FHL2 Antibody - #DF13015

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Product: FHL2 Antibody
Catalog: DF13015
Description: Rabbit polyclonal antibody to FHL2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 32 kDa; 32kD(Calculated).
Uniprot: Q14192
RRID: AB_2845976

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(89%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
FHL2 Antibody detects endogenous levels of total FHL2.
RRID:
AB_2845976
Cite Format: Affinity Biosciences Cat# DF13015, RRID:AB_2845976.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AAG 11; AAG11; Aging associated gene 11; Down regulated in rhabdomyosarcoma LIM protein; Downregulated in rhabdomyosarcoma LIM protein; DRAL; FHL 2; FHL-2; Fhl2; FHL2 protein; FHL2_HUMAN; Four and a half LIM domain protein 2; Four and a half LIM domains 2; Four and a half LIM domains protein 2; KIAA0990; LIM domain protein DRAL; Skeletal muscle LIM protein 3; Skeletal muscle LIM-protein 3; SLIM 3; SLIM-3; SLIM3;

Immunogens

Immunogen:
Uniprot:

Q14192(FHL2_HUMAN) >>Visit HPA database.

Gene(ID):
FHL2(2274)
Expression:
Q14192 FHL2_HUMAN:

Expressed in skeletal muscle and heart.

Sequence:
MTERFDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGKDI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
89
Sheep
75
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14192 As Substrate

Site PTM Type Enzyme
S13 Phosphorylation
K17 Ubiquitination
Y27 Phosphorylation
Y56 Phosphorylation
K57 Acetylation
S74 Phosphorylation
K78 Sumoylation
K78 Ubiquitination
Y93 Phosphorylation
Y97 Phosphorylation
K100 Ubiquitination
K106 Ubiquitination
T107 Phosphorylation
T112 Phosphorylation
K118 Sumoylation
T138 Phosphorylation
K139 Sumoylation
K139 Ubiquitination
S140 Phosphorylation
K144 Sumoylation
K144 Ubiquitination
Y154 Phosphorylation
K156 Ubiquitination
K166 Ubiquitination
K167 Sumoylation
K167 Ubiquitination
T175 Phosphorylation
Y176 Phosphorylation
T189 Phosphorylation
K220 Sumoylation
K220 Ubiquitination
K235 Ubiquitination
Y236 Phosphorylation
S238 Phosphorylation
K252 Ubiquitination
K253 Sumoylation
K253 Ubiquitination
S255 Phosphorylation
K277 Acetylation
K277 Ubiquitination

Research Backgrounds

Function:

May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation. Negatively regulates the calcineurin/NFAT signaling pathway in cardiomyocytes.

Subcellular Location:

Cytoplasm. Nucleus. Cytoplasm>Myofibril>Sarcomere>Z line.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in skeletal muscle and heart.

Subunit Structure:

Interacts with ZNF638 and TTN/titin. Interacts with E4F1. Interacts with GRB7. Interacts with SIRT1 and FOXO1. Interacts with CEFIP. Interacts with calcineurin (By similarity). Interacts with FOXK1 (By similarity).

Family&Domains:

The third LIM zinc-binding mediates interaction with E4F1.

Research Fields

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

References

1). Cloning, Tissue Expression and Protein Localization of FHL2 Gene in Yaks. 畜牧兽医学报, 2023

Application: WB    Species: yak    Sample:

Fig. 1 Electrophoresis results of yak FHL2 PCR products

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