CYFIP2 Antibody - #DF12927

(2)  
Product: CYFIP2 Antibody
Catalog: DF12927
Description: Rabbit polyclonal antibody to CYFIP2
Application: WB
Reactivity: Human, Mouse, Monkey
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 130 kDa; 148kD(Calculated).
Uniprot: Q96F07
RRID: AB_2845888

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Monkey
Prediction:
Pig(100%), Zebrafish(100%), Bovine(91%), Horse(100%), Sheep(91%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
CYFIP2 Antibody detects endogenous levels of total CYFIP2.
RRID:
AB_2845888
Cite Format: Affinity Biosciences Cat# DF12927, RRID:AB_2845888.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CYFP2; cytoplasmic FMR1 interacting protein 2; KIAA1168; p53 inducible protein; p53-inducible protein 121; PIR121;

Immunogens

Immunogen:
Uniprot:

Q96F07(CYFP2_HUMAN) >>Visit HPA database.

Gene(ID):
CYFIP2(26999)
Expression:
Q96F07 CYFP2_HUMAN:

Expressed in T-cells. Increased expression is observed in CD4(+) T-lymphocytes from patients with multiple sclerosis (at protein level).

Sequence:
MTTHVTLEDALSNVDLLEELPLPDQQPCIEPPPSSIMYQANFDTNFEDRNAFVTGIARYIEQATVHSSMNEMLEEGHEYAVMLYTWRSCSRAIPQVKCNEQPNRVEIYEKTVEVLEPEVTKLMKFMYFQRKAIERFCSEVKRLCHAERRKDFVSEAYLLTLGKFINMFAVLDELKNMKCSVKNDHSAYKRAAQFLRKMADPQSIQESQNLSMFLANHNRITQCLHQQLEVIPGYEELLADIVNICVDYYENKMYLTPSEKHMLLKVMGFGLYLMDGNVSNIYKLDAKKRINLSKIDKFFKQLQVVPLFGDMQIELARYIKTSAHYEENKSKWTCTQSSISPQYNICEQMVQIRDDHIRFISELARYSNSEVVTGSGLDSQKSDEEYRELFDLALRGLQLLSKWSAHVMEVYSWKLVHPTDKFCNKDCPGTAEEYERATRYNYTSEEKFAFVEVIAMIKGLQVLMGRMESVFNQAIRNTIYAALQDFAQVTLREPLRQAVRKKKNVLISVLQAIRKTICDWEGGREPPNDPCLRGEKDPKGGFDIKVPRRAVGPSSTQACQWSPRALFHPTGGTQGRRGCRSLLYMVRTMLESLIADKSGSKKTLRSSLDGPIVLAIEDFHKQSFFFTHLLNISEALQQCCDLSQLWFREFFLELTMGRRIQFPIEMSMPWILTDHILETKEPSMMEYVLYPLDLYNDSAYYALTKFKKQFLYDEIEAEVNLCFDQFVYKLADQIFAYYKAMAGSVLLDKRFRAECKNYGVIIPYPPSNRYETLLKQRHVQLLGRSIDLNRLITQRISAAMYKSLDQAISRFESEDLTSIVELEWLLEINRLTHRLLCKHMTLDSFDAMFREANHNVSAPYGRITLHVFWELNFDFLPNYCYNGSTNRFVRTAIPFTQEPQRDKPANVQPYYLYGSKPLNIAYSHIYSSYRNFVGPPHFKTICRLLGYQGIAVVMEELLKIVKSLLQGTILQYVKTLIEVMPKICRLPRHEYGSPGILEFFHHQLKDIIEYAELKTDVFQSLREVGNAILFCLLIEQALSQEEVCDLLHAAPFQNILPRVYIKEGERLEVRMKRLEAKYAPLHLVPLIERLGTPQQIAIAREGDLLTKERLCCGLSMFEVILTRIRSYLQDPIWRGPPPTNGVMHVDECVEFHRLWSAMQFVYCIPVGTNEFTAEQCFGDGLNWAGCSIIVLLGQQRRFDLFDFCYHLLKVQRQDGKDEIIKNVPLKKMADRIRKYQILNNEVFAILNKYMKSVETDSSTVEHVRCFQPPIHQSLATTC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Bovine
91
Sheep
91
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96F07 As Substrate

Site PTM Type Enzyme
K97 Ubiquitination
C98 S-Nitrosylation
Y108 Phosphorylation
K110 Ubiquitination
K121 Ubiquitination
K124 Ubiquitination
K141 Ubiquitination
K150 Ubiquitination
K182 Acetylation
K182 Ubiquitination
K189 Acetylation
K197 Acetylation
K197 Ubiquitination
Y254 Phosphorylation
T256 Phosphorylation
K260 Ubiquitination
S279 Phosphorylation
K294 Ubiquitination
K297 Ubiquitination
K320 Ubiquitination
S322 Phosphorylation
K331 Ubiquitination
K381 Ubiquitination
T419 Phosphorylation
K421 Ubiquitination
K425 Ubiquitination
S508 Phosphorylation
K539 Ubiquitination
K545 Ubiquitination
S592 Phosphorylation
K597 Ubiquitination
S598 Phosphorylation
T603 Phosphorylation
K749 Ubiquitination
K756 Ubiquitination
K775 Ubiquitination
K802 Ubiquitination
K838 Ubiquitination
K903 Ubiquitination
Y911 Phosphorylation
K916 Ubiquitination
K939 Ubiquitination
K982 Ubiquitination
Y991 Phosphorylation
K1062 Acetylation
K1062 Ubiquitination
K1072 Ubiquitination
K1077 Ubiquitination
Y1078 Phosphorylation
T1092 Phosphorylation
K1107 Ubiquitination
K1216 Ubiquitination
K1221 Ubiquitination
K1234 Ubiquitination
K1248 Ubiquitination
K1251 Ubiquitination
C1265 S-Nitrosylation
C1278 S-Nitrosylation

Research Backgrounds

Function:

Involved in T-cell adhesion and p53/TP53-dependent induction of apoptosis. Does not bind RNA. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (By similarity).

Subcellular Location:

Cytoplasm. Nucleus. Cytoplasm>Perinuclear region. Cell junction>Synapse>Synaptosome.
Note: Highly expressed in the perinuclear regionand enriched in synaptosomes (By similarity). Treatment with leptomycin-B triggers translocation to the nucleus (PubMed:17245118).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in T-cells. Increased expression is observed in CD4(+) T-lymphocytes from patients with multiple sclerosis (at protein level).

Subunit Structure:

Component of the WAVE1 complex composed of ABI2, CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Interacts with FMR1, FXR1 and FXR2 (By similarity). Interacts with FMR1 isoform 6; the interaction occurs in a RNA-dependent manner. Interacts with RAC1 (activated form) which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1. Interacts with SHANK3; the interaction mediates the association of SHANK3 with the WAVE1 complex (By similarity). Interacts with TMEM108 (via N-terminus); the interaction associates TMEM108 with the WAVE1 complex (By similarity).

Family&Domains:

Belongs to the CYFIP family.

Research Fields

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Genetic Information Processing > Translation > RNA transport.

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