DDX6 Antibody - #DF12937

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Product: DDX6 Antibody
Catalog: DF12937
Description: Rabbit polyclonal antibody to DDX6
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 54 kDa; 54kD(Calculated).
Uniprot: P26196
RRID: AB_2845898

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
DDX6 Antibody detects endogenous levels of total DDX6.
RRID:
AB_2845898
Cite Format: Affinity Biosciences Cat# DF12937, RRID:AB_2845898.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATP dependent RNA helicase DDX6; ATP dependent RNA helicase p54; DDX6; DEAD (Asp Glu Ala Asp) box polypeptide 6; DEAD box 6; DEAD box protein 6; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 6 (RNA helicase 54kD); FLJ36338; HLR2; Oncogene RCK; P54; Probable ATP-dependent RNA helicase DDX6; RCK;

Immunogens

Immunogen:
Uniprot:

P26196(DDX6_HUMAN) >>Visit HPA database.

Gene(ID):
DDX6(1656)
Expression:
P26196 DDX6_HUMAN:

Abundantly expressed in most tissues.

Sequence:
MSTARTENPVIMGLSSQNGQLRGPVKPTGGPGGGGTQTQQQMNQLKNTNTINNGTQQQAQSMTTTIKPGDDWKKTLKLPPKDLRIKTSDVTSTKGNEFEDYCLKRELLMGIFEMGWEKPSPIQEESIPIALSGRDILARAKNGTGKSGAYLIPLLERLDLKKDNIQAMVIVPTRELALQVSQICIQVSKHMGGAKVMATTGGTNLRDDIMRLDDTVHVVIATPGRILDLIKKGVAKVDHVQMIVLDEADKLLSQDFVQIMEDIILTLPKNRQILLYSATFPLSVQKFMNSHLQKPYEINLMEELTLKGVTQYYAYVTERQKVHCLNTLFSRLQINQSIIFCNSSQRVELLAKKISQLGYSCFYIHAKMRQEHRNRVFHDFRNGLCRNLVCTDLFTRGIDIQAVNVVINFDFPKLAETYLHRIGRSGRFGHLGLAINLITYDDRFNLKSIEEQLGTEIKPIPSNIDKSLYVAEYHSEPVEDEKP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
92
Zebrafish
92
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P26196 As Substrate

Site PTM Type Enzyme
S15 Phosphorylation
S16 Phosphorylation
K26 Ubiquitination
T36 Phosphorylation
T38 Phosphorylation
T64 Phosphorylation
K67 Acetylation
K67 Ubiquitination
K73 Acetylation
K73 Ubiquitination
K74 Acetylation
T75 Phosphorylation
K81 Ubiquitination
K86 Ubiquitination
T87 Phosphorylation
K94 Ubiquitination
K104 Acetylation
K104 Ubiquitination
K146 Ubiquitination
S147 Phosphorylation
K162 Ubiquitination
K195 Ubiquitination
T199 Phosphorylation
K231 Ubiquitination
Y312 Phosphorylation
Y313 Phosphorylation
C324 S-Nitrosylation
T327 Phosphorylation
S330 Phosphorylation
R375 Methylation
T439 Phosphorylation
Y440 Phosphorylation
K447 Ubiquitination
K458 Ubiquitination
S462 Phosphorylation
K466 Ubiquitination
Y473 Phosphorylation
S475 Phosphorylation
K482 Ubiquitination

Research Backgrounds

Function:

In the process of mRNA degradation, plays a role in mRNA decapping. Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts.

PTMs:

Sumoylated.

Subcellular Location:

Cytoplasm>P-body. Cytoplasm. Nucleus.
Note: Upon cellular stress, relocalizes to stress granules (PubMed:26184334).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Abundantly expressed in most tissues.

Subunit Structure:

Forms a complex with DCP1A, DCP2, EDC3 and EDC4/HEDLS. Interacts with LIMD1, WTIP and AJUBA. Interacts with APOBEC3G in an RNA-dependent manner. Interacts with RC3H1 (By similarity). Interacts with ATXN2L. Interacts with MCRIP1. Interacts with MCRIP2. Interacts with NUFIP2. Interacts with TRIM71 (via NHL repeats) in an RNA-dependent manner.

Family&Domains:

Belongs to the DEAD box helicase family. DDX6/DHH1 subfamily.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.

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