Phospho-IL-10R alpha (Tyr496) Antibody

WB
pElisa

1/2

Western blot analysis of Phospho-IL-10R alpha (Tyr496) Antibody expression in HuvEc cells lysates.

Product:	Phospho-IL-10R alpha (Tyr496) Antibody
Catalog:	AF3435
Description:	Rabbit polyclonal antibody to Phospho-IL-10R alpha (Tyr496)
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Pig, Horse, Rabbit, Dog, Chicken
Mol.Wt.:	63kDa; 63kD(Calculated).
Uniprot:	Q13651
RRID:	AB_2834877

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(100%), Horse(89%), Rabbit(89%), Dog(100%), Chicken(88%)

Clonality:

Polyclonal

Specificity:

Phospho-IL-10R alpha (Tyr496) Antibody detects endogenous levels of IL-10R alpha only when phosphorylated at Tyrosine 496.

RRID:

AB_2834877
Cite Format: Affinity Biosciences Cat# AF3435, RRID:AB_2834877.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD210; CDw210a; CDw210a antigen; HIL 10R; HIL10R; I10R1_HUMAN; IL 10R 1; IL 10R A; IL 10R; IL 10R1; IL-10 receptor subunit alpha; IL-10R subunit 1; IL-10R subunit alpha; IL-10R1; IL-10RA; IL10R; IL10R1; IL10RA; Interleukin 10 receptor alpha; Interleukin 10 receptor alpha chain; Interleukin-10 receptor subunit 1; Interleukin-10 receptor subunit alpha;

Immunogens

Immunogen:

Uniprot:

Q13651(I10R1_HUMAN) >>Visit HPA database.

Gene(ID):

IL10RA(3587)

Expression:

Q13651 I10R1_HUMAN:

Primarily expressed in hematopoetic cells including B-cells, T-cells, NK cells, monocytes and macrophages. Not expressed in non-hematopoetic cells such as fibroblasts or endothelial cells.

Description:

The protein encoded by this gene is a receptor for interleukin 10. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines.

Sequence:

Q13651 I10R1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLPCLVVLLAALLSLRLGSDAHGTELPSPPSVWFEAEFFHHILHWTPIPNQSESTCYEVALLRYGIESWNSISNCSQTLSYDLTAVTLDLYHSNGYRARVRAVDGSRHSNWTVTNTRFSVDEVTLTVGSVNLEIHNGFILGKIQLPRPKMAPANDTYESIFSHFREYEIAIRKVPGNFTFTHKKVKHENFSLLTSGEVGEFCVQVKPSVASRSNKGMWSKEECISLTRQYFTVTNVIIFFAFVLLLSGALAYCLALQLYVRRRKKLPSVLLFKKPSPFIFISQRPSPETQDTIHPLDEEAFLKVSPELKNLDLHGSTDSGFGSTKPSLQTEEPQFLLPDPHPQADRTLGNREPPVLGDSCSSGSSNSTDSGICLQEPSLSPSTGPTWEQQVGSNSRGQDDSGIDLVQNSEGRAGDTQGGSALGHHSPPEPEVPGEEDPAAVAFQGYLRQTRCAEEKATKTGCLEEESPLTDGLGPKFGRCLVDEAGLHPPALAKGYLKQDPLEMTLASSGAPTGQWNQPTEEWSLLALSSCSDLGISDWSFAHDLAPLGCVAAPGGLLGSFNSDLVTLPLISSLQSSE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Dog

100

Horse

Rabbit

Chicken

Bovine

Sheep

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13651 As Substrate

Q13651

Site	PTM Type	Enzyme	Source
K183	Acetylation		Uniprot
S268	Phosphorylation		Uniprot
S319	Phosphorylation		Uniprot
S323	Phosphorylation		Uniprot
S370	Phosphorylation		Uniprot
Y446	Phosphorylation	P23458 (JAK1) , P29597 (TYK2)	Uniprot
Y496	Phosphorylation	P29597 (TYK2) , P23458 (JAK1)	Uniprot

Research Backgrounds

Q13651_I10R1_HUMAN

Function:

Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well. In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively. These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy.

PTMs:

Phosphorylated. Phosphorylation of the cytoplasmic tail induced STAT3 activation.

Ubiquitinated by BTRC; ubiquitination leads to endocytosis and subsequent degradation of IL10RA.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Cytoplasm.

Tissue Specificity:

Primarily expressed in hematopoetic cells including B-cells, T-cells, NK cells, monocytes and macrophages. Not expressed in non-hematopoetic cells such as fibroblasts or endothelial cells.

Subunit Structure:

Interacts with IL10. Interacts with IL10RB. Interacts (via its cytoplasmic domain) with JAK1 (via N-terminus). Interacts with BTRC; this interaction leads to IL10RA ubiquitination and subsequent degradation. Interacts with STAT3 (By similarity).

(Microbial infection) Interacts with human cytomegalovirus protein IL10.

(Microbial infection) Interacts with Epstein-Barr virus protein IL10.

Family&Domains:

Belongs to the type II cytokine receptor family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

Restrictive clause

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For Research Use Only.
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Phospho-IL-10R alpha (Tyr496) Antibody - #AF3435