Product: DDA1 Antibody
Catalog: DF12930
Description: Rabbit polyclonal antibody to DDA1
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Dog, Chicken
Mol.Wt.: 12 kDa; 12kD(Calculated).
Uniprot: Q9BW61
RRID: AB_2845891

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Dog(100%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
DDA1 Antibody detects endogenous levels of total DDA1.
RRID:
AB_2845891
Cite Format: Affinity Biosciences Cat# DF12930, RRID:AB_2845891.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C19orf58; Cross immune reaction antigen PCIA1; DDA1; DDA1_HUMAN; DET1 and DDB1 associated 1; DET1 and DDB1 associated protein 1; DET1- and DDB1-associated protein 1; MGC2594; PCIA-1; PCIA1; Placenta cross immune reaction antigen 1; Placenta cross-immune reaction antigen 1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MADFLKGLPVYNKSNFSRFHADSVCKASNRRPSVYLPTREYPSEQIIVTEKTNILLRYLHQQWDKKNAAKKRDQEQVELEGESSAPPRKVARTDSPDMHEDT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
83
Xenopus
75
Zebrafish
75
Horse
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BW61 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K6 Ubiquitination
K13 Ubiquitination
S23 Phosphorylation
C25 S-Nitrosylation
K26 Ubiquitination
S28 Phosphorylation
S33 Phosphorylation Q96GD4 (AURKB)
Y35 Phosphorylation
T49 Phosphorylation
T52 Phosphorylation
K65 Acetylation
K65 Ubiquitination
K71 Ubiquitination
T93 Phosphorylation
S95 Phosphorylation

Research Backgrounds

Function:

May be involved in ubiquitination and subsequent proteasomal degradation of target proteins. Component of the DDD-E2 complexes which may provide a platform for interaction with CUL4A and WD repeat proteins.

Subunit Structure:

Part of the DDD core complex containing DET1, DDA1 and DDB1. The DDD core complex recruits a specific UBE2E enzyme, such as UBE2E1, UBE2E2 UBE2E3, to form specific DDD-E2 complexes.

Family&Domains:

Belongs to the DDA1 family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.