HMGCL Antibody - #DF13063
| Product: | HMGCL Antibody |
| Catalog: | DF13063 |
| Description: | Rabbit polyclonal antibody to HMGCL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 34 kDa; 34kD(Calculated). |
| Uniprot: | P35914 |
| RRID: | AB_2846024 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13063, RRID:AB_2846024.
Fold/Unfold
3 hydroxy 3 methylglutaryl CoA lyase; 3 hydroxy 3 methylglutaryl Coenzyme A lyase; 3 hydroxymethyl 3 methylglutaryl Coenzyme A lyase (hydroxymethylglutaricaciduria); 3 hydroxymethyl 3 methylglutaryl Coenzyme A lyase; 3-hydroxy-3-methylglutarate-CoA lyase; HL; HMG CoA lyase; HMG CoA Lyase Deficiency; HMG-CoA lyase; HMGCL; HMGCL_HUMAN; Hydroxymethylglutaricaciduria; Hydroxymethylglutaryl CoA lyase; Hydroxymethylglutaryl CoA lyase mitochondrial; Hydroxymethylglutaryl-CoA lyase; Mitochondrial 3 hydroxy 3 methylglutaryl CoA lyase; mitochondrial; MS725; OTTHUMP00000044830;
Immunogens
Highest expression in liver. Expressed in pancreas, kidney, intestine, testis, fibroblasts and lymphoblasts. Very low expression in brain and skeletal muscle. The relative expression of isoform 2 (at mRNA level) is highest in heart (30%), skeletal muscle (22%), and brain (14%).
- P35914 HMGCL_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAMRKALPRRLVGLASLRAVSTSSMGTLPKRVKIVEVGPRDGLQNEKNIVSTPVKIKLIDMLSEAGLSVIETTSFVSPKWVPQMGDHTEVLKGIQKFPGINYPVLTPNLKGFEAAVAAGAKEVVIFGAASELFTKKNINCSIEESFQRFDAILKAAQSANISVRGYVSCALGCPYEGKISPAKVAEVTKKFYSMGCYEISLGDTIGVGTPGIMKDMLSAVMQEVPLAALAVHCHDTYGQALANTLMALQMGVSVVDSSVAGLGGCPYAQGASGNLATEDLVYMLEGLGIHTGVNLQKLLEAGNFICQALNRKTSSKVAQATCKL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P35914 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S17 | Phosphorylation | Uniprot | |
| S22 | Phosphorylation | Uniprot | |
| T23 | Phosphorylation | Uniprot | |
| K48 | Acetylation | Uniprot | |
| K48 | Ubiquitination | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| K80 | Methylation | Uniprot | |
| K93 | Acetylation | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| K137 | Acetylation | Uniprot | |
| Y198 | Phosphorylation | Uniprot | |
| C323 | S-Nitrosylation | Uniprot | |
| K324 | Acetylation | Uniprot |
Research Backgrounds
Mitochondrial 3-hydroxymethyl-3-methylglutaryl-CoA lyase that catalyzes a cation-dependent cleavage of (S)-3-hydroxy-3-methylglutaryl-CoA into acetyl-CoA and acetoacetate, a key step in ketogenesis. Terminal step in leucine catabolism. Ketone bodies (beta-hydroxybutyrate, acetoacetate and acetone) are essential as an alternative source of energy to glucose, as lipid precursors and as regulators of metabolism.
Mitochondrion matrix. Peroxisome.
Note: Unprocessed form is peroxisomal.
Highest expression in liver. Expressed in pancreas, kidney, intestine, testis, fibroblasts and lymphoblasts. Very low expression in brain and skeletal muscle. The relative expression of isoform 2 (at mRNA level) is highest in heart (30%), skeletal muscle (22%), and brain (14%).
Homodimer; disulfide-linked. Can also form homotetramers.
Belongs to the HMG-CoA lyase family.
Research Fields
· Cellular Processes > Transport and catabolism > Peroxisome. (View pathway)
· Metabolism > Lipid metabolism > Synthesis and degradation of ketone bodies.
· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine degradation.
· Metabolism > Carbohydrate metabolism > Butanoate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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