LIFR Antibody | Affinity Biosciences

Product:	LIFR Antibody
Catalog:	DF13129
Description:	Rabbit polyclonal antibody to LIFR
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.:	190 kDa; 124kD(Calculated).
Uniprot:	P42702
RRID:	AB_2846089

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(89%), Bovine(89%), Horse(89%), Sheep(89%), Dog(89%)

Clonality:

Polyclonal

Specificity:

LIFR Antibody detects endogenous levels of total LIFR.

RRID:

AB_2846089
Cite Format: Affinity Biosciences Cat# DF13129, RRID:AB_2846089.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD118; CD118 antigen; FLJ98106; FLJ99923; Leukemia inhibitory factor receptor alpha; Leukemia inhibitory factor receptor; LIF R; LIF receptor; LIF-R; Lifr; LIFR_HUMAN; SJS2; STWS; SWS;

Immunogens

Immunogen:

Uniprot:

P42702(LIFR_HUMAN) >>Visit HPA database.

Gene(ID):

LIFR(3977)

Sequence:

P42702 LIFR_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MMDIYVCLKRPSWMVDNKRMRTASNFQWLLSTFILLYLMNQVNSQKKGAPHDLKCVTNNLQVWNCSWKAPSGTGRGTDYEVCIENRSRSCYQLEKTSIKIPALSHGDYEITINSLHDFGSSTSKFTLNEQNVSLIPDTPEILNLSADFSTSTLYLKWNDRGSVFPHRSNVIWEIKVLRKESMELVKLVTHNTTLNGKDTLHHWSWASDMPLECAIHFVEIRCYIDNLHFSGLEEWSDWSPVKNISWIPDSQTKVFPQDKVILVGSDITFCCVSQEKVLSALIGHTNCPLIHLDGENVAIKIRNISVSASSGTNVVFTTEDNIFGTVIFAGYPPDTPQQLNCETHDLKEIICSWNPGRVTALVGPRATSYTLVESFSGKYVRLKRAEAPTNESYQLLFQMLPNQEIYNFTLNAHNPLGRSQSTILVNITEKVYPHTPTSFKVKDINSTAVKLSWHLPGNFAKINFLCEIEIKKSNSVQEQRNVTIKGVENSSYLVALDKLNPYTLYTFRIRCSTETFWKWSKWSNKKQHLTTEASPSKGPDTWREWSSDGKNLIIYWKPLPINEANGKILSYNVSCSSDEETQSLSEIPDPQHKAEIRLDKNDYIISVVAKNSVGSSPPSKIASMEIPNDDLKIEQVVGMGKGILLTWHYDPNMTCDYVIKWCNSSRSEPCLMDWRKVPSNSTETVIESDEFRPGIRYNFFLYGCRNQGYQLLRSMIGYIEELAPIVAPNFTVEDTSADSILVKWEDIPVEELRGFLRGYLFYFGKGERDTSKMRVLESGRSDIKVKNITDISQKTLRIADLQGKTSYHLVLRAYTDGGVGPEKSMYVVTKENSVGLIIAILIPVAVAVIVGVVTSILCYRKREWIKETFYPDIPNPENCKALQFQKSVCEGSSALKTLEMNPCTPNNVEVLETRSAFPKIEDTEIISPVAERPEDRSDAEPENHVVVSYCPPIIEEEIPNPAADEAGGTAQVIYIDVQSMYQPQAKPEEEQENDPVGGAGYKPQMHLPINSTVEDIAAEEDLDKTAGYRPQANVNTWNLVSPDSPRSIDSNSEIVSFGSPCSINSRQFLIPPKDEDSPKSNGGGWSFTNFFQNKPND

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Sheep

Dog

Xenopus

Chicken

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P42702 As Substrate

P42702

Site	PTM Type	Enzyme	Source
N131	N-Glycosylation		Uniprot
K179	Ubiquitination		Uniprot
K186	Ubiquitination		Uniprot
K253	Ubiquitination		Uniprot
N303	N-Glycosylation		Uniprot
Y369	Phosphorylation		Uniprot
Y379	Phosphorylation		Uniprot
N407	N-Glycosylation		Uniprot
N426	N-Glycosylation		Uniprot
S490	Phosphorylation		Uniprot
S491	Phosphorylation		Uniprot
Y492	Phosphorylation		Uniprot
K526	Ubiquitination		Uniprot
K610	Acetylation		Uniprot
S665	Phosphorylation		Uniprot
Y759	Phosphorylation		Uniprot
Y762	Phosphorylation		Uniprot
K804	Ubiquitination		Uniprot
Y807	Phosphorylation		Uniprot
S833	Phosphorylation		Uniprot
K866	Ubiquitination		Uniprot
K880	Acetylation		Uniprot
K880	Ubiquitination		Uniprot
K886	Acetylation		Uniprot
K886	Ubiquitination		Uniprot
S887	Phosphorylation		Uniprot
K896	Acetylation		Uniprot
T904	Phosphorylation		Uniprot
S927	Phosphorylation		Uniprot
Y974	Phosphorylation		Uniprot
S1041	Phosphorylation		Uniprot
S1044	Phosphorylation	P28482 (MAPK1) , P27361 (MAPK3)	Uniprot
S1047	Phosphorylation		Uniprot
S1059	Phosphorylation		Uniprot
S1077	Phosphorylation		Uniprot

Research Backgrounds

P42702_LIFR_HUMAN

Function:

Signal-transducing molecule. May have a common pathway with IL6ST. The soluble form inhibits the biological activity of LIF by blocking its binding to receptors on target cells.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Secreted.

Subunit Structure:

Heterodimer composed of LIFR and IL6ST. The heterodimer formed by LIFR and IL6ST interacts with the complex formed by CNTF and CNTFR.

Family&Domains:

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.

The box 1 motif is required for JAK interaction and/or activation.

Belongs to the type I cytokine receptor family. Type 2 subfamily.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

LIFR Antibody - #DF13129