Exo70 Antibody - #DF12988

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Product: Exo70 Antibody
Catalog: DF12988
Description: Rabbit polyclonal antibody to Exo70
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog, Xenopus
Mol.Wt.: 74 kDa; 83kD(Calculated).
Uniprot: Q9UPT5
RRID: AB_2845949

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
Exo70 Antibody detects endogenous levels of total Exo70.
RRID:
AB_2845949
Cite Format: Affinity Biosciences Cat# DF12988, RRID:AB_2845949.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2 5 3p; DKFZp686J04253; EX070; EXO70; Exo70p; EXOC 1; EXOC 7; EXOC1; Exoc7; EXOC7_HUMAN; Exocyst complex component 7; Exocyst complex component Exo70; FLJ40965; FLJ46415; KIAA1067; Rexo70; YJL085W;

Immunogens

Immunogen:
Uniprot:

Q9UPT5(EXOC7_HUMAN) >>Visit HPA database.

Gene(ID):
EXOC7(23265)
Sequence:
MIPPQEASARRREIEDKLKQEEETLSFIRDSLEKSDQLTKNMVSILSSFESRLMKLENSIIPVHKQTENLQRLQENVEKTLSCLDHVISYYHVASDTEKIIREGPTGRLEEYLGSMAKIQKAVEYFQDNSPDSPELNKVKLLFERGKEALESEFRSLMTRHSKVVSPVLILDLISGDDDLEAQEDVTLEHLPESVLQDVIRISRWLVEYGRNQDFMNVYYQIRSSQLDRSIKGLKEHFHKSSSSSGVPYSPAIPNKRKDTPTKKPVKRPGTIRKAQNLLKQYSQHGLDGKKGGSNLIPLEGLLPCTPRGGLPGPWINAACVCAADISPGHEHDFRVKHLSEALNDKHGPLAGRDDMLDVETDAYIHCVSAFVKLAQSEYQLLADIIPEHHQKKTFDSLIQDALDGLMLEGENIVSAARKAIVRHDFSTVLTVFPILRHLKQTKPEFDQVLQGTAASTKNKLPGLITSMETIGAKALEDFADNIKNDPDKEYNMPKDGTVHELTSNAILFLQQLLDFQETAGAMLASQETSSSATSYSSEFSKRLLSTYICKVLGNLQLNLLSKSKVYEDPALSAIFLHNNYNYILKSLEKSELIQLVAVTQKTAERSYREHIEQQIQTYQRSWLKVTDYIAEKNLPVFQPGVKLRDKERQIIKERFKGFNDGLEELCKIQKAWAIPDTEQRDRIRQAQKTIVKETYGAFLQKFGSVPFTKNPEKYIKYGVEQVGDMIDRLFDTSA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
83
Zebrafish
58
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UPT5 As Substrate

Site PTM Type Enzyme
K19 Ubiquitination
K34 Ubiquitination
K40 Ubiquitination
K55 Ubiquitination
K65 Ubiquitination
Y90 Phosphorylation
K118 Ubiquitination
K121 Ubiquitination
Y125 Phosphorylation
S130 Phosphorylation
K138 Acetylation
K138 Ubiquitination
K140 Acetylation
K147 Acetylation
K147 Ubiquitination
S224 Phosphorylation
S225 Phosphorylation
S230 Phosphorylation
K240 Ubiquitination
Y249 Phosphorylation
S250 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
T271 Phosphorylation
K274 Ubiquitination
K280 Ubiquitination
S283 Phosphorylation
K337 Ubiquitination
S340 Phosphorylation
K443 Ubiquitination
T466 Phosphorylation
S467 Phosphorylation
K484 Ubiquitination
K563 Ubiquitination
K633 Ubiquitination
K653 Ubiquitination
K657 Ubiquitination
K668 Ubiquitination
K671 Ubiquitination
K714 Acetylation
K717 Acetylation

Research Backgrounds

Function:

Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. In adipocytes, plays a crucial role in targeting SLC2A4 vesicle to the plasma membrane in response to insulin, perhaps directing the vesicle to the precise site of fusion (By similarity).

Subcellular Location:

Cytoplasm>Cytosol. Cell membrane>Peripheral membrane protein. Midbody>Midbody ring.
Note: Translocates, as a preformed complex with EXOC3/SEC6 and EXOC4/SEC8, to the plasma membrane in response to insulin through the activation of ARHQ (By similarity). Colocalizes with CNTRL/centriolin at the midbody ring (PubMed:16213214).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

The exocyst complex is composed of EXOC1, EXOC2, EXOC3, EXOC4, EXOC5, EXOC6, EXOC7 and EXOC8. Interacts with ARHQ in a GTP-dependent manner. Interacts with RAB11FIP3 (By similarity).

Family&Domains:

The N-terminus is involved in SEC8 and ARHQ binding.

The C-terminus is required for translocation to the plasma membrane.

Belongs to the EXO70 family.

Research Fields

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

References

1). Exocyst controls exosome biogenesis via Rab11a. Molecular Therapy-Nucleic Acids, 2022 (PubMed: 35036064) [IF=8.8]

Application: IHC    Species:    Sample: LC tissue

Figure 1 Regulation of exosome release by exocysts in HNC (A and B) Sec10, Sec3, and Exo70 expression in LC tissue compared with that in Ctrl. (A) Representative images of immunohistochemistry staining. (B) Data summary of immunohistochemistry staining. LC, laryngocarcinoma tissue; Ctrl, adjacent normal tissues. ∗∗p < 0.01, ∗∗∗p < 0.001 by t test. Scale bar, 50 μm. (C and D) Sec10, Sec3, and Exo70 expression in HN4 and NP69 cells. (C) Representative images of western blotting. (D) The summary data of western blotting compared with NP69 cells. (E and F) Representative NTA traces and quantification of exosomes after knockdown of the expression of exocyst subunits by transfecting HN4 (E) and CNE2 (F) cells with specific small interfering (si)RNA for Sec10, Sec3, or Exo70 protein. ∗p < 0.05, ∗∗p < 0.05, ∗∗∗p < 0.001 by t test.

Application: WB    Species: Human    Sample: HN4 and NP69 cells

Figure 1 Regulation of exosome release by exocysts in HNC (A and B) Sec10, Sec3, and Exo70 expression in LC tissue compared with that in Ctrl. (A) Representative images of immunohistochemistry staining. (B) Data summary of immunohistochemistry staining. LC, laryngocarcinoma tissue; Ctrl, adjacent normal tissues. ∗∗p < 0.01, ∗∗∗p < 0.001 by t test. Scale bar, 50 μm. (C and D) Sec10, Sec3, and Exo70 expression in HN4 and NP69 cells. (C) Representative images of western blotting. (D) The summary data of western blotting compared with NP69 cells. (E and F) Representative NTA traces and quantification of exosomes after knockdown of the expression of exocyst subunits by transfecting HN4 (E) and CNE2 (F) cells with specific small interfering (si)RNA for Sec10, Sec3, or Exo70 protein. ∗p < 0.05, ∗∗p < 0.05, ∗∗∗p < 0.001 by t test.

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