HTATSF1 Antibody - #DF13078
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13078, RRID:AB_2846038.
Fold/Unfold
Cofactor required for Tat activation of HIV 1 transcription; dJ196E23.2; HIV Tat specific factor 1; HTATSF1; HTSF-1; HTSF1; TAT SF1; Tat-SF1;
Immunogens
- O43719 HTSF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGTNLDGNDEFDEQLRMQELYGDGKDGDTQTDAGGEPDSLGQQPTDTPYEWDLDKKAWFPKITEDFIATYQANYGFSNDGASSSTANVEDVHARTAEEPPQEKAPEPTDARKKGEKRKAESGWFHVEEDRNTNVYVSGLPPDITVDEFIQLMSKFGIIMRDPQTEEFKVKLYKDNQGNLKGDGLCCYLKRESVELALKLLDEDEIRGYKLHVEVAKFQLKGEYDASKKKKKCKDYKKKLSMQQKQLDWRPERRAGPSRMRHERVVIIKNMFHPMDFEDDPLVLNEIREDLRVECSKFGQIRKLLLFDRHPDGVASVSFRDPEEADYCIQTLDGRWFGGRQITAQAWDGTTDYQVEETSREREERLRGWEAFLNAPEANRGLRRSDSVSASERAGPSRARHFSEHPSTSKMNAQETATGMAFEEPIDEKKFEKTEDGGEFEEGASENNAKESSPEKEAEEGCPEKESEEGCPKRGFEGSCSQKESEEGNPVRGSEEDSPKKESKKKTLKNDCEENGLAKESEDDLNKESEEEVGPTKESEEDDSEKESDEDCSEKQSEDGSEREFEENGLEKDLDEEGSEKELHENVLDKELEENDSENSEFEDDGSEKVLDEEGSEREFDEDSDEKEEEEDTYEKVFDDESDEKEDEEYADEKGLEAADKKAEEGDADEKLFEESDDKEDEDADGKEVEDADEKLFEDDDSNEKLFDEEEDSSEKLFDDSDERGTLGGFGSVEEGPLSTGSSFILSSDDDDDDI
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O43719 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| T4 | Phosphorylation | Uniprot | |
| R17 | Methylation | Uniprot | |
| Y22 | Phosphorylation | Uniprot | |
| T30 | Phosphorylation | Uniprot | |
| T32 | Phosphorylation | Uniprot | |
| S40 | Phosphorylation | Uniprot | |
| T48 | Phosphorylation | Uniprot | |
| Y50 | Phosphorylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| Y71 | Phosphorylation | Uniprot | |
| Y75 | Phosphorylation | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| K104 | Ubiquitination | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| S122 | Phosphorylation | Uniprot | |
| S154 | Phosphorylation | Uniprot | |
| K169 | Acetylation | Uniprot | |
| K169 | Ubiquitination | Uniprot | |
| K174 | Acetylation | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| K181 | Ubiquitination | Uniprot | |
| K190 | Ubiquitination | Uniprot | |
| K199 | Ubiquitination | Uniprot | |
| R207 | Methylation | Uniprot | |
| K210 | Ubiquitination | Uniprot | |
| K217 | Ubiquitination | Uniprot | |
| K221 | Sumoylation | Uniprot | |
| K221 | Ubiquitination | Uniprot | |
| Y224 | Phosphorylation | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| K297 | Ubiquitination | Uniprot | |
| K303 | Ubiquitination | Uniprot | |
| R309 | Methylation | Uniprot | |
| Y327 | Phosphorylation | Uniprot | |
| Y353 | Phosphorylation | Uniprot | |
| R367 | Methylation | Uniprot | |
| R380 | Methylation | Uniprot | |
| S385 | Phosphorylation | Uniprot | |
| S387 | Phosphorylation | Uniprot | |
| S391 | Phosphorylation | Uniprot | |
| R393 | Methylation | Uniprot | |
| R400 | Methylation | Uniprot | |
| S403 | Phosphorylation | Uniprot | |
| S407 | Phosphorylation | Uniprot | |
| T408 | Phosphorylation | Uniprot | |
| S409 | Phosphorylation | Uniprot | |
| T416 | Phosphorylation | Uniprot | |
| K429 | Sumoylation | Uniprot | |
| T434 | Phosphorylation | Uniprot | |
| S445 | Phosphorylation | Uniprot | |
| S452 | Phosphorylation | Uniprot | |
| S453 | Phosphorylation | Uniprot | |
| S467 | Phosphorylation | Uniprot | |
| K473 | Ubiquitination | Uniprot | |
| S479 | Phosphorylation | Uniprot | |
| S481 | Phosphorylation | Uniprot | |
| S485 | Phosphorylation | Uniprot | |
| S494 | Phosphorylation | Uniprot | |
| S498 | Phosphorylation | Uniprot | |
| K504 | Acetylation | Uniprot | |
| K505 | Acetylation | Uniprot | |
| K506 | Acetylation | Uniprot | |
| S521 | Phosphorylation | Uniprot | |
| S529 | Phosphorylation | Uniprot | |
| S539 | Phosphorylation | Uniprot | |
| S544 | Phosphorylation | Uniprot | |
| S557 | Phosphorylation | Uniprot | |
| S561 | Phosphorylation | Uniprot | |
| S579 | Phosphorylation | Uniprot | |
| S597 | Phosphorylation | Uniprot | |
| S600 | Phosphorylation | Uniprot | |
| S607 | Phosphorylation | Uniprot | |
| S616 | Phosphorylation | Uniprot | |
| S624 | Phosphorylation | Uniprot | |
| T633 | Phosphorylation | Uniprot | |
| Y634 | Phosphorylation | Uniprot | |
| S642 | Phosphorylation | Uniprot | |
| Y650 | Phosphorylation | Uniprot | |
| S676 | Phosphorylation | Uniprot | |
| S702 | Phosphorylation | Uniprot | |
| K705 | Ubiquitination | Uniprot | |
| S713 | Phosphorylation | Uniprot | |
| S714 | Phosphorylation | Uniprot | |
| K716 | Ubiquitination | Uniprot | |
| S721 | Phosphorylation | Uniprot | |
| S739 | Phosphorylation | Uniprot | |
| T740 | Phosphorylation | Uniprot | |
| S743 | Phosphorylation | Uniprot | |
| S747 | Phosphorylation | Uniprot | |
| S748 | Phosphorylation | Uniprot |
Research Backgrounds
Functions as a general transcription factor playing a role in the process of transcriptional elongation. May mediate the reciprocal stimulatory effect of splicing on transcriptional elongation. In case of infection by HIV-1, it is up-regulated by the HIV-1 proteins NEF and gp120, acts as a cofactor required for the Tat-enhanced transcription of the virus.
Nucleus.
Widely expressed.
Component of a complex which is at least composed of HTATSF1/Tat-SF1, the P-TEFb complex components CDK9 and CCNT1, RNA polymerase II, SUPT5H, and NCL/nucleolin. Interacts with GTF2F2/RAP30 and POLR2A. Interacts with TCERG1/CA150. Interacts with SF3A2/SAP62 and the spliceosomal U small nuclear ribonucleoproteins (snRNPs).
The RRM domains mediate interaction with U snRNPs.
Belongs to the HTATSF1 family.
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