CCT4 Antibody | Affinity Biosciences

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Western blot analysis of extracts from HeLa cells, using CCT4 Antibody.

Western blot analysis of extracts from various samples, using CCT4 Antibody.

Product:	CCT4 Antibody
Catalog:	DF12888
Description:	Rabbit polyclonal antibody to CCT4
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Rabbit, Dog, Xenopus
Mol.Wt.:	58 kDa, 50 kDa; 58kD(Calculated).
Uniprot:	P50991
RRID:	AB_2845849

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(91%), Zebrafish(89%), Bovine(91%), Rabbit(100%), Dog(91%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

CCT4 Antibody detects endogenous levels of total CCT4.

RRID:

AB_2845849
Cite Format: Affinity Biosciences Cat# DF12888, RRID:AB_2845849.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CCT delta; CCT4; CCTD; chaperonin containing t-complex polypeptide 1, delta subunit; chaperonin containing TCP1, subunit 4 (delta); SRB; Stimulator of TAR RNA binding; T complex protein 1 subunit delta; TCP-1-delta;

Immunogens

Immunogen:

Uniprot:

P50991(TCPD_HUMAN) >>Visit HPA database.

Gene(ID):

CCT4(10575)

Sequence:

P50991 TCPD_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPENVAPRSGATAGAAGGRGKGAYQDRDKPAQIRFSNISAAKAVADAIRTSLGPKGMDKMIQDGKGDVTITNDGATILKQMQVLHPAARMLVELSKAQDIEAGDGTTSVVIIAGSLLDSCTKLLQKGIHPTIISESFQKALEKGIEILTDMSRPVELSDRETLLNSATTSLNSKVVSQYSSLLSPMSVNAVMKVIDPATATSVDLRDIKIVKKLGGTIDDCELVEGLVLTQKVSNSGITRVEKAKIGLIQFCLSAPKTDMDNQIVVSDYAQMDRVLREERAYILNLVKQIKKTGCNVLLIQKSILRDALSDLALHFLNKMKIMVIKDIEREDIEFICKTIGTKPVAHIDQFTADMLGSAELAEEVNLNGSGKLLKITGCASPGKTVTIVVRGSNKLVIEEAERSIHDALCVIRCLVKKRALIAGGGAPEIELALRLTEYSRTLSGMESYCVRAFADAMEVIPSTLAENAGLNPISTVTELRNRHAQGEKTAGINVRKGGISNILEELVVQPLLVSVSALTLATETVRSILKIDDVVNTR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Xenopus

100

Pig

Bovine

Dog

Zebrafish

Horse

Sheep

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P50991 As Substrate

P50991

Site	PTM Type	Source
R8	Methylation	Uniprot
S9	Phosphorylation	Uniprot
R19	Methylation	Uniprot
K21	Methylation	Uniprot
Y24	Phosphorylation	Uniprot
R27	Methylation	Uniprot
K29	Ubiquitination	Uniprot
S36	Phosphorylation	Uniprot
K42	Ubiquitination	Uniprot
R49	Methylation	Uniprot
K55	Acetylation	Uniprot
K55	Ubiquitination	Uniprot
K59	Ubiquitination	Uniprot
K65	Acetylation	Uniprot
K65	Ubiquitination	Uniprot
K79	Ubiquitination	Uniprot
S95	Phosphorylation	Uniprot
K126	Ubiquitination	Uniprot
K139	Acetylation	Uniprot
K139	Methylation	Uniprot
K139	Ubiquitination	Uniprot
K143	Ubiquitination	Uniprot
S158	Phosphorylation	Uniprot
R160	Methylation	Uniprot
S170	Phosphorylation	Uniprot
S180	Phosphorylation	Uniprot
S184	Phosphorylation	Uniprot
T199	Phosphorylation	Uniprot
T201	Phosphorylation	Uniprot
S202	Phosphorylation	Uniprot
R206	Methylation	Uniprot
K209	Sumoylation	Uniprot
K213	Ubiquitination	Uniprot
K232	Ubiquitination	Uniprot
S234	Phosphorylation	Uniprot
S236	Phosphorylation	Uniprot
K243	Ubiquitination	Uniprot
K245	Ubiquitination	Uniprot
S254	Phosphorylation	Uniprot
S267	Phosphorylation	Uniprot
Y269	Phosphorylation	Uniprot
R274	Methylation	Uniprot
K288	Acetylation	Uniprot
K288	Ubiquitination	Uniprot
K292	Ubiquitination	Uniprot
C295	S-Nitrosylation	Uniprot
K302	Acetylation	Uniprot
K302	Ubiquitination	Uniprot
K319	Acetylation	Uniprot
K319	Ubiquitination	Uniprot
K321	Acetylation	Uniprot
K326	Acetylation	Uniprot
K343	Ubiquitination	Uniprot
K375	Ubiquitination	Uniprot
C379	S-Nitrosylation	Uniprot
S381	Phosphorylation	Uniprot
K384	Ubiquitination	Uniprot
T385	Phosphorylation	Uniprot
S393	Phosphorylation	Uniprot
K395	Acetylation	Uniprot
K395	Ubiquitination	Uniprot
C410	S-Nitrosylation	Uniprot
S444	Phosphorylation	Uniprot
Y449	Phosphorylation	Uniprot
S463	Phosphorylation	Uniprot
T464	Phosphorylation	Uniprot
K489	Acetylation	Uniprot
K489	Ubiquitination	Uniprot
K531	Ubiquitination	Uniprot
T538	Phosphorylation	Uniprot

Research Backgrounds

P50991_TCPD_HUMAN

Function:

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. The TRiC complex plays a role in the folding of actin and tubulin (Probable).

Subcellular Location:

Cytoplasm. Melanosome. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Cilium basal body.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Subunit Structure:

Component of the chaperonin-containing T-complex (TRiC), a heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter. Interacts with PACRG. Interacts with DNAAF4 (By similarity).

Family&Domains:

Belongs to the TCP-1 chaperonin family.

Restrictive clause

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For Research Use Only.
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CCT4 Antibody - #DF12888