IRF2BP2 Antibody - #DF13096

(3)  
Product: IRF2BP2 Antibody
Catalog: DF13096
Description: Rabbit polyclonal antibody to IRF2BP2
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Sheep, Dog, Chicken
Mol.Wt.: 61 kDa; 61kD(Calculated).
Uniprot: Q7Z5L9
RRID: AB_2846056

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Sheep(100%), Dog(100%), Chicken(86%)
Clonality:
Polyclonal
Specificity:
IRF2BP2 Antibody detects endogenous levels of total IRF2BP2.
RRID:
AB_2846056
Cite Format: Affinity Biosciences Cat# DF13096, RRID:AB_2846056.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

I2BP2_HUMAN; Interferon regulatory factor 2 binding protein 2; Interferon regulatory factor 2-binding protein 2; IRF-2-binding protein 2; IRF-2BP2; IRF2 binding protein 2; IRF2BP2;

Immunogens

Immunogen:
Uniprot:

Q7Z5L9(I2BP2_HUMAN) >>Visit HPA database.

Gene(ID):
IRF2BP2(359948)
Sequence:
MAAAVAVAAASRRQSCYLCDLPRMPWAMIWDFTEPVCRGCVNYEGADRVEFVIETARQLKRAHGCFPEGRSPPGAAASAAAKPPPLSAKDILLQQQQQLGHGGPEAAPRAPQALERYPLAAAAERPPRLGSDFGSSRPAASLAQPPTPQPPPVNGILVPNGFSKLEEPPELNRQSPNPRRGHAVPPTLVPLMNGSATPLPTALGLGGRAAASLAAVSGTAAASLGSAQPTDLGAHKRPASVSSSAAVEHEQREAAAKEKQPPPPAHRGPADSLSTAAGAAELSAEGAGKSRGSGEQDWVNRPKTVRDTLLALHQHGHSGPFESKFKKEPALTAGRLLGFEANGANGSKAVARTARKRKPSPEPEGEVGPPKINGEAQPWLSTSTEGLKIPMTPTSSFVSPPPPTASPHSNRTTPPEAAQNGQSPMAALILVADNAGGSHASKDANQVHSTTRRNSNSPPSPSSMNQRRLGPREVGGQGAGNTGGLEPVHPASLPDSSLATSAPLCCTLCHERLEDTHFVQCPSVPSHKFCFPCSRQSIKQQGASGEVYCPSGEKCPLVGSNVPWAFMQGEIATILAGDVKVKKERDS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Sheep
100
Dog
100
Chicken
86
Pig
56
Bovine
56
Horse
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7Z5L9 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S15 Phosphorylation
Y17 Phosphorylation
S71 Phosphorylation
S78 Phosphorylation
S87 Phosphorylation
R109 Methylation
R116 Methylation
R125 Methylation
S131 Phosphorylation
S141 Phosphorylation
T147 Phosphorylation
S175 Phosphorylation
T197 Phosphorylation
S240 Phosphorylation
S242 Phosphorylation
S243 Phosphorylation
S244 Phosphorylation
S283 Phosphorylation
K289 Ubiquitination
S290 Phosphorylation
R291 Methylation
S293 Phosphorylation
K303 Ubiquitination
S318 Phosphorylation
S323 Phosphorylation
K324 Ubiquitination
K327 Ubiquitination
S360 Phosphorylation
S381 Phosphorylation
S383 Phosphorylation
T392 Phosphorylation
S399 Phosphorylation
T404 Phosphorylation
S406 Phosphorylation
S409 Phosphorylation
T412 Phosphorylation
T413 Phosphorylation
S423 Phosphorylation
S455 Phosphorylation
S457 Phosphorylation
S460 Phosphorylation
S462 Phosphorylation
S463 Phosphorylation
S492 Phosphorylation
K539 Ubiquitination

Research Backgrounds

Function:

Acts as a transcriptional corepressor in a IRF2-dependent manner; this repression is not mediated by histone deacetylase activities. Represses the NFAT1-dependent transactivation of NFAT-responsive promoters. Acts as a coactivator of VEGFA expression in cardiac and skeletal muscles. Plays a role in immature B-cell differentiation.

PTMs:

Phosphorylation at Ser-360 is required for nuclear targeting.

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with IRF2. Part of a corepressor complex containing IRF2 and IRF2BP1. Interacts with VGLL4.

Family&Domains:

The C-terminal RING-type zinc finger domain is sufficient for interaction with IRF2.

Belongs to the IRF2BP family.

Restrictive clause

 

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