MTHFD1 Antibody - #DF13159

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Product: MTHFD1 Antibody
Catalog: DF13159
Description: Rabbit polyclonal antibody to MTHFD1
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 101 kDa; 102kD(Calculated).
Uniprot: P11586
RRID: AB_2846119

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(91%), Horse(82%), Sheep(91%), Rabbit(100%), Dog(100%), Chicken(82%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
MTHFD1 Antibody detects endogenous levels of total MTHFD1.
RRID:
AB_2846119
Cite Format: Affinity Biosciences Cat# DF13159, RRID:AB_2846119.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

5,10 methylenetetrahydrofolate dehydrogenase 5,10 methylenetetrahydrofolate cyclohydrolase,10 formyltetrahydrofolate synthetase; C 1 tetrahydrofolate synthase cytoplasmic; C1 tetrahydrofolate synthase cytoplasmic; C1 THF synthase; C1-THF synthase; C1TC; C1TC_HUMAN; Cytoplasmic C 1 tetrahydrofolate synthase; Formyltetrahydrofolate synthetase; Methenyltetrahydrofolate cyclohydrolase; Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) methenyltetrahydrofolate cyclohydrolase formyltetrahydrofolate synthetase; Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 methenyltetrahydrofolate cyclohydrolase formyltetrahydrofolate synthetase; Methylenetetrahydrofolate dehydrogenase; MTHFC; MTHFD; MTHFD1; NADP dependent cyclohydrolase formyltetrahydrofolate synthetase; OTTHUMP00000180657;

Immunogens

Immunogen:
Uniprot:

P11586(C1TC_HUMAN) >>Visit HPA database.

Gene(ID):
MTHFD1(4522)
Expression:
P11586 C1TC_HUMAN:

Ubiquitous.

Sequence:
MAPAEILNGKEISAQIRARLKNQVTQLKEQVPGFTPRLAILQVGNRDDSNLYINVKLKAAEEIGIKATHIKLPRTTTESEVMKYITSLNEDSTVHGFLVQLPLDSENSINTEEVINAIAPEKDVDGLTSINAGRLARGDLNDCFIPCTPKGCLELIKETGVPIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTAHLDEEVNKGDILVVATGQPEMVKGEWIKPGAIVIDCGINYVPDDKKPNGRKVVGDVAYDEAKERASFITPVPGGVGPMTVAMLMQSTVESAKRFLEKFKPGKWMIQYNNLNLKTPVPSDIDISRSCKPKPIGKLAREIGLLSEEVELYGETKAKVLLSALERLKHRPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLYQNVFACVRQPSQGPTFGIKGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARIFHELTQTDKALFNRLVPSVNGVRRFSDIQIRRLKRLGIEKTDPTTLTDEEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQAPTEKGHTRTAQFDISVASEIMAVLALTTSLEDMRERLGKMVVASSKKGEPVSAEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADRIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLCPHVVVLVATVRALKMHGGGPTVTAGLPLPKAYIQENLELVEKGFSNLKKQIENARMFGIPVVVAVNAFKTDTESELDLISRLSREHGAFDAVKCTHWAEGGKGALALAQAVQRAAQAPSSFQLLYDLKLPVEDKIRIIAQKIYGADDIELLPEAQHKAEVYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFILPIRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYDIDLDPETEQVNGLF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Rabbit
100
Bovine
91
Sheep
91
Horse
82
Xenopus
82
Chicken
82
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P11586 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K10 Acetylation
K10 Ubiquitination
K21 Ubiquitination
T25 Phosphorylation
K28 Ubiquitination
T35 Phosphorylation
R37 Methylation
Y52 Phosphorylation
K56 Acetylation
K58 Ubiquitination
K66 Ubiquitination
K71 Ubiquitination
T148 Phosphorylation
K150 Ubiquitination
K157 Ubiquitination
C195 S-Nitrosylation
K198 Ubiquitination
K223 Sumoylation
K223 Ubiquitination
K228 Ubiquitination
K251 Ubiquitination
Y258 Phosphorylation
K262 Acetylation
K262 Ubiquitination
S266 Phosphorylation
T269 Phosphorylation
T279 Phosphorylation
K313 Ubiquitination
T314 Phosphorylation
S318 Phosphorylation
R324 Methylation
C326 S-Nitrosylation
K333 Ubiquitination
K352 Ubiquitination
K354 Ubiquitination
S358 Phosphorylation
K364 Ubiquitination
K370 Ubiquitination
Y402 Phosphorylation
S413 Phosphorylation
T471 Phosphorylation
K473 Acetylation
K473 Ubiquitination
R488 Methylation
S490 Phosphorylation
R495 Methylation
K504 Ubiquitination
T505 Phosphorylation
T511 Phosphorylation
R539 Methylation
K543 Ubiquitination
T545 Phosphorylation
K553 Acetylation
K553 Ubiquitination
K588 Ubiquitination
S601 Phosphorylation
S608 Phosphorylation
T612 Phosphorylation
K684 Ubiquitination
K721 Acetylation
K721 Ubiquitination
K733 Ubiquitination
K739 Acetylation
K739 Ubiquitination
T761 Phosphorylation
T763 Phosphorylation
S765 Phosphorylation
S771 Phosphorylation
K784 Ubiquitination
K793 Acetylation
K793 Ubiquitination
K819 Acetylation
K819 Ubiquitination
K825 Ubiquitination
K832 Ubiquitination
Y834 Phosphorylation
K848 Ubiquitination
K854 Ubiquitination
K866 Ubiquitination
S870 Phosphorylation
S872 Phosphorylation
T882 Phosphorylation

Research Backgrounds

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Homodimer.

Family&Domains:

This trifunctional enzyme consists of two major domains: an N-terminal part containing the methylene-THF dehydrogenase and cyclohydrolase activities and a larger C-terminal part containing formyl-THF synthetase activity.

In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.

In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.

Research Fields

· Metabolism > Metabolism of cofactors and vitamins > One carbon pool by folate.

· Metabolism > Global and overview maps > Metabolic pathways.

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