Collagen Type XXIII Antibody | Affinity Biosciences

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Western blot analysis of extracts from Mouse thymus tissue, using Collagen Type XXIII Antibody.

Western blot analysis of extracts from A2780 cells, using Collagen Type XXIII Antibody.

Product:	Collagen Type XXIII Antibody
Catalog:	DF12906
Description:	Rabbit polyclonal antibody to Collagen Type XXIII
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Dog
Mol.Wt.:	68 kDa, 80 kDa; 52kD(Calculated).
Uniprot:	Q86Y22
RRID:	AB_2845867

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Dog(80%)

Clonality:

Polyclonal

Specificity:

Collagen Type XXIII Antibody detects endogenous levels of total Collagen Type XXIII.

RRID:

AB_2845867
Cite Format: Affinity Biosciences Cat# DF12906, RRID:AB_2845867.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

COL23A1; Collagen alpha-1(XXIII) chain; Collagen type XXIII alpha 1; DKFZp434K0621; Procollagen type XXIII alpha 1;

Immunogens

Immunogen:

Uniprot:

Q86Y22(CONA1_HUMAN) >>Visit HPA database.

Gene(ID):

COL23A1(91522)

Sequence:

Q86Y22 CONA1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGPGERAGGGGDAGKGNAAGGGGGGRSATTAGSRAVSALCLLLSVGSAAACLLLGVQAAALQGRVAALEEERELLRRAGPPGALDAWAEPHLERLLREKLDGLAKIRTAREAPSECVCPPGPPGRRGKPGRRGDPGPPGQSGRDGYPGPLGLDGKPGLPGPKGEKGAPGDFGPRGDQGQDGAAGPPGPPGPPGARGPPGDTGKDGPRGAQGPAGPKGEPGQDGEMGPKGPPGPKGEPGVPGKKGDDGTPSQPGPPGPKGEPGSMGPRGENGVDGAPGPKGEPGHRGTDGAAGPRGAPGLKGEQGDTVVIDYDGRILDALKGPPGPQGPPGPPGIPGAKGELGLPGAPGIDGEKGPKGQKGDPGEPGPAGLKGEAGEMGLSGLPGADGLKGEKGESASDSLQESLAQLIVEPGPPGPPGPPGPMGLQGIQGPKGLDGAKGEKGASGERGPSGLPGPVGPPGLIGLPGTKGEKGRPGEPGLDGFPGPRGEKGDRSERGEKGERGVPGRKGVKGQKGEPGPPGLDQPCPVGPDGLPVPGCWHK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

Rabbit

Pig

Horse

Bovine

Sheep

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q86Y22 As Substrate

Q86Y22

Site	PTM Type	Source
R6	Methylation	Uniprot
K15	Ubiquitination	Uniprot
T248	Phosphorylation	Uniprot
S450	Phosphorylation	Uniprot
T467	Phosphorylation	Uniprot

Research Backgrounds

Q86Y22_CONA1_HUMAN

PTMs:

Undergoes proteolytic cleavage by furin protease to yield a 60 kDa soluble form that forms a homotrimer and exhibits a low affinity interaction with heparin.

Subcellular Location:

Cell membrane>Single-pass type II membrane protein>Extracellular side.

Subunit Structure:

Homotrimer.

References

1). Co-culture pellet of human Wharton’s jelly mesenchymal stem cells and rat costal chondrocytes as a candidate for articular cartilage regeneration: in vitro and in vivo study. Stem Cell Research & Therapy, 2022 (PubMed: 35907866) [IF=7.5]

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Collagen Type XXIII Antibody - #DF12906