LASS2 Antibody - #DF13124
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13124, RRID:AB_2846084.
Fold/Unfold
Ceramide synthase 2; CerS 2; CerS2; CERS2_HUMAN; FLJ10243; L3; LAG1 homolog ceramide synthase 2; LAG1 longevity assurance 2; LAG1 longevity assurance homolog 2; LASS 2; Longevity assurance (LAG1 S. cerevisiae) homolog 2; MGC987; SP 260; SP260; TMSG 1; TMSG1; Tumor metastasis suppressor; Tumor metastasis suppressor gene 1 protein; Tumor metastasis-suppressor gene 1 protein;
Immunogens
- Q96G23 CERS2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLQTLYDYFWWERLWLPVNLTWADLEDRDGRVYAKASDLYITLPLALLFLIVRYFFELYVATPLAALLNIKEKTRLRAPPNATLEHFYLTSGKQPKQVEVELLSRQSGLSGRQVERWFRRRRNQDRPSLLKKFREASWRFTFYLIAFIAGMAVIVDKPWFYDMKKVWEGYPIQSTIPSQYWYYMIELSFYWSLLFSIASDVKRKDFKEQIIHHVATIILISFSWFANYIRAGTLIMALHDSSDYLLESAKMFNYAGWKNTCNNIFIVFAIVFIITRLVILPFWILHCTLVYPLELYPAFFGYYFFNSMMGVLQLLHIFWAYLILRMAHKFITGKLVEDERSDREETESSEGEEAAAGGGAKSRPLANGHPILNNNHRKND
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96G23 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| N19 | N-Glycosylation | Uniprot | |
| Y88 | Phosphorylation | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| K131 | Ubiquitination | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| K334 | Acetylation | Uniprot | |
| K334 | Ubiquitination | Uniprot | |
| S341 | Phosphorylation | Uniprot | |
| T346 | Phosphorylation | Uniprot | |
| S348 | Phosphorylation | Uniprot | |
| S349 | Phosphorylation | Uniprot |
Research Backgrounds
Ceramide synthase that catalyzes formation of ceramide from sphinganine and acyl-CoA substrates, with high selectivity toward very-long (C22:0-C24:0) chain as acyl donor.
Acetylated. Deacetylation by SIRT3 increases enzyme activity and promotes mitochondrial ceramide accumulation.
Phosphorylated at the C-terminus by CK2, leading to increase the ceramide synthase activity.
Endoplasmic reticulum membrane>Multi-pass membrane protein.
Expressed in kidney, liver, brain, heart, placenta and lung.
Interacts with ATP6V0C, ASGR1, ASGR2 and SLC22A1/OCT1. Interacts with ELOV1, HSD17B12 and TECR.
The last loop motif confers selectivity toward behenoyl-CoA (docosanoyl-CoA; C22:0-CoA) and lignoceroyl-CoA (tetracosanoyl-CoA; C24:0-CoA) as acyl donors.
Research Fields
· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)
· Metabolism > Lipid metabolism > Sphingolipid metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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