DNM2 Antibody - #DF12953

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Product: DNM2 Antibody
Catalog: DF12953
Description: Rabbit polyclonal antibody to DNM2
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 100 kDa; 98kD(Calculated).
Uniprot: P50570
RRID: AB_2845914

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(92%), Rabbit(92%), Dog(92%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
DNM2 Antibody detects endogenous levels of total DNM2.
RRID:
AB_2845914
Cite Format: Affinity Biosciences Cat# DF12953, RRID:AB_2845914.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CMT2M; CMTDI1; CMTDIB; DI CMTB; Dnm2; DYN II; DYN2; DYN2_HUMAN; Dynamin II; Dynamin-2; Dynamin2; DynaminII; DYNII;

Immunogens

Immunogen:
Uniprot:

P50570(DYN2_HUMAN) >>Visit HPA database.

Gene(ID):
DNM2(1785)
Expression:
P50570 DYN2_HUMAN:

Ubiquitously expressed.

Sequence:
MGNRGMEELIPLVNKLQDAFSSIGQSCHLDLPQIAVVGGQSAGKSSVLENFVGRDFLPRGSGIVTRRPLILQLIFSKTEHAEFLHCKSKKFTDFDEVRQEIEAETDRVTGTNKGISPVPINLRVYSPHVLNLTLIDLPGITKVPVGDQPPDIEYQIKDMILQFISRESSLILAVTPANMDLANSDALKLAKEVDPQGLRTIGVITKLDLMDEGTDARDVLENKLLPLRRGYIGVVNRSQKDIEGKKDIRAALAAERKFFLSHPAYRHMADRMGTPHLQKTLNQQLTNHIRESLPALRSKLQSQLLSLEKEVEEYKNFRPDDPTRKTKALLQMVQQFGVDFEKRIEGSGDQVDTLELSGGARINRIFHERFPFELVKMEFDEKDLRREISYAIKNIHGVRTGLFTPDLAFEAIVKKQVVKLKEPCLKCVDLVIQELINTVRQCTSKLSSYPRLREETERIVTTYIREREGRTKDQILLLIDIEQSYINTNHEDFIGFANAQQRSTQLNKKRAIPNQGEILVIRRGWLTINNISLMKGGSKEYWFVLTAESLSWYKDEEEKEKKYMLPLDNLKIRDVEKGFMSNKHVFAIFNTEQRNVYKDLRQIELACDSQEDVDSWKASFLRAGVYPEKDQAENEDGAQENTFSMDPQLERQVETIRNLVDSYVAIINKSIRDLMPKTIMHLMINNTKAFIHHELLAYLYSSADQSSLMEESADQAQRRDDMLRMYHALKEALNIIGDISTSTVSTPVPPPVDDTWLQSASSHSPTPQRRPVSSIHPPGRPPAVRGPTPGPPLIPVPVGAAASFSAPPIPSRPGPQSVFANSDLFPAPPQIPSRPVRIPPGIPPGVPSRRPPAAPSRPTIIRPAEPSLLD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
92
Dog
92
Xenopus
92
Rabbit
92
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P50570 As Substrate

Site PTM Type Enzyme
C27 S-Nitrosylation
S46 Phosphorylation
S61 Phosphorylation
C86 S-Nitrosylation
K87 Ubiquitination
K90 Ubiquitination
K113 Ubiquitination
S116 Phosphorylation
Y125 Phosphorylation
S126 Phosphorylation
K191 Ubiquitination
T200 Phosphorylation
K206 Ubiquitination
T214 Phosphorylation
R217 Methylation
K223 Ubiquitination
Y231 Phosphorylation
K240 Acetylation
K257 Ubiquitination
T280 Phosphorylation
T286 Phosphorylation
S292 Phosphorylation
K299 Acetylation
K299 Ubiquitination
K315 Ubiquitination
S347 Phosphorylation
T353 Phosphorylation
S357 Phosphorylation
K376 Ubiquitination
K382 Ubiquitination
K393 Ubiquitination
T400 Phosphorylation
T404 Phosphorylation
K414 Ubiquitination
Y449 Phosphorylation
Y463 Phosphorylation
T527 Phosphorylation
S532 Phosphorylation
Y563 Phosphorylation
K577 Ubiquitination
S581 Phosphorylation
T591 Phosphorylation
Y597 Phosphorylation
K598 Acetylation
K598 Ubiquitination
C607 S-Nitrosylation
K617 Ubiquitination
S644 Phosphorylation
S662 Phosphorylation
Y663 Phosphorylation
K669 Ubiquitination
Y726 Phosphorylation
T755 Phosphorylation
S759 Phosphorylation
S761 Phosphorylation
S762 Phosphorylation
S764 Phosphorylation P24941 (CDK2) , P06493 (CDK1)
T766 Phosphorylation P06493 (CDK1)
R785 Methylation
S848 Phosphorylation
R850 Methylation
R857 Methylation
R862 Methylation

Research Backgrounds

Function:

Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity). Plays an important role in vesicular trafficking processes, in particular endocytosis. Involved in cytokinesis. Regulates maturation of apoptotic cell corpse-containing phagosomes by recruiting PIK3C3 to the phagosome membrane (By similarity).

PTMs:

Phosphorylation at Ser-764 by CDK1 is greatly increased upon mitotic entry. It regulates cytokinesis downstream of calcineurin, and does not affect clathrin-mediated endocytosis. Dephosphorylated by calcineurin/PP2 (By similarity). Phosphorylated on tyrosine residues after activation of SRC (By similarity).

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton. Cell junction. Membrane>Clathrin-coated pit. Cell junction>Synapse>Postsynaptic density. Cell junction>Synapse. Midbody. Cell projection>Phagocytic cup. Cytoplasmic vesicle>Phagosome membrane>Peripheral membrane protein.
Note: Colocalizes with CTTN at the basis of filopodia in hippocampus neuron growth zones (By similarity). Microtubule-associated. Also found in the postsynaptic density of neuronal cells. Co-localizes with PIK3C3 and RAB5A to the nascent phagosome (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Interacts with MYOF (By similarity). Interacts with CTTN and ACTN1 (By similarity). Interacts with SHANK1, SHANK2, SH3BP4 and NOSTRIN. Interacts with SNX9. Interacts with SNX33 (via SH3 domain). Interacts with MYO1E (via SH3 domain). Interacts with PSTPIP1. Interacts with CTNND2. May interact with PIK3C3 (By similarity). May be a component of a complex composed of RAB5A (in GDP-bound form), DYN2 and PIK3C3 (By similarity). Interacts with BIN1.

Family&Domains:

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

· Organismal Systems > Nervous system > Synaptic vesicle cycle.

· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.

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