KLF6 Antibody | Affinity Biosciences

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Cites

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Western blot analysis of extracts from PC-3 cells, using KLF6 Antibody.

Figure 2 The expression level of chemokines, adhesion molecules, and KLF6 expression in histamine-treated hNECs were repressed by febuxostat.

Product:	KLF6 Antibody
Catalog:	DF13114
Description:	Rabbit polyclonal antibody to KLF6
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.:	43 kDa; 32kD(Calculated).
Uniprot:	Q99612
RRID:	AB_2846074

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(80%), Bovine(80%), Sheep(80%), Rabbit(92%), Dog(80%)

Clonality:

Polyclonal

Specificity:

KLF6 Antibody detects endogenous levels of total KLF6.

RRID:

AB_2846074
Cite Format: Affinity Biosciences Cat# DF13114, RRID:AB_2846074.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:

Uniprot:

Q99612(KLF6_HUMAN) >>Visit HPA database.

Gene(ID):

KLF6(1316)

Expression:

Q99612 KLF6_HUMAN:

Highly expressed in placenta followed by spleen, thymus, prostate, testis, small intestine and colon. Weakly expressed in pancreas, lung, liver, heart and skeletal muscle. Also expressed in fetal brain, spleen and thymus.

Sequence:

Q99612 KLF6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDVLPMCSIFQELQIVHETGYFSALPSLEEYWQQTCLELERYLQSEPCYVSASEIKFDSQEDLWTKIILAREKKEESELKISSSPPEDTLISPSFCYNLETNSLNSDVSSESSDSSEELSPTAKFTSDPIGEVLVSSGKLSSSVTSTPPSSPELSREPSQLWGCVPGELPSPGKVRSGTSGKPGDKGNGDASPDGRRRVHRCHFNGCRKVYTKSSHLKAHQRTHTGEKPYRCSWEGCEWRFARSDELTRHFRKHTGAKPFKCSHCDRCFSRSDHLALHMKRHL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Pig

Bovine

Sheep

Dog

Xenopus

Chicken

Horse

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99612 As Substrate

Q99612

Site	PTM Type	Source
K66	Ubiquitination	Uniprot
T147	Phosphorylation	Uniprot
S150	Phosphorylation	Uniprot
S151	Phosphorylation	Uniprot
S171	Phosphorylation	Uniprot
S192	Phosphorylation	Uniprot
K209	Acetylation	Uniprot
K213	Acetylation	Uniprot
K218	Acetylation	Uniprot
T223	Phosphorylation	Uniprot
T225	Phosphorylation	Uniprot
K228	Acetylation	Uniprot
S233	Phosphorylation	Uniprot

Research Backgrounds

Q99612_KLF6_HUMAN

Function:

Transcriptional activator (By similarity). Binds a GC box motif. Could play a role in B-cell growth and development.

Subcellular Location:

Nucleus.

Tissue Specificity:

Family&Domains:

The acidic N-terminal part may favor interaction with the basic domain of transcription factors.

The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. In KLF6, the motif is inactive.

Belongs to the krueppel C2H2-type zinc-finger protein family.

References

1). miR‑181a‑5p regulates the proliferation and apoptosis of glomerular mesangial cells by targeting KLF6. Experimental and Therapeutic Medicine, 2020 (PubMed: 32742352) [IF=2.7]

Application: WB Species: human Sample: glomerular mesangial cell

Figure 7.| miR‑181a‑5p may regulate human glomerular mesangial cell proliferation and apoptosis via the Wnt/β‑catenin signaling pathway. (A) The protein expression levels of the associated factors in different groups were assessed by western blot analysis and (B) the results quantified. **P<0.01, *P<0.05. miR,microRNA; KLF6, kruppel-like factor 6; cells were treated with miR mimics control NC, negative control (cells were treated with miR mimics control).

2). FebuxostatAlleviatesAllergicRhinitis by Inhibiting Inflammation and Monocyte Adhesion in Human Nasal Epithelial Cells via Regulating KLF6. Evidence-based Complementary and Alternative Medicine, 2022 (PubMed: 36118091)

Application: WB Species: Human Sample: hNECs

Figure 2 The expression level of chemokines, adhesion molecules, and KLF6 expression in histamine-treated hNECs were repressed by febuxostat. (a). The expression level of chemokines was measured by the RT-qPCR assay, (b) the production of chemokines was evaluated by the ELISA assay, (c) the expression level of CAMs was checked by the RT-qPCR assay, (d) the expression level of CAMs was measured by the Western blotting assay (p < 0.05 and p < 0.01), (e) the adhesion between hNECs and U937 monocytes was inhibited by febuxostat. The attached U937 monocytes were detected using the calcein-AM staining assay (p < 0.05 and p < 0.01), (f) the expression level of KLF6 was evaluated by the RT-qPCR assay, and (g) the expression level of KLF6 was determined by the Western blotting assay (p < 0.05 and p < 0.01). Immunofluorescence magnification was 10×.

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KLF6 Antibody - #DF13114