KLK3 Antibody - #AF0246

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Product: KLK3 Antibody
Catalog: AF0246
Description: Rabbit polyclonal antibody to KLK3
Application: WB IHC
Reactivity: Human, Mouse
Mol.Wt.: 34kDa; 29kD(Calculated).
Uniprot: P07288
RRID: AB_2833421

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
KLK3 Antibody detects endogenous levels of total KLK3.
RRID:
AB_2833421
Cite Format: Affinity Biosciences Cat# AF0246, RRID:AB_2833421.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

antigen, prostate-specific; APS; Gamma seminoprotein; Gamma-seminoprotein; hK3; Kallikrein 3; Kallikrein related peptidase 3; Kallikrein-3; KLK 3; KLK2A1; Klk3; KLK3_HUMAN; P-30 antigen; P30 antigen; Prostate-specific antigen; Psa; Semenogelase; Seminin;

Immunogens

Immunogen:
Uniprot:

P07288(KLK3_HUMAN) >>Visit HPA database.

Gene(ID):
KLK3(354)
Description:
KLK3 Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. Belongs to the peptidase S1 family. Kallikrein subfamily.
Sequence:
MWVPVVFLTLSVTWIGAAPLILSRIVGGWECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCIRNKSVILLGRHSLFHPEDTGQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSHDLMLLRLSEPAELTDAVKVMDLPTQEPALGTTCYASGWGSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCSGDSGGPLVCNGVLQGITSWGSEPCALPERPSLYTKVVHYRKWIKDTIVANP

PTMs - P07288 As Substrate

Site PTM Type Enzyme
T143 Phosphorylation
T167 Phosphorylation
K251 Ubiquitination
K254 Ubiquitination

Research Backgrounds

Function:

Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Forms a heterodimer with SERPINA5.

Family&Domains:

Belongs to the peptidase S1 family. Kallikrein subfamily.

Research Fields

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

References

1). Pongamol Alleviates Neuroinflammation and Promotes Autophagy in Alzheimer's Disease by Regulating the Akt/mTOR Signaling Pathway. Journal of agricultural and food chemistry, 2024 (PubMed: 38841893) [IF=6.1]
2). Pyrroloquinoline quinone ameliorates liver injury in mice induced by cyclophosphamide. Environmental Science and Pollution Research, 2022 (PubMed: 34997497) [IF=5.8]
3). Dihydroartemisinin-ursodeoxycholic acid conjugate is a potential treatment agent for inflammatory bowel disease. International Immunopharmacology, 2023 (PubMed: 36842236) [IF=5.6]
4). Revealing the mechanism of ethyl acetate extracts of Semen Impatientis against prostate cancer based on network pharmacology and transcriptomics. Journal of ethnopharmacology, 2024 (PubMed: 38643863) [IF=5.4]
5). Sodium acetate alleviated high-carbohydrate induced intestinal inflammation by suppressing MAPK and NF-κB signaling pathways in Nile tilapia (Oreochromis niloticus). Fish & Shellfish Immunology, 2020 (PubMed: 31730927) [IF=4.7]
6). Sodium Danshensu Cream Promotes the Healing of Pressure Ulcers in Mice through the Nrf2/HO-1 and NF-κB Pathways. Pharmaceuticals, 2022 (PubMed: 36558999) [IF=4.6]

Application: WB    Species: Mouse    Sample:

Figure 7. Effect of SDSS on the protein levels of the NF-κB pathway (n = 5). (A) Western blot; the protein expression levels of NF-κB p65 (B), NF-κB p50 (C), IKKα (D), IKKβ (E), and IκBα (F) levels. * p < 0.05, ** p < 0.01 when compared to the control group. # p < 0.05, ## p < 0.01 when compared to the positive group.
7). Laminaria japonica fucoidan ameliorates cyclophosphamide‐induced liver and kidney injury possibly by regulating Nrf2/HO‐1 and TLR4/NF‐κB signaling pathways. Journal of the Science of Food and Agriculture, 2022 (PubMed: 34689333) [IF=4.1]
8). Capsaicin reduces blood glucose and prevents prostate growth by regulating androgen, RAGE/IGF-1/Akt, TGF-β/Smad signalling pathway and reversing epithelial-mesenchymal transition in streptozotocin-induced diabetic mice. Naunyn-Schmiedeberg's archives of pharmacology, 2024 (PubMed: 38700794) [IF=3.6]

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