GAMT Antibody - #DF13032

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Product: GAMT Antibody
Catalog: DF13032
Description: Rabbit polyclonal antibody to GAMT
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Zebrafish
Prediction: Pig, Bovine, Horse, Dog, Chicken
Mol.Wt.: 26 kDa; 26kD(Calculated).
Uniprot: Q14353
RRID: AB_2845993

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Zebrafish
Prediction:
Pig(100%), Bovine(90%), Horse(80%), Dog(100%), Chicken(80%)
Clonality:
Polyclonal
Specificity:
GAMT Antibody detects endogenous levels of total GAMT.
RRID:
AB_2845993
Cite Format: Affinity Biosciences Cat# DF13032, RRID:AB_2845993.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CCDS2; Epididymis secretory protein Li 20; GAMT; GAMT_HUMAN; Guanidinoacetate N methyltransferase; Guanidinoacetate N-methyltransferase; HEL-S-20; PIG2; TP53I2;

Immunogens

Immunogen:
Uniprot:

Q14353(GAMT_HUMAN) >>Visit HPA database.

Gene(ID):
GAMT(2593)
Expression:
Q14353 GAMT_HUMAN:

Expressed in liver.

Sequence:
MSAPSATPIFAPGENCSPAWGAAPAAYDAADTHLRILGKPVMERWETPYMHALAAAASSKGGRVLEVGFGMAIAASKVQEAPIDEHWIIECNDGVFQRLRDWAPRQTHKVIPLKGLWEDVAPTLPDGHFDGILYDTYPLSEETWHTHQFNFIKNHAFRLLKPGGVLTYCNLTSWGELMKSKYSDITIMFEETQVPALLEAGFRRENIRTEVMALVPPADCRYYAFPQMITPLVTKG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Bovine
90
Horse
80
Chicken
80
Xenopus
70
Zebrafish
60
Sheep
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14353 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S17 Phosphorylation
Y27 Phosphorylation
K39 Ubiquitination
K60 Ubiquitination
K109 Ubiquitination
Y222 Phosphorylation
Y223 Phosphorylation
K235 Ubiquitination

Research Backgrounds

Function:

Converts guanidinoacetate to creatine, using S-adenosylmethionine as the methyl donor. Important in nervous system development.

Tissue Specificity:

Expressed in liver.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the class I-like SAM-binding methyltransferase superfamily. RMT2 methyltransferase family.

Research Fields

· Metabolism > Amino acid metabolism > Glycine, serine and threonine metabolism.

· Metabolism > Amino acid metabolism > Arginine and proline metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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