DHX15 Antibody - #DF12939

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Product: DHX15 Antibody
Catalog: DF12939
Description: Rabbit polyclonal antibody to DHX15
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 91 kDa; 91kD(Calculated).
Uniprot: O43143
RRID: AB_2845900

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
DHX15 Antibody detects endogenous levels of total DHX15.
RRID:
AB_2845900
Cite Format: Affinity Biosciences Cat# DF12939, RRID:AB_2845900.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATP dependent RNA helicase #46; ATP-dependent RNA helicase #46; DBP1; DDX15; DEADH box 15; DEAH (Asp Glu Ala His) box polypeptide 15; DEAH box polypeptide 15; DEAH box protein 15; DHX15; DHX15_HUMAN; HRH2; Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15; PRP43; PrPp43p; RNA helicase 2;

Immunogens

Immunogen:
Uniprot:

O43143(DHX15_HUMAN) >>Visit HPA database.

Gene(ID):
DHX15(1665)
Expression:
O43143 DHX15_HUMAN:

Ubiquitous.

Sequence:
MSKRHRLDLGEDYPSGKKRAGTDGKDRDRDRDREDRSKDRDRERDRGDREREREKEKEKELRASTNAMLISAGLPPLKASHSAHSTHSAHSTHSTHSAHSTHAGHAGHTSLPQCINPFTNLPHTPRYYDILKKRLQLPVWEYKDRFTDILVRHQSFVLVGETGSGKTTQIPQWCVEYMRSLPGPKRGVACTQPRRVAAMSVAQRVADEMDVMLGQEVGYSIRFEDCSSAKTILKYMTDGMLLREAMNDPLLERYGVIILDEAHERTLATDILMGVLKEVVRQRSDLKVIVMSATLDAGKFQIYFDNCPLLTIPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHMCEEEEGDLLLFLTGQEEIDEACKRIKREVDDLGPEVGDIKIIPLYSTLPPQQQQRIFEPPPPKKQNGAIGRKVVVSTNIAETSLTIDGVVFVIDPGFAKQKVYNPRIRVESLLVTAISKASAQQRAGRAGRTRPGKCFRLYTEKAYKTEMQDNTYPEILRSNLGSVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNYLAALNDDGDLTELGSMMAEFPLDPQLAKMVIASCDYNCSNEVLSITAMLSVPQCFVRPTEAKKAADEAKMRFAHIDGDHLTLLNVYHAFKQNHESVQWCYDNFINYRSLMSADNVRQQLSRIMDRFNLPRRSTDFTSRDYYINIRKALVTGYFMQVAHLERTGHYLTVKDNQVVQLHPSTVLDHKPEWVLYNEFVLTTKNYIRTCTDIKPEWLVKIAPQYYDMSNFPQCEAKRQLDRIIAKLQSKEYSQY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
88
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43143 As Substrate

Site PTM Type Enzyme
K3 Acetylation
Y13 Phosphorylation
S15 Phosphorylation
K17 Acetylation
K17 Ubiquitination
K18 Acetylation
K18 Methylation
R42 Methylation
R44 Methylation
S71 Phosphorylation
Y128 Phosphorylation
K132 Acetylation
K132 Ubiquitination
Y142 Phosphorylation
K143 Ubiquitination
S155 Phosphorylation
T162 Phosphorylation
S164 Phosphorylation
K166 Acetylation
K185 Acetylation
C190 S-Nitrosylation
K230 Ubiquitination
K234 Ubiquitination
Y254 Phosphorylation
T266 Phosphorylation
S292 Phosphorylation
T324 Phosphorylation
Y331 Phosphorylation
K370 Ubiquitination
K384 Ubiquitination
K407 Ubiquitination
K445 Acetylation
K445 Ubiquitination
K463 Ubiquitination
T476 Phosphorylation
K480 Ubiquitination
K488 Acetylation
K488 Ubiquitination
Y490 Phosphorylation
K491 Sumoylation
K491 Ubiquitination
Y499 Phosphorylation
S505 Phosphorylation
S509 Phosphorylation
K515 Ubiquitination
K516 Ubiquitination
K614 Ubiquitination
Y631 Phosphorylation
S653 Phosphorylation
R670 Methylation
Y686 Phosphorylation
T695 Phosphorylation
Y710 Phosphorylation
K754 Acetylation
K754 Ubiquitination
K760 Ubiquitination
K777 Ubiquitination
K786 Acetylation
K786 Ubiquitination
K790 Ubiquitination
Y792 Phosphorylation
S793 Phosphorylation
Y795 Phosphorylation

Research Backgrounds

Function:

Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. In cooperation with TFIP11 seem to be involved in the transition of the U2, U5 and U6 snRNP-containing IL complex to the snRNP-free IS complex leading to efficient debranching and turnover of excised introns.

Subcellular Location:

Nucleus. Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with SSB/La. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Identified in the Intron Large (IL) complex, a post-mRNA release spliceosomal complex containing the excised intron, U2, U5 and U6 snRNPs, and splicing factors; the association may be transient. Interacts with TFIP11; indicative for a recruitment to the IL complex. Interacts with GPATCH2.

Family&Domains:

Belongs to the DEAD box helicase family. DEAH subfamily. DDX15/PRP43 sub-subfamily.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

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