Product: GBF1 Antibody
Catalog: DF13034
Description: Rabbit polyclonal antibody to GBF1
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 206 kDa; 206kD(Calculated).
Uniprot: Q92538
RRID: AB_2845995

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Horse(92%), Sheep(100%), Rabbit(92%), Dog(92%)
Clonality:
Polyclonal
Specificity:
GBF1 Antibody detects endogenous levels of total GBF1.
RRID:
AB_2845995
Cite Format: Affinity Biosciences Cat# DF13034, RRID:AB_2845995.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ARF1GEF; BFA resistant GEF 1; BFA-resistant GEF 1; FLJ21263; FLJ21500; GBF1; GBF1_HUMAN; golgi brefeldin A resistant guanine nucleotide exchange factor 1; Golgi specific brefeldin A resistance factor 1; Golgi specific Brefeldin A-resistance factor; Golgi-specific brefeldin A-resistance guanine nucleotide exchange; Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1; KIAA0248; MGC134877; MGC134878;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q92538 GBF1_HUMAN:

Ubiquitous.

Sequence:
MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSCEIVDGTREASNTERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Sheep
100
Horse
92
Dog
92
Rabbit
92
Xenopus
58
Chicken
58
Zebrafish
50
Bovine
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92538 As Substrate

Site PTM Type Enzyme
S174 Phosphorylation
K212 Ubiquitination
S224 Phosphorylation
S233 Phosphorylation
S292 Phosphorylation
S297 Phosphorylation
T307 Phosphorylation
S314 Phosphorylation
S349 Phosphorylation
S352 Phosphorylation
T361 Phosphorylation
S362 Phosphorylation
S369 Phosphorylation
S371 Phosphorylation
Y377 Phosphorylation
Y473 Phosphorylation
S476 Phosphorylation
S486 Phosphorylation
T507 Phosphorylation
Y515 Phosphorylation
S599 Phosphorylation
T601 Phosphorylation
K605 Ubiquitination
K606 Ubiquitination
S612 Phosphorylation
S659 Phosphorylation
S662 Phosphorylation
K697 Ubiquitination
K713 Ubiquitination
K716 Ubiquitination
S756 Phosphorylation
K844 Ubiquitination
K859 Acetylation
K859 Ubiquitination
K865 Ubiquitination
Y898 Phosphorylation
K953 Ubiquitination
S991 Phosphorylation
K1004 Ubiquitination
K1112 Ubiquitination
K1124 Ubiquitination
S1298 Phosphorylation
S1300 Phosphorylation
S1304 Phosphorylation
S1311 Phosphorylation
Y1316 Phosphorylation
T1317 Phosphorylation
S1318 Phosphorylation
S1320 Phosphorylation
Y1323 Phosphorylation
T1324 Phosphorylation
S1335 Phosphorylation
T1337 Phosphorylation Q13131 (PRKAA1)
S1344 Phosphorylation
K1351 Ubiquitination
S1367 Phosphorylation
S1374 Phosphorylation
K1416 Ubiquitination
S1453 Phosphorylation
K1454 Acetylation
S1457 Phosphorylation
T1464 Phosphorylation
S1475 Phosphorylation
K1529 Ubiquitination
Y1567 Phosphorylation
K1579 Ubiquitination
K1591 Ubiquitination
T1680 Phosphorylation
S1686 Phosphorylation
K1718 Ubiquitination
T1750 Phosphorylation
S1773 Phosphorylation
S1778 Phosphorylation
S1779 Phosphorylation
S1780 Phosphorylation
S1781 Phosphorylation
S1784 Phosphorylation
S1788 Phosphorylation
S1789 Phosphorylation
S1791 Phosphorylation

Research Backgrounds

Function:

Guanine-nucleotide exchange factor (GEF) for members of the Arf family of small GTPases involved in trafficking in the early secretory pathway; its GEF activity initiates the coating of nascent vesicles via the localized generation of activated ARFs through replacement of GDP with GTP. Recruitment to cis-Golgi membranes requires membrane association of Arf-GDP and can be regulated by ARF1, ARF3, ARF4 and ARF5. Involved in the recruitment of the COPI coat complex to the endoplasmic reticulum exit sites (ERES), and the endoplasmic reticulum-Golgi intermediate (ERGIC) and cis-Golgi compartments which implicates ARF1 activation. Involved in COPI vesicle-dependent retrograde transport from the ERGIC and cis-Golgi compartments to the endoplasmic reticulum (ER). Involved in the trans-Golgi network recruitment of GGA1, GGA2, GGA3, BIG1, BIG2, and the AP-1 adapter protein complex related to chlathrin-dependent transport; the function requires its GEF activity (probably at least in part on ARF4 and ARF5). Has GEF activity towards ARF1. Has in vitro GEF activity towards ARF5 (By similarity). Involved in the processing of PSAP. Required for the assembly of the Golgi apparatus. The AMPK-phosphorylated form is involved in Golgi disassembly during mitotis and under stress conditions. May be involved in the COPI vesicle-dependent recruitment of PNPLA2 to lipid droplets; however, this function is under debate. In neutrophils, involved in G protein-coupled receptor (GPCR)-mediated chemotaxis und superoxide production. Proposed to be recruited by phosphatidylinositol-phosphates generated upon GPCR stimulation to the leading edge where it recruits and activates ARF1, and is involved in recruitment of GIT2 and the NADPH oxidase complex.

PTMs:

AMPK-mediated phosphorylation at Thr-1337 is induced by 2-deoxyglucose (2-DG) and AICA ribonucleotide, and occurs during mitosis leading to membrane disassociation and inactivation of ARF1 during mitosis.

Subcellular Location:

Golgi apparatus>cis-Golgi network. Endoplasmic reticulum-Golgi intermediate compartment. Golgi apparatus>trans-Golgi network. Golgi apparatus. Cytoplasm. Lipid droplet. Membrane>Peripheral membrane protein.
Note: Cycles rapidly on and off early Golgi membranes (PubMed:15616190). Stabilized on membranes when complexed with ARF1-GDP and is released from both ARF1 and membranes after it catalyzes GDP displacement and ARF1 binds GTP. Continuous cycles of recruitment and dissociation of GBF1 to membranes are required for sustained ARF activation and COP I recruitment (PubMed:15813748). In neutrophils is translocated from the Golgi to the leading edge upon GPCR stimulation (PubMed:22573891). Localization to lipid droplets is questionable (PubMed:22185782).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Can form homodimers and probably homotetramers. Interacts with COPG1; the interaction is independent on ARF1 activation. Interacts with ARF1, ARF3, ARF4 and ARF5. Interacts with RAB1B (GTP-bound form); required for GBF1 membrane association. Interacts with GGA1, GGA2 and GGA3. Interacts with USO1. Interacts (via SEC7 domain) with PNPLA2 (via C-terminus); the interaction is direct. Interacts with ARMH3.

(Microbial infection) Interacts with poliovirus protein 3A.

Family&Domains:

The DCB (dimerization and cyclophiln-binding) and HUS (homology upstream of Sec7) domains are necessary for dimerization. The DCB domain is proposed to support constitutive homodimerization; the HUS domain interacts with the DCB domain which may occur intramolecular or intermolecuar (By similarity).

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

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