HAS3 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Mouse liver tissue , using HAS3 Antibody.

Product:	HAS3 Antibody
Catalog:	DF13055
Description:	Rabbit polyclonal antibody to HAS3
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	63 kDa; 63kD(Calculated).
Uniprot:	O00219
RRID:	AB_2846016

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(91%), Xenopus(91%)

Clonality:

Polyclonal

Specificity:

HAS3 Antibody detects endogenous levels of total HAS3.

RRID:

AB_2846016
Cite Format: Affinity Biosciences Cat# DF13055, RRID:AB_2846016.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

HA synthase 3; Has3; HAS3_HUMAN; Hyaluronan synthase 3; Hyaluronate synthase 3; Hyaluronic acid synthase 3;

Immunogens

Immunogen:

Uniprot:

O00219(HYAS3_HUMAN) >>Visit HPA database.

Gene(ID):

HAS3(3038)

Sequence:

O00219 HYAS3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPVQLTTALRVVGTSLFALAVLGGILAAYVTGYQFIHTEKHYLSFGLYGAILGLHLLIQSLFAFLEHRRMRRAGQALKLPSPRRGSVALCIAAYQEDPDYLRKCLRSAQRISFPDLKVVMVVDGNRQEDAYMLDIFHEVLGGTEQAGFFVWRSNFHEAGEGETEASLQEGMDRVRDVVRASTFSCIMQKWGGKREVMYTAFKALGDSVDYIQVCDSDTVLDPACTIEMLRVLEEDPQVGGVGGDVQILNKYDSWISFLSSVRYWMAFNVERACQSYFGCVQCISGPLGMYRNSLLQQFLEDWYHQKFLGSKCSFGDDRHLTNRVLSLGYRTKYTARSKCLTETPTKYLRWLNQQTRWSKSYFREWLYNSLWFHKHHLWMTYESVVTGFFPFFLIATVIQLFYRGRIWNILLFLLTVQLVGIIKATYACFLRGNAEMIFMSLYSLLYMSSLLPAKIFAIATINKSGWGTSGRKTIVVNFIGLIPVSIWVAVLLGGLAYTAYCQDLFSETELAFLVSGAILYGCYWVALLMLYLAIIARRCGKKPEQYSLAFAEV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Dog

100

Zebrafish

100

Rabbit

100

Xenopus

Chicken

Sheep

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00219 As Substrate

O00219

Site	PTM Type	Source
T6	Phosphorylation	Uniprot
K78	Ubiquitination	Uniprot
Y333	Phosphorylation	Uniprot
Y347	Phosphorylation	Uniprot
K542	Ubiquitination	Uniprot

Research Backgrounds

O00219_HYAS3_HUMAN

Function:

Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction (By similarity).

Subcellular Location:

Membrane>Multi-pass membrane protein.

Family&Domains:

Belongs to the NodC/HAS family.

References

1). Interfering with hyaluronic acid metabolism suppresses glioma cell proliferation by regulating autophagy. Cell Death & Disease, 2021 (PubMed: 33986244) [IF=9.0]

Application: WB Species: human Sample: human glioma tissues and normal brain tissues

Fig. 1 | Hyaluronic acid, HAS3, and CD44 are increased in glioma tissues and negatively correlated with the prognosis of glioma. A Relative concentration of HA in human glioma tissues of different grades and normal brain tissues by ELISA. B Representative images of IHC staining for HA,HAS3, and CD44 in human glioma tissues of different grades and normal brain tissues. Scale bar: 50 μm.

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HAS3 Antibody - #DF13055