Product: MARS Antibody
Catalog: DF13138
Description: Rabbit polyclonal antibody to MARS
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 101 kDa; 101kD(Calculated).
Uniprot: P56192
RRID: AB_2846098

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(90%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
MARS Antibody detects endogenous levels of total MARS.
RRID:
AB_2846098
Cite Format: Affinity Biosciences Cat# DF13138, RRID:AB_2846098.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

cytoplasmic; FLJ35667; Mars; Methionine tRNA ligase 1; Methionine tRNA ligase 1 cytoplasmic; Methionine tRNA ligase; Methionine tRNA synthetase; Methionine--tRNA ligase; Methionyl tRNA synthetase cytoplasmic; Methionyl-tRNA synthetase; MetRS; MRS; MTRNS; SYMC_HUMAN;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MRLFVSDGVPGCLPVLAAAGRARGRAEVLISTVGPEDCVVPFLTRPKVPVLQLDSGNYLFSTSAICRYFFLLSGWEQDDLTNQWLEWEATELQPALSAALYYLVVQGKKGEDVLGSVRRALTHIDHSLSRQNCPFLAGETESLADIVLWGALYPLLQDPAYLPEELSALHSWFQTLSTQEPCQRAAETVLKQQGVLALRPYLQKQPQPSPAEGRAVTNEPEEEELATLSEEEIAMAVTAWEKGLESLPPLRPQQNPVLPVAGERNVLITSALPYVNNVPHLGNIIGCVLSADVFARYSRLRQWNTLYLCGTDEYGTATETKALEEGLTPQEICDKYHIIHADIYRWFNISFDIFGRTTTPQQTKITQDIFQQLLKRGFVLQDTVEQLRCEHCARFLADRFVEGVCPFCGYEEARGDQCDKCGKLINAVELKKPQCKVCRSCPVVQSSQHLFLDLPKLEKRLEEWLGRTLPGSDWTPNAQFITRSWLRDGLKPRCITRDLKWGTPVPLEGFEDKVFYVWFDATIGYLSITANYTDQWERWWKNPEQVDLYQFMAKDNVPFHSLVFPCSALGAEDNYTLVSHLIATEYLNYEDGKFSKSRGVGVFGDMAQDTGIPADIWRFYLLYIRPEGQDSAFSWTDLLLKNNSELLNNLGNFINRAGMFVSKFFGGYVPEMVLTPDDQRLLAHVTLELQHYHQLLEKVRIRDALRSILTISRHGNQYIQVNEPWKRIKGSEADRQRAGTVTGLAVNIAALLSVMLQPYMPTVSATIQAQLQLPPPACSILLTNFLCTLPAGHQIGTVSPLFQKLENDQIESLRQRFGGGQAKTSPKPAVVETVTTAKPQQIQALMDEVTKQGNIVRELKAQKADKNEVAAEVAKLLDLKKQLAVAEGKPPEAPKGKKKK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
90
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P56192 As Substrate

Site PTM Type Enzyme
S6 Phosphorylation
K109 Ubiquitination
S116 Phosphorylation
Y153 Phosphorylation
Y161 Phosphorylation
K191 Ubiquitination
K204 Ubiquitination
S209 Phosphorylation
S229 Phosphorylation Q9P2K8 (EIF2AK4)
K335 Ubiquitination
Y336 Phosphorylation
Y344 Phosphorylation
K364 Ubiquitination
K375 Acetylation
K375 Ubiquitination
R376 Methylation
S472 Phosphorylation Q9P2K8 (EIF2AK4)
T482 Phosphorylation
K500 Ubiquitination
Y549 Phosphorylation
S644 Phosphorylation
S662 Phosphorylation Q9P2K8 (EIF2AK4)
K663 Ubiquitination
T675 Phosphorylation
K698 Ubiquitination
K726 Acetylation
K726 Ubiquitination
K729 Ubiquitination
K823 Ubiquitination
T824 Phosphorylation
S825 Phosphorylation
T833 Phosphorylation
K838 Ubiquitination
K875 Ubiquitination
K881 Ubiquitination

Research Backgrounds

Function:

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Plays a role in the synthesis of ribosomal RNA in the nucleolus.

Subcellular Location:

Cytoplasm>Cytosol. Nucleus>Nucleolus.
Note: Localizes to the nucleolus in proliferative cells but disappears in quiescent cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Monomer. Part of a multisubunit complex that groups tRNA ligases for Arg (RARS1), Asp (DARS1), Gln (QARS1), Ile (IARS1), Leu (LARS1), Lys (KARS1), Met (MARS1) the bifunctional ligase for Glu and Pro (EPRS1) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18 Ref.24,. Forms a linear complex that contains MARS1, EEF1E1, EPRS1 and AIMP2 that is at the core of the multisubunit complex.

Family&Domains:

Belongs to the class-I aminoacyl-tRNA synthetase family.

Research Fields

· Genetic Information Processing > Translation > Aminoacyl-tRNA biosynthesis.

· Metabolism > Metabolism of other amino acids > Selenocompound metabolism.

Restrictive clause

 

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