ENY2 Antibody | Affinity Biosciences

WB
Cites

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Western blot analysis of extracts from Jurkat cells, using ENY2 Antibody.

Product:	ENY2 Antibody
Catalog:	DF12979
Description:	Rabbit polyclonal antibody to ENY2
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	10 kDa; 12kD(Calculated).
Uniprot:	Q9NPA8
RRID:	AB_2845940

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(91%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

ENY2 Antibody detects endogenous levels of total ENY2.

RRID:

AB_2845940
Cite Format: Affinity Biosciences Cat# DF12979, RRID:AB_2845940.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

1810057B09Rik; 6720481I12; DC6; e(y)2; Enhancer of yellow 2 transcription factor homolog; eny2; ENY2_HUMAN; Ey2;

Immunogens

Immunogen:

Uniprot:

Q9NPA8(ENY2_HUMAN) >>Visit HPA database.

Gene(ID):

ENY2(56943)

Sequence:

Q9NPA8 ENY2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVVSKMNKDAQMRAAINQKLIETGERERLKELLRAKLIECGWKDQLKAHCKEVIKEKGLEHVTVDDLVAEITPKGRALVPDSVKKELLQRIRTFLAQHASL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Dog

100

Xenopus

100

Chicken

100

Rabbit

100

Zebrafish

Sheep

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NPA8 As Substrate

Q9NPA8

Site	PTM Type	Source
S4	Phosphorylation	Uniprot
K8	Ubiquitination	Uniprot
K19	Acetylation	Uniprot
K19	Ubiquitination	Uniprot
K30	Ubiquitination	Uniprot
K36	Ubiquitination	Uniprot
C40	S-Nitrosylation	Uniprot
K43	Acetylation	Uniprot
K43	Ubiquitination	Uniprot
K57	Ubiquitination	Uniprot
T72	Phosphorylation	Uniprot
K74	Ubiquitination	Uniprot
S82	Phosphorylation	Uniprot
S100	Phosphorylation	Uniprot

Research Backgrounds

Q9NPA8_ENY2_HUMAN

Function:

Involved in mRNA export coupled transcription activation by association with both the TREX-2 and the SAGA complexes. The transcription regulatory histone acetylation (HAT) complex SAGA is a multiprotein complex that activates transcription by remodeling chromatin and mediating histone acetylation and deubiquitination. Within the SAGA complex, participates in a subcomplex that specifically deubiquitinates both histones H2A and H2B. The SAGA complex is recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation. As a component of the TREX-2 complex, involved in the export of mRNAs to the cytoplasm through the nuclear pores.

Subcellular Location:

Nucleus>Nucleoplasm. Nucleus>Nuclear pore complex.
Note: Localization at the nuclear pore complex requires NUP153 and TPR.

Subunit Structure:

Component of the nuclear pore complex (NPC)-associated TREX-2 complex (transcription and export complex 2), composed of at least ENY2, the isoform GANP of the MCM3AP gene, PCID2, SEM1, and either centrin CETN2 or CETN3. TREX-2 contains 2 ENY2 chains. The TREX-2 complex also associates with ALYREF/ALY and with the nucleoporin NUP153. Component of some SAGA transcription coactivator-HAT complexes, at least composed of ATXN7, ATXN7L3, ENY2, GCN5L2, SUPT3H/SPT3, TAF10, TRRAP and USP22. Within the SAGA complex, ENY2, ATXN7, ATXN7L3, and USP22 form an additional subcomplex of SAGA called the DUB module (deubiquitination module). Interacts with RNA polymerase II subunit POLR2A. Interacts with ATXN7L3B.

Family&Domains:

Belongs to the ENY2 family.

References

1). TMT-based quantitative proteomics analysis reveals the attenuated replication mechanism of Newcastle disease virus caused by nuclear localization signal mutation in viral matrix protein. Virulence, 2020 (PubMed: 32420802) [IF=5.2]

Application: WB Species: Mouse Sample: BSR-T7/5 cells

Figure 7. rSS1GFP infection inhibits host cell transcription, RNA processing and modification. (A) The heatmap of representative 20 DEPs related to “Transcription” during rSS1GFP and rSS1GFP-M/NLSm infection at 12 and 24 h. (B) The protein-protein interactions of the DEPs related to “Transcription” are analyzed by the STRING software. A red line indicates the presence of fusion evidence; a blue line indicates co-occurrence evidence; a light blue line indicates database evidence; a purple line indicates experimental evidence; a green line indicates neighborhood evidence; a black line indicates co-expression evidence. (C) The heatmap of representative 20 DEPs related to “RNA processing and modification” during rSS1GFP and rSS1GFP-M/NLSm infection at 12 and 24 h. (D) The protein-protein interactions of the DEPs related to “RNA processing and modification” are analyzed by the STRING software. (E) The mRNA expression levels of four selected DEP genes in BSR-T7/5 cells infected with rSS1GFP and rSS1GFP-M/NLSm were verified by qRT-PCR. (F) The protein expression levels of four DEPs in BSR-T7/5 cells infected with rSS1GFP and rSS1GFP-M/NLSm were examined by Western blotting. The relative expression levels of four DEPs were compared with the control GAPDH expression. Error bars represent standard deviations (mean ± SD) (*P < 0.05; **P < 0.01; ***P < 0.001 compared to the value of rSS1GFP-M/NLSm).

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ENY2 Antibody - #DF12979