MAT2A Antibody - #DF13139

(12)  
Product: MAT2A Antibody
Catalog: DF13139
Description: Rabbit polyclonal antibody to MAT2A
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Dog, Xenopus
Mol.Wt.: 45 kDa; 44kD(Calculated).
Uniprot: P31153
RRID: AB_2846099

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
MAT2A Antibody detects endogenous levels of total MAT2A.
RRID:
AB_2846099
Cite Format: Affinity Biosciences Cat# DF13139, RRID:AB_2846099.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AdoMet synthase 2; AdoMet synthetase 2; AdoMet synthetase; AMS 2; AMS2; MAT 2; MAT 2A; MAT II; MAT-II; Mat2a; MATA 2; MATA2; MATII; Methionine adenosyltransferase 2; Methionine adenosyltransferase; Methionine adenosyltransferase II alpha; Methionine adenosyltransferase II; METK2_HUMAN; S adenosylmethionine synthetase gamma form; S adenosylmethionine synthetase isoform type 2; S-adenosylmethionine synthase isoform type-2; SAMS 2; SAMS2;

Immunogens

Immunogen:
Uniprot:

P31153(METK2_HUMAN) >>Visit HPA database.

Gene(ID):
MAT2A(4144)
Expression:
P31153 METK2_HUMAN:

Detected in kidney.

Sequence:
MNGQLNGFHEAFIEEGTFLFTSESVGEGHPDKICDQISDAVLDAHLQQDPDAKVACETVAKTGMILLAGEITSRAAVDYQKVVREAVKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGVHLDRNEEDIGAGDQGLMFGYATDETEECMPLTIVLAHKLNAKLAELRRNGTLPWLRPDSKTQVTVQYMQDRGAVLPIRVHTIVISVQHDEEVCLDEMRDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKKPIYQRTAAYGHFGRDSFPWEVPKKLKY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
92
Chicken
67
Pig
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P31153 As Substrate

Site PTM Type Enzyme
K53 Ubiquitination
K61 Ubiquitination
K81 Acetylation
K81 Ubiquitination
K88 Acetylation
K88 Ubiquitination
K97 Ubiquitination
Y101 Phosphorylation
K102 Ubiquitination
C104 S-Nitrosylation
S114 Phosphorylation
K163 Ubiquitination
R192 Methylation
K228 Ubiquitination
K234 Acetylation
K234 Ubiquitination
Y235 Phosphorylation
Y242 Phosphorylation
T262 Phosphorylation
K285 Ubiquitination
K289 Ubiquitination
S293 Phosphorylation
K303 Ubiquitination
K307 Ubiquitination
S319 Phosphorylation
Y320 Phosphorylation
K340 Sumoylation
K350 Ubiquitination
K351 Ubiquitination
K367 Ubiquitination
K368 Sumoylation
K368 Ubiquitination
Y377 Phosphorylation
S384 Phosphorylation
K394 Sumoylation

Research Backgrounds

Function:

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.

Tissue Specificity:

Detected in kidney.

Subunit Structure:

Heterotrimer; composed of a catalytic MAT2A homodimer that binds one regulatory MAT2B chain. Heterohexamer; composed of a central, catalytic MAT2A homotetramer flanked on either side by a regulatory MAT2B chain.

Family&Domains:

Belongs to the AdoMet synthase family.

Research Fields

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.