDRAEAEAREKETKALSLARALEEALEAKEEFERQNKQLRADMEDLMSSKDDVGKNVHELEKSKRALEQQVEEMRTQLEELEDELQATEDAKLRLEVNMQAMKAQFERDLQTRDEQNEEKKRLLIKQVRELEAELEDERKQRALAVASKKKMEIDLKDLEAQIEAANKARDEVIKQLRKLQAQMKDYQRELEEARASRDEIFAQSKESEKKLKSLEAEILQLQEELASSERARRHAEQERDELADEITNSASGKSALLDEKRRLEARIAQLEEELEEEQSNMELLNDRFRKTTLQVDTLNAELAAERSAAQKSDNARQQLERQNKELKAKLQELEGAVKSKFKATISALEAKIGQLEEQLEQEAKERAAANKLVRRTEKKLKEIFMQVEDERRHADQYKEQMEKANARMKQLKRQLEEAEEEATRANASRRKLQRELDDATEANEGLSREVSTLKNRLRRGGPISFSSSRSGRRQLHLEGASLELSDDDTESKTSDVNETQPPQSE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
92
Zebrafish
92
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35580 As Substrate

Site PTM Type Enzyme
Y13 Phosphorylation
R18 Methylation
Y22 Phosphorylation
T26 Phosphorylation
T31 Phosphorylation
K34 Ubiquitination
S40 Phosphorylation
K51 Ubiquitination
K68 Ubiquitination
K73 Ubiquitination
K78 Acetylation
K83 Ubiquitination
S85 Phosphorylation
K86 Ubiquitination
T94 Phosphorylation
Y155 Phosphorylation
R163 Methylation
S173 Phosphorylation
K184 Ubiquitination
K206 Ubiquitination
S227 Phosphorylation
K232 Ubiquitination
T233 Phosphorylation
K235 Ubiquitination
K244 Ubiquitination
K268 Ubiquitination
Y285 Phosphorylation
Y307 Phosphorylation
K362 Acetylation
Y407 Phosphorylation
K410 Acetylation
K410 Ubiquitination
K442 Acetylation
K552 Acetylation
S559 Phosphorylation
K562 Ubiquitination
K572 Acetylation
K572 Ubiquitination
K587 Ubiquitination
K594 Methylation
R613 Methylation
K620 Ubiquitination
S641 Phosphorylation
K644 Ubiquitination
K658 Methylation
T667 Phosphorylation
K689 Acetylation
K689 Ubiquitination
R718 Methylation
T732 Phosphorylation
K744 Ubiquitination
Y761 Phosphorylation
S766 Phosphorylation
K767 Acetylation
K767 Ubiquitination
K809 Ubiquitination
K817 Ubiquitination
C823 S-Nitrosylation
Y826 Phosphorylation
K828 Acetylation
K828 Ubiquitination
K840 Ubiquitination
K842 Ubiquitination
K857 Ubiquitination
K881 Ubiquitination
K968 Ubiquitination
S995 Phosphorylation
R1007 Methylation
S1013 Phosphorylation
K1021 Acetylation
K1031 Ubiquitination
S1037 Phosphorylation
K1059 Ubiquitination
S1129 Phosphorylation
S1145 Phosphorylation
T1170 Phosphorylation
T1187 Phosphorylation
R1198 Methylation
T1203 Phosphorylation
K1224 Acetylation
T1231 Phosphorylation
K1247 Acetylation
K1267 Ubiquitination
S1312 Phosphorylation
S1335 Phosphorylation
K1345 Acetylation
T1376 Phosphorylation
K1379 Acetylation
K1396 Acetylation
K1399 Ubiquitination
Y1415 Phosphorylation
S1444 Phosphorylation
K1448 Acetylation
K1451 Acetylation
K1452 Acetylation
K1452 Methylation
K1452 Ubiquitination
K1461 Acetylation
K1484 Acetylation
K1484 Ubiquitination
S1487 Phosphorylation
R1545 Methylation
T1558 Phosphorylation
K1562 Ubiquitination
T1582 Phosphorylation
R1609 Methylation
K1645 Acetylation
K1649 Ubiquitination
S1698 Phosphorylation
S1699 Phosphorylation
T1768 Phosphorylation
K1800 Acetylation
K1800 Ubiquitination
K1874 Acetylation
S1899 Phosphorylation
S1922 Phosphorylation
T1923 Phosphorylation
R1930 Methylation
S1935 Phosphorylation
S1937 Phosphorylation Q05513 (PRKCZ)
S1938 Phosphorylation
S1939 Phosphorylation
R1940 Methylation
S1952 Phosphorylation P68400 (CSNK2A1)
S1956 Phosphorylation P68400 (CSNK2A1)
T1960 Phosphorylation P68400 (CSNK2A1)
S1962 Phosphorylation
T1964 Phosphorylation
S1965 Phosphorylation P68400 (CSNK2A1)
T1970 Phosphorylation
S1975 Phosphorylation P68400 (CSNK2A1)

Research Backgrounds

Function:

Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation (in the central part but not the margins of spreading cells), and lamellipodial extension; this function is mechanically antagonized by MYH9.

PTMs:

Phosphorylated by ABL2.

Subcellular Location:

Cell projection>Lamellipodium.
Note: Colocalizes with MCC at the leading edge of migrating cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 1 is expressed in cerebellum and spinal chord. Isoform 2 is expressed in cerebrum and retina. Isoform 3 is expressed in the cerebrum and to a much lower extent in cerebellum.

Subunit Structure:

Myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Interacts with PLEKHG6. Interacts with ECPAS. Interacts with KIF26B (By similarity). Interacts with LARP6. Interacts with MCC. Interacts with C9orf135.

Family&Domains:

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

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