DYNLL2 Antibody | Affinity Biosciences

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Western blot analysis of extracts from A431 cells, using DYNLL2 Antibody.

Product:	DYNLL2 Antibody
Catalog:	DF12966
Description:	Rabbit polyclonal antibody to DYNLL2
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	10 kDa; 10kD(Calculated).
Uniprot:	Q96FJ2
RRID:	AB_2845927

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

DYNLL2 Antibody detects endogenous levels of total DYNLL2.

RRID:

AB_2845927
Cite Format: Affinity Biosciences Cat# DF12966, RRID:AB_2845927.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

8 kDa dynein light chain b; C87222; cytoplasmic; Dlc2; DLC8b; DNCL1B; DYL2_HUMAN; Dynein light chain 2; Dynein light chain 2, cytoplasmic; Dynein light chain LC8 type 2; Dynein light chain LC8-type 2; Dynll2; MGC17810; MGC72334;

Immunogens

Immunogen:

Uniprot:

Q96FJ2(DYL2_HUMAN) >>Visit HPA database.

Gene(ID):

DYNLL2(140735)

Sequence:

Q96FJ2 DYL2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSDRKAVIKNADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Chicken

100

Rabbit

100

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96FJ2 As Substrate

Q96FJ2

Site	PTM Type	Source
K5	Acetylation	Uniprot
S14	Phosphorylation	Uniprot
K31	Ubiquitination	Uniprot
Y32	Phosphorylation	Uniprot
K36	Acetylation	Uniprot
K36	Ubiquitination	Uniprot
Y41	Phosphorylation	Uniprot
K49	Methylation	Uniprot
K49	Sumoylation	Uniprot
K49	Ubiquitination	Uniprot
S64	Phosphorylation	Uniprot
Y65	Phosphorylation	Uniprot
T67	Phosphorylation	Uniprot
S88	Phosphorylation	Uniprot

Research Backgrounds

Q96FJ2_DYL2_HUMAN

Function:

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures (By similarity).

Subcellular Location:

Cytoplasm>Cytoskeleton.

Subunit Structure:

Homodimer. The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits which present intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. Dynein ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. Interacts with DYNC1I1. Interacts with BMF. Component of the myosin V motor complex. Interacts with BCAS1. Interacts with Basson/BSN.

Family&Domains:

Belongs to the dynein light chain family.

Research Fields

· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.

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DYNLL2 Antibody - #DF12966