CNOT6 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Jurkat cells, using CNOT6 Antibody.

Product:	CNOT6 Antibody
Catalog:	DF12902
Description:	Rabbit polyclonal antibody to CNOT6
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	63 kDa; 63kD(Calculated).
Uniprot:	Q9ULM6
RRID:	AB_2845863

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

CNOT6 Antibody detects endogenous levels of total CNOT6.

RRID:

AB_2845863
Cite Format: Affinity Biosciences Cat# DF12902, RRID:AB_2845863.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

A230103N10Rik; AA407540; AW456442; carbon catabolite repression 4 protein; Carbon catabolite repressor protein 4 homolog; CCR4; CCR4 carbon catabolite repression 4 like; CCR4 carbon catabolite repression 4-like; CCR4-NOT transcription complex subunit 6; CCR4-NOT transcription complex, subunit 6; Cnot6; CNOT6_HUMAN; Cytoplasmic deadenylase; EC 3.1.-.-; fa03c11; fc17f01; KIAA1194; MGC98472; OTTHUMP00000161544; wu:fa03c11; wu:fc17f01; zgc:65822;

Immunogens

Immunogen:

Uniprot:

Q9ULM6(CNOT6_HUMAN) >>Visit HPA database.

Gene(ID):

CNOT6(57472)

Sequence:

Q9ULM6 CNOT6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPKEKYEPPDPRRMYTIMSSEEAANGKKSHWAELEISGKVRSLSASLWSLTHLTALHLSDNSLSRIPSDIAKLHNLVYLDLSSNKIRSLPAELGNMVSLRELHLNNNLLRVLPFELGKLFQLQTLGLKGNPLTQDILNLYQEPDGTRRLLNYLLDNLSGTAKRITTEQPPPRSWIMLQEPDRTRPTALFSVMCYNVLCDKYATRQLYGYCPSWALNWDYRKKAIIQEILSCNADIVSLQEVETEQYYSFFLVELKERGYNGFFSPKSRARTMSEQERKHVDGCAIFFKTEKFTLVQKHTVEFNQLAMANSEGSEAMLNRVMTKDNIGVAVLLELRKESIEMPSGKPHLGTEKQLILVANAHMHWDPEYSDVKLVQTMMFLSEVKNIIDKASRNLKSSVLGEFGTIPLVLCADLNSLPDSGVVEYLSTGGVETNHKDFKELRYNESLTNFSCHGKNGTTNGRITHGFKLQSAYESGLMPYTNYTFDFKGIIDYIFYSKPQLNTLGILGPLDHHWLVENNISGCPHPLIPSDHFSLFAQLELLLPFLPQVNGIHLPGRR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Chicken

100

Rabbit

100

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9ULM6 As Substrate

Q9ULM6

Site	PTM Type	Source
S42	Phosphorylation	Uniprot
S49	Phosphorylation	Uniprot
T54	Phosphorylation	Uniprot
K118	Ubiquitination	Uniprot
K128	Ubiquitination	Uniprot
K162	Ubiquitination	Uniprot
S173	Phosphorylation	Uniprot
Y201	Phosphorylation	Uniprot
S212	Phosphorylation	Uniprot
K266	Ubiquitination	Uniprot
K278	Ubiquitination	Uniprot
T289	Phosphorylation	Uniprot
K291	Ubiquitination	Uniprot
S343	Phosphorylation	Uniprot
K345	Ubiquitination	Uniprot
K389	Ubiquitination	Uniprot
S445	Phosphorylation	Uniprot
K454	Ubiquitination	Uniprot

Research Backgrounds

Q9ULM6_CNOT6_HUMAN

Function:

Poly(A) nuclease with 3'-5' RNase activity. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Involved in mRNA decay mediated by the major-protein-coding determinant of instability (mCRD) of the FOS gene in the cytoplasm. In the presence of ZNF335, enhances ligand-dependent transcriptional activity of nuclear hormone receptors, including RARA. The increase of ligand-dependent ESR1-mediated transcription is much smaller, if any. Mediates cell proliferation and cell survival and prevents cellular senescence.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Predominantly cytoplasmic.

Subunit Structure:

Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits; the complex contains two deadenylase subunits, CNOT6 or CNOT6L, and CNOT7 or CNOT8. Interacts with CNOT7 and CNOT8. Interacts with UNR. Interacts with ZFP36L1 (via N-terminus). Interacts with ZNF335.

Family&Domains:

Belongs to the CCR4/nocturin family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.

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CNOT6 Antibody - #DF12902