Kv1.3/KCNA3 Antibody | Affinity Biosciences

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Western blot analysis of extracts from A549 cells, using Kv1.

DF13118 at 1/100 staining Human mammary cancer by IHC-P.

DF13118 at 1/100 staining Human lung cancer by IHC-P.

Product:	Kv1.3/KCNA3 Antibody
Catalog:	DF13118
Description:	Rabbit polyclonal antibody to Kv1.3/KCNA3
Application:	WB IHC
Reactivity:	Human, Mouse
Mol.Wt.:	75 kDa; 64kD(Calculated).
Uniprot:	P22001
RRID:	AB_2846078

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

Kv1.3/KCNA3 Antibody detects endogenous levels of total Kv1.3/KCNA3.

RRID:

AB_2846078
Cite Format: Affinity Biosciences Cat# DF13118, RRID:AB_2846078.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

HGK 5; HGK5; HLK 3; HLK3; HPCN 3; HPCN3; HuKIII; KCNA 3; Kcna3; KCNA3_HUMAN; KV1.3; MK 3; MK3; OTTHUMP00000032397; PCN 3; PCN3; Potassium channel 3; Potassium voltage gated channel shaker related subfamily member 3; Potassium voltage gated channel subfamily A member 3; Potassium voltage-gated channel subfamily A member 3; Type n potassium channel; Voltage gated potassium channel protein Kv1.3; Voltage gated potassium channel subunit Kv1.3; Voltage-gated K(+) channel HuKIII; Voltage-gated potassium channel subunit Kv1.3;

Immunogens

Immunogen:

Uniprot:

P22001(KCNA3_HUMAN) >>Visit HPA database.

Gene(ID):

KCNA3(3738)

Sequence:

P22001 KCNA3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDERLSLLRSPPPPSARHRAHPPQRPASSGGAHTLVNHGYAEPAAGRELPPDMTVVPGDHLLEPEVADGGGAPPQGGCGGGGCDRYEPLPPSLPAAGEQDCCGERVVINISGLRFETQLKTLCQFPETLLGDPKRRMRYFDPLRNEYFFDRNRPSFDAILYYYQSGGRIRRPVNVPIDIFSEEIRFYQLGEEAMEKFREDEGFLREEERPLPRRDFQRQVWLLFEYPESSGPARGIAIVSVLVILISIVIFCLETLPEFRDEKDYPASTSQDSFEAAGNSTSGSRAGASSFSDPFFVVETLCIIWFSFELLVRFFACPSKATFSRNIMNLIDIVAIIPYFITLGTELAERQGNGQQAMSLAILRVIRLVRVFRIFKLSRHSKGLQILGQTLKASMRELGLLIFFLFIGVILFSSAVYFAEADDPTSGFSSIPDAFWWAVVTMTTVGYGDMHPVTIGGKIVGSLCAIAGVLTIALPVPVIVSNFNYFYHRETEGEEQSQYMHVGSCQHLSSSAEELRKARSNSTLSKSEYMVIEEGGMNHSAFPQTPFKTGNSTATCTTNNNPNSCVNIKKIFTDV

PTMs - P22001 As Substrate

P22001

Site	PTM Type	Enzyme	Source
S10	Phosphorylation		Uniprot
K120	Ubiquitination		Uniprot
K134	Ubiquitination		Uniprot
Y161	Phosphorylation	Q16620 (NTRK2)	Uniprot
Y162	Phosphorylation	Q16620 (NTRK2)	Uniprot
Y163	Phosphorylation	Q16620 (NTRK2)	Uniprot
Y187	Phosphorylation	P12931 (SRC)	Uniprot
K196	Ubiquitination		Uniprot
S378	Phosphorylation		Uniprot
Y447	Phosphorylation	P12931 (SRC)	Uniprot
Y499	Phosphorylation	P12931 (SRC) , Q16620 (NTRK2)	Uniprot
S504	Phosphorylation		Uniprot
S511	Phosphorylation		Uniprot
S520	Phosphorylation		Uniprot
T523	Phosphorylation		Uniprot
S525	Phosphorylation		Uniprot
K526	Ubiquitination		Uniprot
S527	Phosphorylation		Uniprot
Y529	Phosphorylation		Uniprot
S564	Phosphorylation		Uniprot
K569	Ubiquitination		Uniprot
K570	Ubiquitination		Uniprot

Research Backgrounds

P22001_KCNA3_HUMAN

Function:

Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

PTMs:

N-glycosylation promotes the cell surface expression.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Subunit Structure:

Heterotetramer of potassium channel proteins. Binds PDZ domains of DLG1, DLG2 and DLG4 (By similarity).

Family&Domains:

The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.3/KCNA3 sub-subfamily.

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Kv1.3/KCNA3 Antibody - #DF13118