Product: ERGIC2 Antibody
Catalog: DF12982
Description: Rabbit polyclonal antibody to ERGIC2
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 45 kDa; 43kD(Calculated).
Uniprot: Q96RQ1
RRID: AB_2845943

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(92%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
ERGIC2 Antibody detects endogenous levels of total ERGIC2.
RRID:
AB_2845943
Cite Format: Affinity Biosciences Cat# DF12982, RRID:AB_2845943.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

cd002; CD14 protein; CDA14; Endoplasmic reticulum Golgi intermediate compartment protein 2; Endoplasmic reticulum-Golgi intermediate compartment protein 2; ERGI2_HUMAN; ERGIC 2; ERGIC and golgi 2; ergic2; Erv41; MGC111152; PTX1; PTX1 protein;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q96RQ1 ERGI2_HUMAN:

Ubiquitously expressed.

Sequence:
MRRLNRKKTLSLVKELDAFPKVPESYVETSASGGTVSLIAFTTMALLTIMEFSVYQDTWMKYEYEVDKDFSSKLRINIDITVAMKCQYVGADVLDLAETMVASADGLVYEPTVFDLSPQQKEWQRMLQLIQSRLQEEHSLQDVIFKSAFKSTSTALPPREDDSSQSPNACRIHGHLYVNKVAGNFHITVGKAIPHPRGHAHLAALVNHESYNFSHRIDHLSFGELVPAIINPLDGTEKIAIDHNQMFQYFITVVPTKLHTYKISADTHQFSVTERERIINHAAGSHGVSGIFMKYDLSSLMVTVTEEHMPFWQFFVRLCGIVGGIFSTTGMLHGIGKFIVEIICCRFRLGSYKPVNSVPFEDGHTDNHLPLLENNTH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Pig
92
Bovine
92
Sheep
92
Chicken
75
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96RQ1 As Substrate

Site PTM Type Enzyme
T9 Phosphorylation
S11 Phosphorylation
K14 Ubiquitination
K68 Ubiquitination
Y109 Phosphorylation
K146 Ubiquitination
K150 Ubiquitination
T154 O-Glycosylation
Y177 Phosphorylation
K262 Ubiquitination

Research Backgrounds

Function:

Possible role in transport between endoplasmic reticulum and Golgi.

Subcellular Location:

Endoplasmic reticulum-Golgi intermediate compartment membrane>Multi-pass membrane protein. Golgi apparatus>cis-Golgi network membrane>Multi-pass membrane protein. Endoplasmic reticulum membrane>Multi-pass membrane protein. Cytoplasm. Nucleus.
Note: Cycles between the endoplasmic reticulum and the Golgi. According to a report, localizes to the nucleus (PubMed:11445006). Another report shows a partial localization in the nucleus (PubMed:17980171).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

May interact with EEF1A1.

Family&Domains:

Belongs to the ERGIC family.

Restrictive clause

 

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