KLF10 Antibody - #DF13112

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Product: KLF10 Antibody
Catalog: DF13112
Description: Rabbit polyclonal antibody to KLF10
Application: WB
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 51 kDa; 53kD(Calculated).
Uniprot: Q13118
RRID: AB_2846072

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(89%)
Clonality:
Polyclonal
Specificity:
KLF10 Antibody detects endogenous levels of total KLF10.
RRID:
AB_2846072
Cite Format: Affinity Biosciences Cat# DF13112, RRID:AB_2846072.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Early growth response, alpha; Early growth response, alpha like; EGR A; EGR alpha; EGR(A); EGR-alpha; EGRA; Egral; EGRalpha; Gdnfif; KLF 10; KLF10; KLF10_HUMAN; Krueppel like factor 10; Krueppel like factor10; Krueppel-like factor 10; Kruppel like factor 10; mGIF; TGF-beta- inducible early gene; TGFB inducible early growth response 1; TGFB inducible early growth response; TGFB inducible early growth response protein 1; TGFB-inducible early growth response protein 1; TIEG 1; TIEG; TIEG-1; TIEG1; Transforming growth factor beta inducible early growth response; Transforming growth factor beta inducible early growth response protein 1; Transforming growth factor-beta-inducible early growth response protein 1; Zinc finger transcription factor TIEG;

Immunogens

Immunogen:
Uniprot:

Q13118(KLF10_HUMAN) >>Visit HPA database.

Gene(ID):
KLF10(7071)
Sequence:
MLNFGASLQQTAEERMEMISERPKESMYSWNKTAEKSDFEAVEALMSMSCSWKSDFKKYVENRPVTPVSDLSEEENLLPGTPDFHTIPAFCLTPPYSPSDFEPSQVSNLMAPAPSTVHFKSLSDTAKPHIAAPFKEEEKSPVSAPKLPKAQATSVIRHTADAQLCNHQTCPMKAASILNYQNNSFRRRTHLNVEAARKNIPCAAVSPNRSKCERNTVADVDEKASAALYDFSVPSSETVICRSQPAPVSPQQKSVLVSPPAVSAGGVPPMPVICQMVPLPANNPVVTTVVPSTPPSQPPAVCPPVVFMGTQVPKGAVMFVVPQPVVQSSKPPVVSPNGTRLSPIAPAPGFSPSAAKVTPQIDSSRIRSHICSHPGCGKTYFKSSHLKAHTRTHTGEKPFSCSWKGCERRFARSDELSRHRRTHTGEKKFACPMCDRRFMRSDHLTKHARRHLSAKKLPNWQMEVSKLNDIALPPTPAPTQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
92
Xenopus
89
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13118 As Substrate

Site PTM Type Enzyme
K32 Ubiquitination
T66 Phosphorylation
T93 Phosphorylation P04049 (RAF1)
S97 Phosphorylation
K135 Sumoylation
K139 Ubiquitination
S140 Phosphorylation
K149 Sumoylation
K149 Ubiquitination
S184 Phosphorylation
S206 Phosphorylation P24941 (CDK2)
K223 Ubiquitination
S249 Phosphorylation
S351 Phosphorylation
K356 Ubiquitination
K378 Ubiquitination
S383 Phosphorylation
S384 Phosphorylation
K387 Ubiquitination
T392 Phosphorylation
T394 Phosphorylation
T422 Phosphorylation
T424 Phosphorylation
T445 Phosphorylation
K446 Ubiquitination
K456 Sumoylation

Research Backgrounds

Function:

Transcriptional repressor which binds to the consensus sequence 5'-GGTGTG-3'. Plays a role in the regulation of the circadian clock; binds to the GC box sequence in the promoter of the core clock component ARTNL/BMAL1 and represses its transcriptional activity. Regulates the circadian expression of genes involved in lipogenesis, gluconeogenesis, and glycolysis in the liver. Represses the expression of PCK2, a rate-limiting step enzyme of gluconeogenesis (By similarity). May play a role in the cell cycle regulation.

PTMs:

Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the Sp1 C2H2-type zinc-finger protein family.

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