LSM4 Antibody | Affinity Biosciences

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Western blot analysis of extracts from MCF7 cells, using LSM4 Antibody.

Product:	LSM4 Antibody
Catalog:	DF13133
Description:	Rabbit polyclonal antibody to LSM4
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.:	15 kDa; 15kD(Calculated).
Uniprot:	Q9Y4Z0
RRID:	AB_2846093

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(86%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(80%), Xenopus(86%)

Clonality:

Polyclonal

Specificity:

LSM4 Antibody detects endogenous levels of total LSM4.

RRID:

AB_2846093
Cite Format: Affinity Biosciences Cat# DF13133, RRID:AB_2846093.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Glycine rich protein; GRP; LSM 4; LSM4 homolog; LSM4 homolog U6 small nuclear RNA associated (S. cerevisiae); LSM4 homolog U6 small nuclear RNA associated; U6 small nuclear RNA associated; U6 snRNA associated Sm like protein 4; U6 snRNA associated Sm like protein; U6 snRNA associated Sm like protein LSm 4; U6 snRNA associated Sm like protein LSm4; YER 112W; YER112W;

Immunogens

Immunogen:

Uniprot:

Q9Y4Z0(LSM4_HUMAN) >>Visit HPA database.

Gene(ID):

LSM4(25804)

Sequence:

Q9Y4Z0 LSM4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLPLSLLKTAQNHPMLVELKNGETYNGHLVSCDNWMNINLREVICTSRDGDKFWRMPECYIRGSTIKYLRIPDEIIDMVKEEVVAKGRGRGGLQQQKQQKGRGMGGAGRGVFGGRGRGGIPGTGRGQPEKKPGRQAGKQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y4Z0 As Substrate

Q9Y4Z0

Site	PTM Type	Source
K52	Ubiquitination	Uniprot
C59	S-Nitrosylation	Uniprot
R62	Methylation	Uniprot
K67	Ubiquitination	Uniprot
K80	Ubiquitination	Uniprot
R88	Methylation	Uniprot
R90	Methylation	Uniprot
R102	Methylation	Uniprot
R109	Methylation	Uniprot
R115	Methylation	Uniprot
R117	Methylation	Uniprot
R125	Methylation	Uniprot
K138	Acetylation	Uniprot

Research Backgrounds

Q9Y4Z0_LSM4_HUMAN

Function:

Plays role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA.

Subcellular Location:

Nucleus.

Subunit Structure:

Component of the precatalytic spliceosome (spliceosome B complex). Component of the U4/U6-U5 tri-snRNP complex, a building block of the precatalytic spliceosome (spliceosome B complex). The U4/U6-U5 tri-snRNP complex is composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39, plus LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8. LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8 form a heptameric, ring-shaped subcomplex (the LSM2-8 complex) that is part of the U4/U6-U5 tri-snRNP complex and the precatalytic spliceosome.

Family&Domains:

Belongs to the snRNP Sm proteins family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.

· Genetic Information Processing > Transcription > Spliceosome.

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LSM4 Antibody - #DF13133