ECH1 Antibody - #DF12970

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Product: ECH1 Antibody
Catalog: DF12970
Description: Rabbit polyclonal antibody to ECH1
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Sheep, Rabbit, Chicken
Mol.Wt.: 30 kDa; 36kD(Calculated).
Uniprot: Q13011
RRID: AB_2845931

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(88%), Zebrafish(88%), Bovine(88%), Sheep(88%), Rabbit(88%), Chicken(88%)
Clonality:
Polyclonal
Specificity:
ECH1 Antibody detects endogenous levels of total ECH1.
RRID:
AB_2845931
Cite Format: Affinity Biosciences Cat# DF12970, RRID:AB_2845931.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

4)-dienoyl-CoA isomerase; 5)-Delta(2; Delta(3 5) Delta(2 4) dienoyl CoA isomerase; Delta(3 5) Delta(2 4) dienoyl CoA isomerase mitochondrial; Delta(3; Delta3 5 delta2 4 dienoyl CoA isomerase; Dienoyl CoA isomerase; ECH 1; Ech1; ECH1_HUMAN; Enoyl Coenzyme A hydratase 1; Enoyl Coenzyme A hydratase 1 peroxisomal; HPXEL; mitochondrial; MS1034; Peroxisomal enoyl CoA hydratase 1; Peroxisomal enoyl coenzyme A hydratase like protein;

Immunogens

Immunogen:
Uniprot:

Q13011(ECH1_HUMAN) >>Visit HPA database.

Gene(ID):
ECH1(1891)
Sequence:
MAAGIVASRRLRDLLTRRLTGSNYPGLSISLRLTGSSAQEEASGVALGEAPDHSYESLRVTSAQKHVLHVQLNRPNKRNAMNKVFWREMVECFNKISRDADCRAVVISGAGKMFTAGIDLMDMASDILQPKGDDVARISWYLRDIITRYQETFNVIERCPKPVIAAVHGGCIGGGVDLVTACDIRYCAQDAFFQVKEVDVGLAADVGTLQRLPKVIGNQSLVNELAFTARKMMADEALGSGLVSRVFPDKEVMLDAALALAAEISSKSPVAVQSTKVNLLYSRDHSVAESLNYVASWNMSMLQTQDLVKSVQATTENKELKTVTFSKL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
88
Bovine
88
Sheep
88
Zebrafish
88
Chicken
88
Rabbit
88
Horse
75
Xenopus
75
Dog
63
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13011 As Substrate

Site PTM Type Enzyme
T20 Phosphorylation
S22 Phosphorylation
S28 Phosphorylation
S30 Phosphorylation
S37 Phosphorylation
S54 Phosphorylation
K65 Acetylation
K65 Ubiquitination
K77 Acetylation
K83 Acetylation
K83 Ubiquitination
K95 Acetylation
S108 Phosphorylation
S139 Phosphorylation
Y141 Phosphorylation
K214 Ubiquitination
S220 Phosphorylation
T228 Phosphorylation
K231 Ubiquitination
S240 Phosphorylation
S244 Phosphorylation
S265 Phosphorylation
K267 Acetylation
S268 Phosphorylation
K276 Ubiquitination
Y281 Phosphorylation
S282 Phosphorylation
K318 Acetylation
K318 Ubiquitination
K321 Ubiquitination
K327 Acetylation
K327 Ubiquitination

Research Backgrounds

Function:

Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA.

Subcellular Location:

Mitochondrion. Peroxisome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homohexamer.

Family&Domains:

Belongs to the enoyl-CoA hydratase/isomerase family.

Research Fields

· Cellular Processes > Transport and catabolism > Peroxisome.   (View pathway)

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